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105 条记录 (耗时 0.016 秒)

    METHOD FOR MEASURING BETA-GLUCAN, AND BETA-GLUCAN-BINDING PROTEIN FOR USE IN THE METHOD
    16.
    发明申请
    METHOD FOR MEASURING BETA-GLUCAN, AND BETA-GLUCAN-BINDING PROTEIN FOR USE IN THE METHOD 有权
    用于测量BETA-GLUCAN和BET-GLUCAN-BINDING蛋白的方法用于方法

    公开(公告)号：US20120009594A1

    公开(公告)日：2012-01-12

    申请号：US13257080

    申请日：2010-03-17

    申请人： Akito Yoneda

    发明人： Akito Yoneda

    IPC分类号： G01N33/53 ,  C12N9/26 ,  C07H21/04 ,  C12N1/21 ,  C12N1/19 ,  C12N15/63 ,  C12P21/06 ,  C12N5/10

    CPC分类号： G01N33/579 ,  C07K14/43509 ,  C12N9/6408 ,  G01N33/5308 ,  G01N33/56961 ,  G01N2333/38 ,  G01N2333/40 ,  G01N2333/43504 ,  G01N2400/24 ,  G01N2469/10 ,  Y10S435/935

    摘要： Disclosed are a method for measuring βG comprising the steps of bringing a sample into contact with a βG-binding protein 1 and a βG-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID NO:20, and having the β-glucan binding activity, to form a complex of the βG-binding protein 1, βG in the sample and the βG-binding protein 2, measuring quantity of the complex, and determining βG concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel βG-binding protein; a nucleic acid molecule encoding the βG-binding protein; and a method for producing the aforementioned βG-binding protein.

    摘要翻译： 本发明公开了一种测定G蛋白的方法，其包括使样品与G结合蛋白1和结合蛋白2接触的步骤，每个G结合蛋白2均包含与 SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8，SEQ ID NO：10，SEQ ID NO：14，SEQ ID NO：16，SEQ ID NO：18中任一项所示的氨基酸序列 或SEQ ID NO：20，并且具有葡聚糖结合活性，以形成样品中的G结合蛋白1和b结合蛋白与G结合蛋白2的复合物，测量量 的复合物，并根据复合物的数量确定样品中的G浓度; 用于所述方法的试剂和试剂盒; 一种新型的G结合蛋白; 编码G结合蛋白的核酸分子; 以及制备上述结合蛋白的方法。

    Vectors and method of penicillium chrysogenum transformation
    19.
    发明授权
    Vectors and method of penicillium chrysogenum transformation 失效
    产黄青霉转化载体及方法

    公开(公告)号：US4959327A

    公开(公告)日：1990-09-25

    申请号：US9713

    申请日：1987-02-02

    申请人： Florentina S. Sanchez ,  Victor R. Susan ,  Miguel A. P. Soto ,  Agustin P. A. Ortega

    发明人： Florentina S. Sanchez ,  Victor R. Susan ,  Miguel A. P. Soto ,  Agustin P. A. Ortega

    IPC分类号： C12N1/13 ,  C12N9/06 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/80

    CPC分类号： C12N9/0028 ,  C12N15/52 ,  C12N15/80 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  Y10S435/935

    摘要： Disclosed is a method for transforming Penicillium chrysogenum. More particularly, the method includes obtaining an auxotrophic mutant of P. chrysogenum and employing an exogeneous segment of P. chrysogenum DNA capable of complementing said auxotorph so as to restore prototrophy. The exogeneous DNA segment thus comprises a phenotypic marker indicating successful transformation of the mutant.The exogeneous complementary DNA segment may further be prepared in a recombinant plasmid vector. These plasmid vectors include, by way of example, the pPctrpCL and pPctrpC.sub.6 plasmids developed by Applicants. These transforming plasmid vectors and those having identifying characteristics thereof are suitable for use in the subject transformation process. The exogeneous complementary DNA segment comprises a gene encoding a selected biosynthetic enzyme. By way of example, these genes include trpC, pyr4, argB and NO.sup.- reductase, as well as other genes which encode metabolically required enzymes. The most preferred of these is trpC.

    摘要翻译： 公开了一种转化产黄青霉的方法。 更具体地，该方法包括获得产黄青霉的营养缺陷型突变体，并使用能够补充所述生长螺旋体的产黄青霉DNA的外源性片段，以恢复原养型。 因此，外源DNA片段包含表明突变体成功转化的表型标记。 外源互补DNA片段可以进一步在重组质粒载体中制备。 这些质粒载体包括例如由申请人开发的pPctrpCL和pPctrpC6质粒。 这些转化质粒载体和具有鉴定特征的质粒载体适用于受试者转化过程。 外源互补DNA片段包含编码所选生物合成酶的基因。 作为例子，这些基因包括trpC，pyr4，argB和NO-还原酶，以及编码代谢需要的酶的其它基因。 其中最优选的是trpC。