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19 条记录 (耗时 0.03 秒)

    Dna synthesis promoters dna polymerase-associated factors and utilization thereof
    11.
    发明申请
    Dna synthesis promoters dna polymerase-associated factors and utilization thereof 有权
    Dna合成启动子dna聚合酶相关因子及其利用

    公开(公告)号：US20050069887A1

    公开(公告)日：2005-03-31

    申请号：US10495581

    申请日：2002-11-14

    申请人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C07K14/195 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C07K14/195 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2521/101

    摘要： A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy  (1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3. The present invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or bind to DNA polymerase.

    摘要翻译： 一种用于增强DNA合成的组合物，其包含在促进DNA合成反应中有效促进DNA合成的阴离子供给物质，特别是二羧酸的盐。 通过与阴离子供给物质一起使用至少一种选自二甲基亚砜和下式（1）表示的化合物的化合物，可以实现进一步增强的DNA合成促进效果：R 2 -CH 2 -NR <1> xHy（1）其中R 1是具有1至3个碳原子的烷基，R 2是选自以下（a）和（b）的取代基：（a）氧 和（b）由下式表示的基团其中R 4是甲基，氢或当与R 1一起形成吡咯烷环时，R 5是-CO 2 H或-SO 3 H，n是0至 2，x是1至3的整数，y是0至2的整数，条件是x加y等于3.本发明还提供了源自Thermococcus物种的热稳定DNA聚合酶相关因子，其具有活性 促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合。

    Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same
    16.
    发明申请
    Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same 审中-公开
    靶向基因破坏的方法，超嗜热细菌的基因组和使用其的基因组芯片

    公开(公告)号：US20060248617A1

    公开(公告)日：2006-11-02

    申请号：US10526324

    申请日：2003-08-29

    申请人： Tadayuki Imanaka ,  Haruyuki Atomi

    发明人： Tadayuki Imanaka ,  Haruyuki Atomi

    IPC分类号： G06F19/00 ,  C12Q1/68 ,  C07H21/02 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C07K14/195 ,  C12Q1/02 ,  G01N2333/195 ,  G01N2500/10

    摘要： It is intended to provide an efficient and sure gene targeting method embodied at an arbitrary position in the genome of an organism and a kit therefor. It is also intended to provide a method for targeted-disruption of an arbitrary gene in the genome of an organism which comprises: 1) the step of providing the whole sequencial data of the genome of the organism; 2) the step of selecting at least one arbitrary region in the sequence; 3) the step of providing a vector containing a sequence homologous with the region selected above and a marker gene; 4) the step of transforming the organism by the vector; and 5) the step of providing the organism under such conditions as allowing homologous recombination. Moreover, the genome of a hyperthermostable bacterium and its array are provided.

    摘要翻译： 旨在提供一种在生物体的基因组中任意位置实施的有效且可靠的基因靶向方法及其用于其的试剂盒。 还旨在提供一种用于靶向生物体基因组中任意基因的靶向破坏的方法，其包括：1）提供生物体基因组的整个序列数据的步骤; 2）选择序列中的至少一个任意区域的步骤; 3）提供含有与上述选择区域同源的序列的载体和标记基因的步骤; 4）通过载体转化生物体的步骤; 和5）在允许同源重组的条件下提供生物体的步骤。 此外，提供了超热稳定细菌的基因组及其阵列。