公开(公告)号：US11305038B2

公开(公告)日：2022-04-19

申请号：US15747352

申请日：2016-07-21

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： James Yoo ,  In Kap Ko ,  Jennifer Huling ,  Anthony Atala

IPC: A61L27/56 ,  A61K35/44 ,  A61L27/24 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  A61K35/33 ,  A61K35/34 ,  A61K35/39 ,  A61K35/407 ,  A61L27/20

Abstract: The present application relates to biomimetic three-dimensional (3D) scaffolds, constructs and methods of making the same. The three-dimensional scaffold can include a sacrificial internal cast and a durable external scaffold material, wherein the durable external scaffold material comprises a biocompatible material which completely surrounds the sacrificial internal cast and wherein the sacrificial internal cast be removed to yield a branching 3D network of hollow, vessel-like tubes that substantially mimics a native tissue or organ.

公开(公告)号：US20180214604A1

公开(公告)日：2018-08-02

申请号：US15938706

申请日：2018-03-28

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: A61L27/36 ,  A61L27/38 ,  A61K35/34 ,  A61K38/18 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/18 ,  C12N5/077 ,  C12N5/00 ,  A61L27/54 ,  A61L27/24 ,  A61K9/00

CPC classification number: A61L27/3604 ,  A61K9/0034 ,  A61K35/34 ,  A61K38/18 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/18 ,  A61L27/24 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/414 ,  A61L2400/06 ,  A61L2430/30 ,  C12N5/0062 ,  C12N5/0659 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/54 ,  A61K2300/00 ,  C08L67/04

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

公开(公告)号：US20140242125A1

公开(公告)日：2014-08-28

申请号：US14239416

申请日：2012-08-15

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: A61K35/34 ,  A61K47/34 ,  A61K45/06 ,  A61K47/42

CPC classification number: A61L27/3604 ,  A61K9/0034 ,  A61K35/34 ,  A61K38/18 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/18 ,  A61L27/24 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/414 ,  A61L2400/06 ,  A61L2430/30 ,  C12N5/0062 ,  C12N5/0659 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/54 ,  A61K2300/00 ,  C08L67/04

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

Abstract translation: 本发明涉及用于获得用于移植的均匀尺寸的肌纤维片段的方法和组合物。 这些肌纤维碎片能够重建成沿着原生肌肉定向的长纤维。 植入的肌肉细胞与天然血管和神经网络结合，如组织学和免疫组织化学所证实的。 本发明是特别有利的，因为可以从供体部位收获自体肌肉，将其加工并注射到手术室中的靶位点。 碎片肌肉纤维可以容易地集成在宿主内。

公开(公告)号：US11013828B2

公开(公告)日：2021-05-25

申请号：US15938706

申请日：2018-03-28

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: A61L27/36 ,  A61K38/18 ,  A61L27/18 ,  A61L27/24 ,  A61L27/38 ,  A61K35/34 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/54 ,  C12N5/00 ,  C12N5/077 ,  A61K9/00

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

公开(公告)号：US09962466B2

公开(公告)日：2018-05-08

申请号：US15159219

申请日：2016-05-19

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: C12N5/077 ,  A61L27/36 ,  A61K35/34 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61K38/18 ,  A61L27/18 ,  A61L27/24 ,  A61L27/38 ,  A61L27/54 ,  C12N5/00 ,  A61K9/00

CPC classification number: A61L27/3604 ,  A61K9/0034 ,  A61K35/34 ,  A61K38/18 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/18 ,  A61L27/24 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/414 ,  A61L2400/06 ,  A61L2430/30 ,  C12N5/0062 ,  C12N5/0659 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/54 ,  A61K2300/00 ,  C08L67/04

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

公开(公告)号：US20160296667A1

公开(公告)日：2016-10-13

申请号：US15159219

申请日：2016-05-19

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: A61L27/36 ,  A61L27/24 ,  C12N5/00 ,  A61L27/54 ,  C12N5/077 ,  A61L27/38 ,  A61L27/18

CPC classification number: A61L27/3604 ,  A61K9/0034 ,  A61K35/34 ,  A61K38/18 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/18 ,  A61L27/24 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/414 ,  A61L2400/06 ,  A61L2430/30 ,  C12N5/0062 ,  C12N5/0659 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/54 ,  A61K2300/00 ,  C08L67/04

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

Abstract translation: 本发明涉及用于获得用于移植的均匀尺寸的肌纤维片段的方法和组合物。 这些肌纤维碎片能够重建成沿着原生肌肉定向的长纤维。 植入的肌肉细胞与天然血管和神经网络结合，如组织学和免疫组织化学所证实的。 本发明是特别有利的，因为可以从供体部位收获自体肌肉，将其加工并注射到手术室中的靶位点。 碎片肌肉纤维可以容易地集成在宿主内。