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    INSTRUMENT FOR INDEPENDENT ELECTROTRANSFER IN MULTIPLE CASSETTES
    3.
    发明申请
    INSTRUMENT FOR INDEPENDENT ELECTROTRANSFER IN MULTIPLE CASSETTES 有权
    用于多个CASSETTES中独立电极的仪器

    公开(公告)号：US20160041121A1

    公开(公告)日：2016-02-11

    申请号：US14920646

    申请日：2015-10-22

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Matthew Latham ,  William Strong ,  Nikolas Chmiel ,  Jeff Xu ,  Robert Iovanni

    IPC: G01N27/447

    CPC classification number: G01N27/44739 ,  B01D57/02 ,  G01N27/44782

    Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.

    Abstract translation: 电传递在接收电印箱的仪器中进行，并且包含集成的电源，控制和显示器，其允许用户通过壳体内的电接触单独地监测和控制多个盒中的每一个，从而与相应的电触点 在盒子上

    DIP-STICK WESTERN BLOT
    4.
    发明申请
    DIP-STICK WESTERN BLOT 审中-公开

    公开(公告)号：US20150260681A1

    公开(公告)日：2015-09-17

    申请号：US14644527

    申请日：2015-03-11

    Applicant: BIO-RAD LABORATORIES, INC.

    Inventor： William Strong

    IPC: G01N27/447 ,  G01N33/543

    CPC classification number: G01N27/44739 ,  G01N30/90 ,  G01N33/54386

    Abstract: Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick.

    Abstract translation: 提供了方法，试剂盒和系统，用于使用骰子分离，固定和/或检测一种或多种样品的分析物。 “定尺”是可以从分离介质中嵌入并随后除去的物体，并且当物体嵌入分离介质中时可以固定分析物。 分离介质的实例包括任何形式的电泳凝胶和用于柱色谱法的固定相。 本方法的实施方案包括将样品应用于分离介质; 沿着分离轴分离分离介质中样品的分析物; 将分析物固定在嵌入分离介质中的试纸上; 从分离介质中取出量油尺; 并检测固定在去除的量油尺上的分析物。

    Mini-gel comb
    6.
    发明授权
    Mini-gel comb 有权

    公开(公告)号：US10101296B2

    公开(公告)日：2018-10-16

    申请号：US14558541

    申请日：2014-12-02

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： William Strong

    IPC: G01N27/00 ,  G01N27/447

    Abstract: Molds for casting and retaining electrophoresis gel strips are provided, along with methods, kits, and systems for performing electrophoresis, electroelution, and/or electroblotting using these molds.

    Protein detection using modified cyclodextrins
    8.
    发明授权
    Protein detection using modified cyclodextrins 有权
    使用改性环糊精的蛋白质检测

    公开(公告)号：US09285359B2

    公开(公告)日：2016-03-15

    申请号：US14455609

    申请日：2014-08-08

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong ,  Lee Olech ,  Thomas Berkelman ,  Christopher Belisle

    IPC: G01N33/53 ,  G01N33/58 ,  G01N33/68 ,  G01N27/447

    CPC classification number: G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

    Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.

    Abstract translation: 提供了一种使用共价连接至少一个标签的环糊精来检测蛋白质的方法。 环糊精可以通过在其疏水腔中螯合蛋白质的芳香族氨基酸侧链而与蛋白质结合。 将蛋白质与环糊精接触后，可以直接检测标记物，或者可以与试剂进行化学相互作用以形成可检测的产物。 标记可以包括吲哚部分，其可以在暴露于紫外光时与卤素取代的有机化合物反应，从而变成荧光。 或者，标记可以包括生物素部分，其可以结合结合配偶体，例如抗生物素蛋白或其变体，以形成可检测的分子复合物。 标记的环糊精可用于本方法中以检测电泳凝胶或印迹膜上的目的蛋白质。 蛋白质，特别是色氨酸的芳香族氨基酸残基由于环糊精的螯合而保护免受化学修饰，使得这些方法与需要完整蛋白质的下游应用相容。 本文还提供了用于检测蛋白质的组合物，试剂盒和电泳凝胶。

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