公开(公告)号：US12216080B2

公开(公告)日：2025-02-04

申请号：US17560837

申请日：2021-12-23

Applicant: ProteinSimple

Inventor： Joshua I. Molho ,  Kelly Gardner ,  Danh C. Tran ,  Eric Jabart ,  David Nilson ,  Yuri Kouchnir ,  Michael Huston ,  Colin B. Kennedy ,  David John Roach

IPC: G01N27/453 ,  G01N27/447

Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

公开(公告)号：US12209291B2

公开(公告)日：2025-01-28

申请号：US18493087

申请日：2023-10-24

Applicant: ProteinSimple

Inventor： Jiaqi Wu ,  Christopher Heger

IPC: C12Q1/70 ,  C12N15/86 ,  C12Q1/00 ,  G16B20/00 ,  G16B40/00

Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.

公开(公告)号：US20240183820A1

公开(公告)日：2024-06-06

申请号：US18345798

申请日：2023-06-30

Applicant: ProteinSimple

Inventor： David J. ROACH ,  Tom Weisan YANG ,  Hui XU

IPC: G01N27/447 ,  G01N21/05 ,  G01N30/74

CPC classification number: G01N27/44791 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44747 ,  G01N21/05 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44795 ,  G01N30/74

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US20230126263A1

公开(公告)日：2023-04-27

申请号：US18087772

申请日：2022-12-22

Applicant: ProteinSimple

Inventor： Jiaqi WU ,  Christopher HEGER

IPC: C12Q1/70 ,  G16B20/00

Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.

公开(公告)号：US20220260525A1

公开(公告)日：2022-08-18

申请号：US17560837

申请日：2021-12-23

Applicant: ProteinSimple

Inventor： Joshua I. MOLHO ,  Kelly GARDNER ,  Danh C. TRAN ,  Eric JABART ,  David NILSON ,  Yuri KOUCHNIR ,  Michael HUSTON ,  Colin B. KENNEDY ,  David John ROACH

IPC: G01N27/447

Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

公开(公告)号：US11237131B2

公开(公告)日：2022-02-01

申请号：US15160636

申请日：2016-05-20

Applicant: ProteinSimple

Inventor： Joshua I. Molho ,  Kelly Gardner ,  Danh C. Tran ,  Eric Jabart ,  David Nilson ,  Yuri Kouchnir ,  Michael Huston ,  Colin B. Kennedy ,  David John Roach

IPC: G01N27/447

Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

公开(公告)号：US20210325377A1

公开(公告)日：2021-10-21

申请号：US16932441

申请日：2020-07-17

Applicant: ProteinSimple

Inventor： Irina Georgievna KAZAKOVA ,  Crystal TRAN ,  Jessica DERMODY ,  Annegret BOGE ,  Tom YANG

IPC: G01N33/536 ,  G01N33/533 ,  G01N33/535

Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.

公开(公告)号：US10753859B2

公开(公告)日：2020-08-25

申请号：US15793434

申请日：2017-10-25

Applicant: ProteinSimple

Inventor： Jiaqi Wu

IPC: G01N21/25 ,  G01N21/31 ,  A61K47/68 ,  G01N33/68

Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.

公开(公告)号：US20190285551A1

公开(公告)日：2019-09-19

申请号：US16423787

申请日：2019-05-28

Applicant: ProteinSimple

Inventor： Hui XU ,  Kenneth SWARTZ

IPC: G01N21/76 ,  G01N33/68 ,  G01N1/40 ,  G01N33/543

Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

公开(公告)号：US20180321189A1

公开(公告)日：2018-11-08

申请号：US16033808

申请日：2018-07-12

Applicant: ProteinSimple

Inventor： David J. ROACH ,  Tom Weisan YANG ,  Hui XU

IPC: G01N27/447

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.