-
公开(公告)号：US20160199179A1
公开(公告)日：2016-07-14
申请号：US14987601
申请日：2016-01-04
Applicant: Wake Forest University Health Sciences
Inventor： Anthony Atala , James Yoo
CPC classification number: A61F2/2415 , A61L27/3604 , A61L27/3641 , A61L27/3808 , A61L27/3826 , A61L27/383 , A61L27/3839 , A61L27/3886 , A61L27/507 , B82Y5/00 , B82Y10/00 , C12N5/0068 , C12N5/069 , C12N2533/90
Abstract: The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.
Abstract translation: 本发明涉及通过预处理由分离的祖细胞分化的内皮细胞和平滑肌细胞的基质来制备人造心脏瓣膜的方法。 这些细胞接种的基质暴露于模拟通过心脏的血液流动以产生类似于天然心脏瓣膜的组织工程心脏瓣膜的流体条件。
-
公开(公告)号：US11786581B2
公开(公告)日：2023-10-17
申请号：US17105156
申请日：2020-11-25
Applicant: Wake Forest University Health Sciences
Inventor： James Yoo , Sang Jin Lee , Anthony Atala , Mark Van Dyke
CPC classification number: A61K38/39 , A61K9/0024 , A61K38/4886 , A61L27/24 , A61L27/52 , A61L27/54 , A61L2300/41 , A61L2300/434 , A61L2400/06 , A61L2430/26 , A61K38/39 , A61K2300/00 , A61K38/4886 , A61K2300/00
Abstract: The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.
-
Patent Agency Ranking
13.发明申请BIOREACTOR SYSTEM AND METHOD OF ENHANCING FUNCTIONALITY OF MUSCLE CULTURED IN VITRO 审中-公开生物反馈系统和增强体内培养的肌肉功能的方法公开(公告)号：US20170035551A1
公开(公告)日：2017-02-09
申请号：US15298382
申请日：2016-10-20
Applicant: Wake Forest University Health Sciences
Inventor： James Yoo , Joel Stitzel , Anthony Atala , George Christ
CPC classification number: A61F2/08 , A61F2002/0894 , A61L27/3604 , A61L27/367 , A61L27/3683 , A61L27/3691 , A61L27/3826 , A61L27/3895 , A61L2430/30 , A61L2430/34 , C12M21/08 , C12M35/04 , C12N5/0658 , C12N2527/00 , C12N2539/00
Abstract: A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.
Abstract translation: 从前体肌细胞体外产生有组织的骨骼肌组织的方法包括：（a）在组织培养基中的支持体上提供前体肌细胞; 然后（b）在第一时间段期间沿着第一轴线循环地拉伸和松弛支撑件至少两次; 然后（c）任选地，但优选在第二时间段内将载体保持在基本上静止的位置; 然后（d）重复步骤（b）和（c）足以增强在载体上形成的组织的功能性和/或从前体肌细胞在固体支持物上产生有组织的骨骼肌组织的次数。
-
公开(公告)号：US20150025031A1
公开(公告)日：2015-01-22
申请号：US14382921
申请日：2013-03-05
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： James Yoo , Sang Jin Lee , Jachyun Kim , Anthony Atala
IPC: A61K31/7076
CPC classification number: A61K31/7076 , C12N5/0018 , C12N2500/40 , C12N2501/73
Abstract: The present invention provides a system and method of maintaining and/or increasing cell viability by downregulating cellular metabolic rate under hypoxic conditions, wherein the availability of adenosine or derivatives thereof in the cell is increased and/or prolonged. The present invention also relates to a system and method of prolonging the survival of implanted cells that are under hypoxic condition until host neovascularization is achieved, wherein the availability of adenosine or derivatives thereof in the cell is increased and/or prolonged. The present invention also provides a system and method of maintaining and/or increasing cell viability by downregulating cellular metabolic rate under hypoxic conditions, wherein at least one purine metabolism enzyme inhibitor is applied to the cell.
Abstract translation: 本发明提供通过在低氧条件下下调细胞代谢速率来维持和/或增加细胞活力的系统和方法，其中细胞中腺苷或其衍生物的可用性增加和/或延长。 本发明还涉及延长在缺氧条件下进行宿主新生血管形成的植入细胞的存活的系统和方法，其中细胞中腺苷或其衍生物的可用性增加和/或延长。 本发明还提供了通过在缺氧条件下下调细胞代谢速率来维持和/或增加细胞活力的系统和方法，其中至少一种嘌呤代谢酶抑制剂被施用于细胞。
-
公开(公告)号：US11305038B2
公开(公告)日：2022-04-19
申请号：US15747352
申请日：2016-07-21
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： James Yoo , In Kap Ko , Jennifer Huling , Anthony Atala
IPC: A61L27/56 , A61K35/44 , A61L27/24 , A61L27/38 , A61L27/50 , A61L27/52 , A61K35/33 , A61K35/34 , A61K35/39 , A61K35/407 , A61L27/20
Abstract: The present application relates to biomimetic three-dimensional (3D) scaffolds, constructs and methods of making the same. The three-dimensional scaffold can include a sacrificial internal cast and a durable external scaffold material, wherein the durable external scaffold material comprises a biocompatible material which completely surrounds the sacrificial internal cast and wherein the sacrificial internal cast be removed to yield a branching 3D network of hollow, vessel-like tubes that substantially mimics a native tissue or organ.
-
公开(公告)号：US20180214604A1
公开(公告)日：2018-08-02
申请号：US15938706
申请日：2018-03-28
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： Anthony Atala , James Yoo , In Kap Ko
IPC: A61L27/36 , A61L27/38 , A61K35/34 , A61K38/18 , A61K45/06 , A61K47/34 , A61K47/42 , A61L27/18 , C12N5/077 , C12N5/00 , A61L27/54 , A61L27/24 , A61K9/00
CPC classification number: A61L27/3604 , A61K9/0034 , A61K35/34 , A61K38/18 , A61K45/06 , A61K47/34 , A61K47/42 , A61L27/18 , A61L27/24 , A61L27/3683 , A61L27/3687 , A61L27/3834 , A61L27/54 , A61L2300/414 , A61L2400/06 , A61L2430/30 , C12N5/0062 , C12N5/0659 , C12N2513/00 , C12N2533/30 , C12N2533/54 , A61K2300/00 , C08L67/04
Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.
-
公开(公告)号：US20140242125A1
公开(公告)日：2014-08-28
申请号：US14239416
申请日：2012-08-15
Applicant: Wake Forest University Health Sciences
Inventor： Anthony Atala , James Yoo , In Kap Ko
CPC classification number: A61L27/3604 , A61K9/0034 , A61K35/34 , A61K38/18 , A61K45/06 , A61K47/34 , A61K47/42 , A61L27/18 , A61L27/24 , A61L27/3683 , A61L27/3687 , A61L27/3834 , A61L27/54 , A61L2300/414 , A61L2400/06 , A61L2430/30 , C12N5/0062 , C12N5/0659 , C12N2513/00 , C12N2533/30 , C12N2533/54 , A61K2300/00 , C08L67/04
Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.
Abstract translation: 本发明涉及用于获得用于移植的均匀尺寸的肌纤维片段的方法和组合物。 这些肌纤维碎片能够重建成沿着原生肌肉定向的长纤维。 植入的肌肉细胞与天然血管和神经网络结合，如组织学和免疫组织化学所证实的。 本发明是特别有利的，因为可以从供体部位收获自体肌肉，将其加工并注射到手术室中的靶位点。 碎片肌肉纤维可以容易地集成在宿主内。
-
-
-
-
-
-
Search Results
17
records
(took 0.02 seconds)
