公开(公告)号：US09765327B2

公开(公告)日：2017-09-19

申请号：US15183191

申请日：2016-06-15

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor Slukvin ,  Irina Elcheva

IPC: C12N5/00 ,  C12N15/11 ,  C12N5/0789 ,  C12N15/00 ,  C12N15/66

CPC classification number: C12N5/0606 ,  C12N5/0608 ,  C12N5/0647 ,  C12N15/00 ,  C12N15/11 ,  C12N15/66 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2740/16043

Abstract: Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

公开(公告)号：US20240191189A1

公开(公告)日：2024-06-13

申请号：US18537479

申请日：2023-12-12

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor Slukvin ,  Akhilesh Kumar ,  Saritha Sandra D'Souza

IPC: C12N5/0783 ,  C12N5/0781

CPC classification number: C12N5/0636 ,  C12N5/0635 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2513/00

Abstract: The present disclosure provides reagents and methods for producing arterial hemogenic endothelium, hematopoietic progenitor, and lymphoid cell cultures. Pharmaceutical compositions comprising arterial hemogenic endothelium cells, hematopoietic progenitor cells, and lymphoid cells produced by the reagents and methods of the invention and therapeutic methods using these pharmaceutical compositions are also provided.

公开(公告)号：US20240156963A1

公开(公告)日：2024-05-16

申请号：US18302636

申请日：2023-04-18

Applicant: Wisconsin Alumni Research Foundation

Inventor： James Thomson ,  Jue Zhang ,  Igor Slukvin ,  Aditi Majumder ,  Ho Sun Jung

IPC: A61K39/00 ,  A61P35/00 ,  C07K14/725 ,  C07K16/30 ,  C12N5/0783 ,  C12N5/0786 ,  C12N5/0787

CPC classification number: A61K39/464471 ,  A61K39/461 ,  A61K39/4613 ,  A61K39/4614 ,  A61K39/4631 ,  A61P35/00 ,  C07K14/7051 ,  C07K16/3084 ,  C12N5/0642 ,  C12N5/0645 ,  C12N5/0646 ,  C12N2506/45 ,  C12N2510/00

Abstract: This disclosure provides genetically engineered immune cells that express an anti-GD2 chimeric antigen receptor, methods of generating these cells, and methods of treating tumors using the genetically engineered cells.

公开(公告)号：US20230338420A1

公开(公告)日：2023-10-26

申请号：US18165244

申请日：2023-02-06

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor Slukvin ,  Aditi Majumder ,  Portia Smith ,  Anna Huttenlocher ,  David Bennin

IPC: A61K35/15 ,  C12N5/0786 ,  C12N5/0787 ,  C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  A61K39/395 ,  A61P35/00

CPC classification number: A61K35/15 ,  C12N5/0645 ,  C12N5/0642 ,  C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  A61K39/39558 ,  A61P35/00 ,  C12N2506/45 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/22 ,  C12N2501/145 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2310/20 ,  C12N2800/80

Abstract: The disclosure generally relates to methods for producing macrophages and neutrophils serum-free and feeder-free conditions from SIRPα inhibited pluripotent stem cells. The disclosure further relates to SIRPα inhibited macrophages and neutrophils and uses thereof.

公开(公告)号：US20230323301A1

公开(公告)日：2023-10-12

申请号：US18296895

申请日：2023-04-06

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Anna Huttenlocher ,  Igor Slukvin ,  Morgan Giese ,  Ho Sun Jung ,  David Bennin

IPC: C12N5/0787 ,  C12N5/074 ,  C12N9/22 ,  C12N9/12 ,  C07K14/705 ,  C07K14/74

CPC classification number: C12N5/0642 ,  C12N5/0696 ,  C12N9/22 ,  C12N9/12 ,  C07K14/70532 ,  C07K14/70539 ,  C12N5/0031

Abstract: This disclosure provides methods for producing neutrophils under serum-free and feeder-free conditions from protein tyrosine phosphatase 1B (PTP1b)-inhibited pluripotent stem cells. The disclosure further provides PTP1b inhibited neutrophils and uses thereof. Also disclosed are methods for producing human neutrophil-DC hybrid cells and human neutrophil-DC hybrid cells produced thereby.

公开(公告)号：US11499137B2

公开(公告)日：2022-11-15

申请号：US16143112

申请日：2018-09-26

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor Slukvin ,  Irina Elcheva

IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/0789 ,  C12N15/00 ,  C12N15/11 ,  C12N15/66 ,  C12N5/071

Abstract: Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

公开(公告)号：US20220228118A1

公开(公告)日：2022-07-21

申请号：US17715889

申请日：2022-04-07

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Igor Slukvin ,  Gene lchiro Uenishi

IPC: C12N5/0789 ,  C12N5/0783 ,  C12N5/071 ,  C12N5/095 ,  C12N5/00

Abstract: Methods, kits and compositions for differentiating pluripotent stem cells into cells of endothelial and hematopoietic lineages are disclosed.

公开(公告)号：US20210380947A1

公开(公告)日：2021-12-09

申请号：US17403202

申请日：2021-08-16

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Igor Slukvin ,  Akhilesh Kumar

IPC: C12N5/077 ,  A61K35/34 ,  A61K35/44

Abstract: The present invention relates generally to methods and compositions useful for therapeutic vascular tissue engineering. In particular, the present invention provides methods for generating substantially pure populations of vasculogenic cells from human mesenchymal progenitors, and methods and compositions for clinical applications in the field of regenerative medicine.

公开(公告)号：US20180258398A1

公开(公告)日：2018-09-13

申请号：US15917504

申请日：2018-03-09

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor Slukvin ,  Gene Ichiro Uenishi

IPC: C12N5/0789 ,  C12N5/0783 ,  C12N5/00 ,  C12N5/071 ,  C12N5/095

Abstract: Methods and compositions for differentiating pluripotent stem cells into cells of endothelial and hematopoietic lineages are disclosed.

公开(公告)号：US20160355786A1

公开(公告)日：2016-12-08

申请号：US15183191

申请日：2016-06-15

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor Slukvin ,  Irina Elcheva

IPC: C12N5/0789

CPC classification number: C12N5/0606 ,  C12N5/0608 ,  C12N5/0647 ,  C12N15/00 ,  C12N15/11 ,  C12N15/66 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2740/16043

Abstract: Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

Abstract translation: 本文描述的是通过在本文所述的转录因子组合的hPSC中强制表达，诱导将人多能干细胞（hPSC）分化为具有泛骨髓潜能或限制性潜力的血细胞内皮的方法和相关组合物。