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    Protein detection using modified cyclodextrins
    2.
    发明授权
    Protein detection using modified cyclodextrins 有权
    使用改性环糊精的蛋白质检测

    公开(公告)号：US09285359B2

    公开(公告)日：2016-03-15

    申请号：US14455609

    申请日：2014-08-08

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong ,  Lee Olech ,  Thomas Berkelman ,  Christopher Belisle

    IPC: G01N33/53 ,  G01N33/58 ,  G01N33/68 ,  G01N27/447

    CPC classification number: G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

    Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.

    Abstract translation: 提供了一种使用共价连接至少一个标签的环糊精来检测蛋白质的方法。 环糊精可以通过在其疏水腔中螯合蛋白质的芳香族氨基酸侧链而与蛋白质结合。 将蛋白质与环糊精接触后，可以直接检测标记物，或者可以与试剂进行化学相互作用以形成可检测的产物。 标记可以包括吲哚部分，其可以在暴露于紫外光时与卤素取代的有机化合物反应，从而变成荧光。 或者，标记可以包括生物素部分，其可以结合结合配偶体，例如抗生物素蛋白或其变体，以形成可检测的分子复合物。 标记的环糊精可用于本方法中以检测电泳凝胶或印迹膜上的目的蛋白质。 蛋白质，特别是色氨酸的芳香族氨基酸残基由于环糊精的螯合而保护免受化学修饰，使得这些方法与需要完整蛋白质的下游应用相容。 本文还提供了用于检测蛋白质的组合物，试剂盒和电泳凝胶。

    TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS
    3.
    发明申请
    TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS 有权
    通过图案转让表对平面图支持的试剂进行指定的交付

    公开(公告)号：US20140027284A1

    公开(公告)日：2014-01-30

    申请号：US13950590

    申请日：2013-07-25

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong

    IPC: G01N27/447

    CPC classification number: G01N27/44739 ,  G01N27/44747 ,  G01N33/54306 ,  G01N33/54393

    Abstract: Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

    Abstract translation: 固定在平面载体上的离散位置上的分子物质如凝胶或印迹膜上的蛋白质条带或涂在膜上的点或点上的物质与通过多孔亲水转移片施加的结合试剂反应， 所述片材具有至少一个区域，所述至少一个区域被阻挡物侧向界定，所述粘合剂保留在所述边界区域内。 如果分子种类存在于支持体上，则边界区域直接放置在平面支撑体上的分子种类预期驻留的区域上。 然后将结合试剂输送到载体中以接触物种。 因此，以提高的效率实现了结合试剂的靶向递送。

    DRY PROTEIN TRANSFER
    10.
    发明申请
    DRY PROTEIN TRANSFER 有权
    干蛋白转移

    公开(公告)号：US20160231272A1

    公开(公告)日：2016-08-11

    申请号：US15019792

    申请日：2016-02-09

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  Steve Swihart

    IPC: G01N27/447

    CPC classification number: G01N27/44739

    Abstract: Kits, systems, and methods are provided for transferring biological macromolecules from an electrophoresis slab gel to a blotting membrane using conductive polymer electrodes.

    Abstract translation: 提供了使用导电聚合物电极将生物大分子从电泳板凝胶转移到印迹膜的试剂盒，系统和方法。

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