知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

61 条记录 (耗时 0.033 秒)

    Methods for producing isobutene from 3-methylcrotonic acid
    11.
    发明授权
    Methods for producing isobutene from 3-methylcrotonic acid 有权

    公开(公告)号：US12084704B2

    公开(公告)日：2024-09-10

    申请号：US16612065

    申请日：2018-04-19

    申请人： Global Bioenergies

    发明人： Romain Chayot ,  Mathieu Allard ,  Maria Anissimova

    IPC分类号： C12P5/02

    CPC分类号： C12P5/026 ,  C12Y205/01 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y207/06002 ,  C12Y207/06003 ,  C12Y401/01 ,  C12Y503/03002

    摘要： Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl phosphate (DMAP) into a flavin-derived cofactor, wherein said method further comprises providing said DMAP enzymatically by: (i) the enzymatic conversion of dimethylallyl pyrophosphate (DMAPP) into said DMAP; or (ii) a single enzymatic step in which prenol is directly enzymatically converted into said DMAP; or (iii) two enzymatic steps comprising: first enzymatically converting DMAPP into prenol; and then enzymatically converting the thus obtained prenol into said DMAP; or (iv) the enzymatic conversion of isopentenyl monophosphate (IMP) into said DMAP, or by a combination of any one of (i) to (iv). Moreover, described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl pyrophosphate (DMAPP), wherein said method further comprises providing said DMAPP enzymatically by: (v) the enzymatic conversion of isopentenyl pyrophosphate (IPP) into said DMAPP; or (vi) the enzymatic conversion of dimethylallyl phosphate (DMAP) into said DMAPP; or (vii) the enzymatic conversion of prenol into said DMAPP; (viii) or by a combination of any one of (v) to (vii). Moreover, described are methods for providing said flavin cofactor enzymatically by the enzymatic conversion of riboflavin into flavin mononucleotide (FMN).

    METHODS FOR STABILIZING PRODUCTION OF ACETYL-COENZYME A DERIVED COMPOUNDS
    20.
    发明申请
    METHODS FOR STABILIZING PRODUCTION OF ACETYL-COENZYME A DERIVED COMPOUNDS 审中-公开
    用于稳定乙酰辅酶A衍生化合物生产的方法

    公开(公告)号：US20150299713A1

    公开(公告)日：2015-10-22

    申请号：US14419609

    申请日：2013-08-07

    申请人： Amyris, Inc. ,  Total Marketing Services

    发明人： Hanxiao Jiang ,  Adam Meadows

    IPC分类号： C12N15/52 ,  C12N9/88 ,  C12N9/10 ,  C12P5/00 ,  C12N9/90

    CPC分类号： C12N15/52 ,  C12N1/20 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12P5/007 ,  C12Y205/01 ,  C12Y402/03047 ,  C12Y503/03002

    摘要： The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

    摘要翻译： 本公开涉及在微生物宿主细胞中用于产生异源非分解代谢化合物的开关的用途。 一方面，本文提供了在需氧条件下随后微量营养条件下连续培养时更稳定地产生非分解代谢化合物的遗传修饰微生物，以及通过在这种培养条件下培养经遗传修饰的微生物生产非分解代谢化合物的方法。 在另一方面，本文提供的是在麦芽糖存在下连续培养，随后还原或不存在麦芽糖时更稳定地产生非分解代谢化合物的遗传修饰的微生物，以及通过培养基因修饰的微生物生产非分解代谢化合物的方法 这样的文化条件。