摘要:
This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.
摘要:
This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.
摘要:
The present invention relates to polypeptides having mutanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to oral cavity compositions and methods for degrading mutan.
摘要:
A process for producing a variety of chemical products, e.g., ethanol, by fermentation in which the product is removed from the fermentation medium as it is formed by liquid-liquid extraction using an extractant for the product which is immiscible with water. The extractant employed is chosen from the following groups: (A) double bond unsaturated aliphatic alcohols having 12 or more carbon atoms; (B) saturated branched chain aliphatic alcohols having 14 or more carbon atoms or mixtures thereof; (C) double bond unsaturated aliphatic acids having 12 or more carbon atoms; (D) aliphatic and aromatic mono-, di- or tri-esters having 12 or more carbon atoms, other than dibutyl phthalate; (E) aliphatic noncyclic ketones and aliphatic aldehydes having 12 or more carbon atoms; and (F) mixtures of extractants from groups (A) to (E) above or mixtures of at least one of the above extractants and at least one other extractant. These extractants are non-toxic to microorganisms used for fermentation, have little tendency to form emulsions and are otherwise suited for the process. The liquid-liquid extraction may thus be carried out in situ in the fermentor, preferably in a continuous stirred tank fermentor.
摘要:
THE PRESENT DISCLOSURE IS DIRECTED TO A METHOD WITH A TWO-FOLD OBJECTIVE. AS IS WELL KNOWN, PULP MILL WASTES WHICH ARE PRIMARILY COMPOSED OF LIGNIN ARE DARK IN COLOR AND EXTREMELY RESISTANT TO BIODEGRADATION. ACCORDINTLY, THE DISCHARGE OF THESE WASTES TO ANY NATURAL WATERWAYS IS LIMITED BY LEGISLATION. THE PRESENT INVENTION PROVIDES A METHOD FOR IMPROVING THE COLOR CHARACTERISTICS OF THE WASTE AND FOR UTILIZING THE PRODUCT OR MATERIAL OBTAINED THEREBY TO PRODUCE VALUABLE AND UTILIZABLE MATERIALS OR CHEMICALS. BASICALLY, THE METHOD ENTAILS CONTACTING AN OXIDATIVELY TREATED WASTE AND, MORE SPECIFICIALLY, AN OXIDATIVELY TREATED WASTE LIGNIN WITH A MICROBIAL POPULATION FOR A TIME AND AT CONDITIONS NECESSARY TO CONVERT SAID OXIDATIVELY TREATED LIGNIN TO THE UTILIZABLE END PRODUCTS. EXAMPLES OF UTILIZABLE PRODUCTS WHICH CAN BE OBTAINED IN THIS MANNER ARE ORGANIC ACIDS, BIOPOLERS, PROTEINS, ANTINBIOTICS, STEROIDS, VITAMINS, FERTILIZERS, ETC.