公开(公告)号：US4959327A

公开(公告)日：1990-09-25

申请号：US9713

申请日：1987-02-02

申请人： Florentina S. Sanchez ,  Victor R. Susan ,  Miguel A. P. Soto ,  Agustin P. A. Ortega

发明人： Florentina S. Sanchez ,  Victor R. Susan ,  Miguel A. P. Soto ,  Agustin P. A. Ortega

IPC分类号： C12N1/13 ,  C12N9/06 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/80

CPC分类号： C12N9/0028 ,  C12N15/52 ,  C12N15/80 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  Y10S435/935

摘要： Disclosed is a method for transforming Penicillium chrysogenum. More particularly, the method includes obtaining an auxotrophic mutant of P. chrysogenum and employing an exogeneous segment of P. chrysogenum DNA capable of complementing said auxotorph so as to restore prototrophy. The exogeneous DNA segment thus comprises a phenotypic marker indicating successful transformation of the mutant.The exogeneous complementary DNA segment may further be prepared in a recombinant plasmid vector. These plasmid vectors include, by way of example, the pPctrpCL and pPctrpC.sub.6 plasmids developed by Applicants. These transforming plasmid vectors and those having identifying characteristics thereof are suitable for use in the subject transformation process. The exogeneous complementary DNA segment comprises a gene encoding a selected biosynthetic enzyme. By way of example, these genes include trpC, pyr4, argB and NO.sup.- reductase, as well as other genes which encode metabolically required enzymes. The most preferred of these is trpC.

摘要翻译： 公开了一种转化产黄青霉的方法。 更具体地，该方法包括获得产黄青霉的营养缺陷型突变体，并使用能够补充所述生长螺旋体的产黄青霉DNA的外源性片段，以恢复原养型。 因此，外源DNA片段包含表明突变体成功转化的表型标记。 外源互补DNA片段可以进一步在重组质粒载体中制备。 这些质粒载体包括例如由申请人开发的pPctrpCL和pPctrpC6质粒。 这些转化质粒载体和具有鉴定特征的质粒载体适用于受试者转化过程。 外源互补DNA片段包含编码所选生物合成酶的基因。 作为例子，这些基因包括trpC，pyr4，argB和NO-还原酶，以及编码代谢需要的酶的其它基因。 其中最优选的是trpC。