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公开(公告)号：US20190153382A1
公开(公告)日：2019-05-23
申请号：US16227690
申请日：2018-12-20
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： James Yoo , Tao Xu , Anthony Atala
Abstract: A method of forming an array of viable cells is carried out by ink-jet printing a cellular composition containing said cells on a substrate. At least two different types of viable mammalian cells are printed on the substrate, the at least two different types of viable mammalian cells selected to together form a tissue. In some embodiments at least three or four different viable mammalian cells are printed on the substrate, the cells selected to together form a tissue. In some embodiments one of the viable mammalian cell types is a stem cell. In some embodiments the method further comprises printing at least one support compound on the substrate, the support compound selected to form a tissue together with said cells. In some embodiments the method further comprises printing at least one growth factor on the substrate, the growth factor selected to cause the cells to form a tissue.
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公开(公告)号：US09801713B2
公开(公告)日：2017-10-31
申请号：US14987601
申请日：2016-01-04
Applicant: Wake Forest University Health Sciences
Inventor： Anthony Atala , James Yoo
CPC classification number: A61F2/2415 , A61L27/3604 , A61L27/3641 , A61L27/3808 , A61L27/3826 , A61L27/383 , A61L27/3839 , A61L27/3886 , A61L27/507 , B82Y5/00 , B82Y10/00 , C12N5/0068 , C12N5/069 , C12N2533/90
Abstract: The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.
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公开(公告)号：US09757225B2
公开(公告)日：2017-09-12
申请号：US15298382
申请日：2016-10-20
Applicant: Wake Forest University Health Sciences
Inventor： James Yoo , Joel D. Stitzel, Jr. , Anthony Atala , George Christ
CPC classification number: A61F2/08 , A61F2002/0894 , A61L27/3604 , A61L27/367 , A61L27/3683 , A61L27/3691 , A61L27/3826 , A61L27/3895 , A61L2430/30 , A61L2430/34 , C12M21/08 , C12M35/04 , C12N5/0658 , C12N2527/00 , C12N2539/00
Abstract: A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.
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公开(公告)号：US09879278B2
公开(公告)日：2018-01-30
申请号：US14382816
申请日：2013-02-27
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： Baisong Lu , Qingguo Zhao , James Yoo , Anthony Atala
IPC: C12N15/85
CPC classification number: C12N15/85 , C12N2800/40 , C12N2840/007
Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.
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公开(公告)号：US20150079682A1
公开(公告)日：2015-03-19
申请号：US14382816
申请日：2013-02-27
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： Baisong Lu , Qingguo Zhao , James Yoo , Anthony Atala
IPC: C12N15/85
CPC classification number: C12N15/85 , C12N2800/40 , C12N2840/007
Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.
Abstract translation: 本发明包括用于在细胞子集中选择性表达靶基因的组合物和方法。 在某些实施方案中，本发明包括构建体，其包含第一核酸序列，其包含附加型维持元件和包含靶基因的第二核酸序列，其中所述附加型维持元件的表达由组成型启动子调节， 靶基因由非组成型启动子调控。 无论目标基因是否在细胞中表达，该构建体能够保持附加型状态。
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公开(公告)号：US11013828B2
公开(公告)日：2021-05-25
申请号：US15938706
申请日：2018-03-28
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： Anthony Atala , James Yoo , In Kap Ko
IPC: A61L27/36 , A61K38/18 , A61L27/18 , A61L27/24 , A61L27/38 , A61K35/34 , A61K45/06 , A61K47/34 , A61K47/42 , A61L27/54 , C12N5/00 , C12N5/077 , A61K9/00
Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.
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公开(公告)号：US20180140678A1
公开(公告)日：2018-05-24
申请号：US15640063
申请日：2017-06-30
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： James Yoo , Sang Jin Lee , Anthony Atala , Mark Van Dyke
CPC classification number: A61K38/39 , A61K9/0024 , A61K38/4886 , A61L27/24 , A61L27/52 , A61L27/54 , A61L2300/41 , A61L2300/434 , A61L2400/06 , A61L2430/26 , A61K2300/00
Abstract: The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.
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公开(公告)号：US09962466B2
公开(公告)日：2018-05-08
申请号：US15159219
申请日：2016-05-19
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： Anthony Atala , James Yoo , In Kap Ko
IPC: C12N5/077 , A61L27/36 , A61K35/34 , A61K45/06 , A61K47/34 , A61K47/42 , A61K38/18 , A61L27/18 , A61L27/24 , A61L27/38 , A61L27/54 , C12N5/00 , A61K9/00
CPC classification number: A61L27/3604 , A61K9/0034 , A61K35/34 , A61K38/18 , A61K45/06 , A61K47/34 , A61K47/42 , A61L27/18 , A61L27/24 , A61L27/3683 , A61L27/3687 , A61L27/3834 , A61L27/54 , A61L2300/414 , A61L2400/06 , A61L2430/30 , C12N5/0062 , C12N5/0659 , C12N2513/00 , C12N2533/30 , C12N2533/54 , A61K2300/00 , C08L67/04
Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.
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公开(公告)号：US20170333177A1
公开(公告)日：2017-11-23
申请号：US15667986
申请日：2017-08-03
Applicant: Wake Forest University Health Sciences
Inventor： James Yoo , Joel D. Stitzel, JR. , Anthony Atala , George Christ
CPC classification number: A61F2/08 , A61F2002/0894 , A61L27/3604 , A61L27/367 , A61L27/3683 , A61L27/3691 , A61L27/3826 , A61L27/3895 , A61L2430/30 , A61L2430/34 , C12M21/08 , C12M35/04 , C12N5/0658 , C12N2527/00 , C12N2539/00
Abstract: A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.
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公开(公告)号：US20160296667A1
公开(公告)日：2016-10-13
申请号：US15159219
申请日：2016-05-19
Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES
Inventor： Anthony Atala , James Yoo , In Kap Ko
CPC classification number: A61L27/3604 , A61K9/0034 , A61K35/34 , A61K38/18 , A61K45/06 , A61K47/34 , A61K47/42 , A61L27/18 , A61L27/24 , A61L27/3683 , A61L27/3687 , A61L27/3834 , A61L27/54 , A61L2300/414 , A61L2400/06 , A61L2430/30 , C12N5/0062 , C12N5/0659 , C12N2513/00 , C12N2533/30 , C12N2533/54 , A61K2300/00 , C08L67/04
Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.
Abstract translation: 本发明涉及用于获得用于移植的均匀尺寸的肌纤维片段的方法和组合物。 这些肌纤维碎片能够重建成沿着原生肌肉定向的长纤维。 植入的肌肉细胞与天然血管和神经网络结合，如组织学和免疫组织化学所证实的。 本发明是特别有利的，因为可以从供体部位收获自体肌肉，将其加工并注射到手术室中的靶位点。 碎片肌肉纤维可以容易地集成在宿主内。
