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    AUTOMATED BLOTTING USING SLIDING DEVICES
    11.
    发明申请
    AUTOMATED BLOTTING USING SLIDING DEVICES 有权
    使用滑动装置的自动吹瓶

    公开(公告)号：US20150253251A1

    公开(公告)日：2015-09-10

    申请号：US14642487

    申请日：2015-03-09

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong

    IPC: G01N21/76 ,  G01N21/03

    CPC classification number: B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/065 ,  C12Q1/6806 ,  G01N27/44717 ,  G01N27/44739 ,  G01N33/54366

    Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

    Abstract translation: 提供了设备，系统，方法和试剂盒，用于进行生物样品中分析物的分离，固定，印迹和/或检测。 在一些实施例中，装置由具有接触表面的两个固体基板构成。 这些装置包括从这些表面中的凹陷形成的多个通道。 凹陷可以在基板之间的界面上彼此对齐，并且通过相对于另一个移动或滑动一个基板来重新对准。 在一些实施例中，装置由三层固体基板构成。 器件中间层的分离通道首先用于分析物分离。 然后中间层可以相对于顶层和/或底层滑动，从而使分离通道与印迹膜对准。 然后可以使用顶层和底层中的电极将分析物转移到膜。

    Targeted delivery of reagents to spots on a planar support through patterned transfer sheets
    17.
    发明授权
    Targeted delivery of reagents to spots on a planar support through patterned transfer sheets 有权
    通过图案化转印片将试剂靶向递送到平面支架上的斑点

    公开(公告)号：US09594054B2

    公开(公告)日：2017-03-14

    申请号：US13950590

    申请日：2013-07-25

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong

    IPC: G01N27/447

    CPC classification number: G01N27/44739 ,  G01N27/44747 ,  G01N33/54306 ,  G01N33/54393

    Abstract: Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

    Abstract translation: 固定在平面载体上的离散位置上的分子物质如凝胶或印迹膜上的蛋白质条带或涂在膜上的点或点上的物质与通过多孔亲水转移片施加的结合试剂反应， 所述片材具有至少一个区域，所述至少一个区域被阻挡物侧向界定，所述粘合剂保留在所述边界区域内。 如果分子种类存在于支持体上，则边界区域直接放置在平面支撑体上的分子种类预期驻留的区域上。 然后将结合试剂输送到载体中以接触物种。 因此，以提高的效率实现了结合试剂的靶向递送。

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