公开(公告)号：US06682940B2

公开(公告)日：2004-01-27

申请号：US09909677

申请日：2001-07-20

申请人： Dan A. Pankowsky

发明人： Dan A. Pankowsky

IPC分类号： G01N33553

CPC分类号： G01N33/56966 ,  Y10S435/961 ,  Y10S436/824 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody-coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are contacted simultaneously or sequentially with a suspension of cells and bind the cells they are adapted to bind to form bead-cell complexes. Cells may bind to one or more microspheres. The bead-cell complexes are then separated from the suspension The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A method of quantitating a specific cell type is provided. A kit and apparatus for performing the method are also provided.

摘要翻译： 提供了细胞的单参数和多参数表征的方法，特别是细胞的免疫表型分析。 该方法优选使用适于结合特定类型细胞的抗体包被的微球。 一组或多组包被的微球体同时或依次与细胞悬浮液接触，并结合适于结合的细胞以形成珠细胞复合物。 细胞可以结合一个或多个微球。 然后将珠细胞复合物与悬浮液分离。复合物优选被染色，然后检查以表征细胞，优选与微球结合的细胞。 提供了定量细胞类型的方法。 还提供了用于执行该方法的套件和装置。

公开(公告)号：US06562239B2

公开(公告)日：2003-05-13

申请号：US10135148

申请日：2002-04-30

申请人： Jeffrey E. Foy ,  Eric B. Sweeney

发明人： Jeffrey E. Foy ,  Eric B. Sweeney

IPC分类号： G01N33553

CPC分类号： B03C1/035 ,  B03C1/288 ,  C12M47/02 ,  C12M47/04 ,  Y10S436/806

摘要： A magnetic biological particle separation device comprising positioning a magnetic arrangement comprising a first magnet to generate a first north-south magnetic field in a plane co-planer with a horizontal cross-sectional plane of a container, positioning a second magnet in the magnetic arrangement to generate a second north-south magnetic field substantially opposing the first north-south magnetic field in a plane co-planer with the horizontal cross-sectional plane of the container, positioning a third magnet in the magnetic arrangement to generate a third north-south magnetic field substantially perpendicular to the first and the second north-south magnetic fields, directed radially away from the container, in a plane co-planer with the horizontal cross-sectional plane of the container, and pouring from the container a fluid containing magnetic beads, without a substantial loss of the magnetic beads.

公开(公告)号：US06479302B1

公开(公告)日：2002-11-12

申请号：US09355555

申请日：1999-11-17

申请人： Bernd Dremel

发明人： Bernd Dremel

IPC分类号： G01N33553

CPC分类号： G01N33/54386 ,  G01N33/54333 ,  Y10S435/97 ,  Y10S435/971 ,  Y10S436/805 ,  Y10S436/806

摘要： The invention concerns a method for the immunological determination of an analyte in a sample using magnetic particles coated with the analyte to be determined or analyte-specific bonding partners and directly detectable non-magnetic particles coated with analyte-specific bonding partners or the analyte to be determined or using a non-magnetic substance which is indirectly detectable, and incubation of the reaction mixture. The method is characterized in that the magnetic particles are subsequently separated from the reaction mixture using a magnetic test strip and the analyte concentration is determined directly.

摘要翻译： 本发明涉及使用涂覆有待测分析物或分析物特异性结合伙伴的磁性颗粒和涂覆有分析物特异性结合伙伴或分析物的直接可检测非磁性颗粒来免疫测定样品中分析物的方法 确定或使用可间接检测的非磁性物质，以及培养反应混合物。 该方法的特征在于磁性颗粒随后使用磁性试纸从反应混合物中分离出来，分析物浓度直接测定。

公开(公告)号：US06468809B1

公开(公告)日：2002-10-22

申请号：US09497754

申请日：2000-02-04

申请人： Gary A. Prinz ,  Michael M. Miller

发明人： Gary A. Prinz ,  Michael M. Miller

IPC分类号： G01N33553

CPC分类号： G11C11/15 ,  G01N27/745 ,  G01N33/54326

摘要： A magnetic sensing element detects the presence of magnetic particles in a binding assay. The magnetic sensing element has at least one planar layer of electrically conductive ferromagnetic material that has an initial state in which the material has a circular magnetic moment within the plane of the layer. The magnetic sensing element has molecules of a first specific binding member attached to it. The device also includes a fluid test medium to which the magnetic sensing element is exposed during the course of a binding assay. The fluid test medium includes magnetizable particles that become immobilized during the assay in relation to the amount of analyte in the test medium. The relative size of the magnetic particle and the magnetic sensing element and the location of the molecules of the first specific binding member on the magnetic sensing element arc selected so that when the magnetic particle becomes immobilized with respect to the magnetic sensing element, the radial fringing field of the magnetic particle causes the magnetic moment of at least one layer of electrically conductive ferromagnetic material to shift from circular to radial, thereby causing a detectable change in the electrical resistance of the magnetic sensing element.

摘要翻译： 磁感应元件在结合测定中检测磁性颗粒的存在。 磁传感元件具有至少一个导电铁磁材料的平面层，其具有初始状态，其中该材料在该层的平面内具有圆形磁矩。 磁传感元件具有连接到其上的第一特定结合构件的分子。 该装置还包括流体测试介质，磁性感测元件在结合测定过程中暴露于其中。 流体测试介质包括在测定期间相对于测试介质中的分析物的量变得固定的可磁化颗粒。 选择磁性粒子和磁性感测元件的相对尺寸以及磁性感测元件上的第一特定结合构件的分子的位置，使得当磁性颗粒相对于磁敏元件固定时，径向条纹 磁性颗粒的磁场使得至少一层导电铁磁材料的磁矩从圆形向径向移动，从而导致磁感测元件的电阻的可检测变化。

公开(公告)号：US06461874B1

公开(公告)日：2002-10-08

申请号：US09491139

申请日：2000-01-26

申请人： Wei-Chao Ni ,  Daniel W. Eustace ,  Steve Chin-Shen Chang

发明人： Wei-Chao Ni ,  Daniel W. Eustace ,  Steve Chin-Shen Chang

IPC分类号： G01N33553

CPC分类号： G01N33/54333 ,  G01N33/5434 ,  G01N33/54393 ,  G01N2333/4731 ,  G01N2446/84 ,  Y10S435/963 ,  Y10S436/81 ,  Y10S530/815 ,  Y10T428/2991 ,  Y10T428/2998

摘要： It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used in immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and fewer discordant samples have been developed. Casein used at a concentration of 0.05-4.0 grams per gram of paramagnetic particle (optimally approximately 0.78-1.2 grams of casein per gram of magnetic particle) has been found to confer this benefit.

摘要翻译： 已经发现酪蛋白和酪蛋白的盐可用作BSA的替代物或作为BSA的替代物，作为用于免疫测定和用于分离所需分析物的其它结合测定法的固相的特别是磁性颗粒的材料。 通过使用酪蛋白，开发了具有改善的稳定性和较少不一致样品的免疫测定。 已经发现以每克顺磁性颗粒（最佳约0.78-1.2克酪蛋白/克磁性颗粒）浓度为0.05-4.0克的酪蛋白赋予这种益处。

公开(公告)号：US06447729B1

公开(公告)日：2002-09-10

申请号：US09479615

申请日：2000-01-07

申请人： Jukka Tuunanen

发明人： Jukka Tuunanen

IPC分类号： G01N33553

CPC分类号： B03C1/286 ,  B01L3/02 ,  B01L3/50853 ,  B01L2200/0657 ,  B01L2300/044 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/16 ,  B03C2201/18 ,  G01N33/54326 ,  G01N33/54366 ,  G01N35/0098 ,  G01N35/026 ,  G01N35/1065 ,  G01N35/109 ,  G01N2035/1055 ,  Y10S436/806 ,  Y10S436/808 ,  Y10T436/25375

摘要： An apparatus for separating magnetic particles from a solution includes a vessel with a smaller diameter lower portion and larger diameter upper portion, a magnetic remover and a positioner for the magnetic remover. The transition between the small and large diameter portions of the vessel facilitates separation of droplets from the magnetic remover as it is removed from the vessel, while still retaining magnetic particles.

摘要翻译： 用于从溶液中分离磁性颗粒的装置包括具有较小直径下部和较大直径上部的容器，磁性去除器和用于磁性去除器的定位器。 容器的小直径部分和大直径部分之间的过渡有助于从磁性去除器中分离出液滴，同时仍然保留磁性颗粒。

公开(公告)号：US06413420B1

公开(公告)日：2002-07-02

申请号：US09527797

申请日：2000-03-17

申请人： Jeffrey E. Foy ,  Eric B. Sweeney

发明人： Jeffrey E. Foy ,  Eric B. Sweeney

IPC分类号： G01N33553

CPC分类号： B03C1/035 ,  B03C1/288 ,  C12M47/02 ,  C12M47/04 ,  Y10S436/806

摘要： A magnetic biological particle separation device comprising a permanent magnet structure substantially surrounding a vessel containing a liquid and a biological sample in colloidal suspension in the liquid is described. The magnetic separation device comprises a window through which to view the liquid. The permanent magnet structure comprises a plurality of permanent magnets arranged in such a manner to provide for superpositioning of the external magnetic field generated by each magnet. The external magnetic field acts on magnetic beads attached to the biological sample, causing the sample to move toward the inner wall of the vessel. The permanent magnets do not completely surround the outside of the vessel to allow the liquid to be poured out of the vessel, substantially leaving the sample.

摘要翻译： 描述了一种磁性生物颗粒分离装置，其包括基本上围绕容器中的液体和胶体悬浮液中的生物样品的永久磁体结构。 磁分离装置包括用于观察液体的窗口。 永磁体结构包括多个永久磁铁，其以这样的方式布置以提供由每个磁体产生的外部磁场的叠加。 外部磁场作用于附着在生物样品上的磁珠，导致样品向容器的内壁移动。 永磁体不完全围绕容器的外部，以允许液体从容器中倒出，基本上留下样品。

公开(公告)号：US06342396B1

公开(公告)日：2002-01-29

申请号：US09355246

申请日：1999-09-17

申请人： Agnés Perrin ,  Alain Theretz ,  Bernard Mandrand

发明人： Agnés Perrin ,  Alain Theretz ,  Bernard Mandrand

IPC分类号： G01N33553

CPC分类号： G01N33/54326 ,  B01J2219/00648 ,  B01J2219/00659 ,  G01N33/54306 ,  Y10S436/801 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/824 ,  Y10T436/24

摘要： The present invention relates to a method for at least one of detecting, quantifying or isolating at least one analyte from a liquid medium in which the analyte is distributed and comprises providing a reagent comprised of particles having a receptor for an analyte fixed to the particles distributed in the medium and a capturing means having an exposed surface defining an active zone. An intermediate reagent is formed by the complex of the reagent with the analyte. A second receptor is fixed in the active zone to capture either the analyte bound by the reagent or the receptor (capture partners). The active zone serves as a site of isolation and concentration of the capture partners.

摘要翻译： 本发明涉及一种用于从分析物分布的液体介质中检测，定量或分离至少一种分析物的方法，并且包括提供由具有固定在分布的颗粒上的分析物的受体的颗粒组成的试剂 并且具有限定活动区域的暴露表面的捕获装置。 中间试剂由试剂与分析物的复合物形成。 第二受体固定在活性区中以捕获由试剂或受体（捕获配偶体）结合的分析物。 活动区域是捕获伙伴隔离和集中的场所。

公开(公告)号：US06291155B1

公开(公告)日：2001-09-18

申请号：US09262098

申请日：1999-03-04

申请人： Burkhard Raguse ,  Ronald John Pace ,  Lionel George King ,  Vijoleta Lucija Braach-Maksvytis ,  Bruce Cornell

发明人： Burkhard Raguse ,  Ronald John Pace ,  Lionel George King ,  Vijoleta Lucija Braach-Maksvytis ,  Bruce Cornell

IPC分类号： G01N33553

CPC分类号： C12Q1/002 ,  G01N33/5438 ,  Y10S435/817 ,  Y10S436/806

摘要： Electrode membrane combinations for use in biosensors to detect analytes in a sample and methods for making and storing same are disclosed. In one aspect, a method is provided for producing a first layer electrode membrane comprising: (1) Forming a solution containing Linker Lipid A, the disulfide of mercaptoacetic acid (MAAD) or similar molecule, linker Gramicidin B, membrane spanning lipid C (MSL-C) and membrane spanning lipid D (MSL-D) or other suitable linker molecules and other ion channel combinations; (2) Contacting an electrode containing a clean gold surface with the solution, the disulfide containing components in the solution thus adsorbing onto the gold surface of the electrode; (3) Rinsing the electrode with a suitable organic solvent; and (4) Removing the excess organic solvent used for rinsing.

摘要翻译： 公开了用于生物传感器检测样品中的分析物的电极膜组合以及用于制备和存储样品的方法。 一方面，提供了一种用于制备第一层电极膜的方法，其包括：（1）形成含有接头脂质A，巯基乙酸（MAAD）或类似分子的二硫化物的溶液，连接剂杀伤菌素B，跨越脂质C -C）和覆盖脂质D（MSL-D）或其它合适的接头分子和其它离子通道组合的膜;（2）将含有清洁金表面的电极与溶液接触，将溶液中的二硫化物的组分吸附在 电极的金表面;（3）用合适的有机溶剂冲洗电极; 和（4）除去用于漂洗的过量有机溶剂。

公开(公告)号：US06268223B1

公开(公告)日：2001-07-31

申请号：US09384356

申请日：1999-08-27

申请人： Ann H. Cornell-Bell ,  Kathleen S. Madden ,  Leslie A. Riblet

发明人： Ann H. Cornell-Bell ,  Kathleen S. Madden ,  Leslie A. Riblet

IPC分类号： G01N33553

CPC分类号： G01N33/6893 ,  G01N2800/324 ,  Y10S435/975

摘要： A method is disclosed for the rapid diagnosis of disorders characterized by an ischemic event, such as stroke, transient ischemic attack, head trauma, myocardial infarction or other insults resulting in interrupted cranial blood flow. The method involves detection of the presence of the gamma isoform of protein kinase C (PKCg) in peripheral blood, which signals damage to central nervous system tissue and at least transient breakdown of the blood brain barrier. The assay may be performed, e.g., by emergency medical personnel, in a time frame that allows treatment of the patient before permanent damage to the central nervous system occurs.

摘要翻译： 公开了用于快速诊断特征为缺血事件（例如中风，短暂性脑缺血发作，头部创伤，心肌梗塞或导致中断的颅血流量的其他侮辱）的疾病的方法。 该方法包括检测外周血中蛋白激酶C（PKCg）的γ同种型的存在，其表示对中枢神经系统组织的损伤，并且至少是血脑屏障的瞬时分解。 该测定可以例如由紧急医务人员在允许对中枢神经系统的永久性损伤之前发生患者治疗的时间范围内进行。