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公开(公告)号：US11590486B2
公开(公告)日：2023-02-28
申请号：US17008212
申请日：2020-08-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
Abstract: A method for manufacturing a mixed-mode chromatography medium is provided. The method can include, for example, oxidizing diol groups on diol-functionalized solid particles having pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter and having a diol density of from about 200 to about 300 μmol/mL to aldehyde groups, thereby converting said diol-functionalized solid particles to aldehyde-functionalized solid particles; and coupling amine-functionalized ligands to said aldehyde-functionalized solid particles, said amine-functionalized ligands comprising an amine-substituted hydrophobic group joined to an acid moiety selected from the group consisting of a carboxyl group and a sulfo group.
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公开(公告)号：US10119934B2
公开(公告)日：2018-11-06
申请号：US15272257
申请日：2016-09-21
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: G01N27/447 , B01D57/02 , C08F220/56
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
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公开(公告)号：US09309282B2
公开(公告)日：2016-04-12
申请号：US13654574
申请日：2012-10-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
CPC classification number: B01J39/26 , C07K1/165 , C07K1/22 , C07K16/065
Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.
Abstract translation: 蛋白质通过将配体与阳离子交换和疏水功能连接到大孔载体基质形成的混合模式色谱系统来纯化，配体和载体基质之间的唯一连接是具有不超过三个 疏水基团和载体基质之间的原子。
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公开(公告)号：US20170067852A1
公开(公告)日：2017-03-09
申请号：US15272257
申请日：2016-09-21
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: G01N27/447 , C08F220/56 , B01D57/02
CPC classification number: G01N27/44747 , B01D57/02 , C08F220/56 , C08F222/385
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。
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公开(公告)号：US09034652B2
公开(公告)日：2015-05-19
申请号：US14205182
申请日：2014-03-11
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Christopher Belisle , Lee Olech , Thomas Berkelman , Praveena Garimella
IPC: G01N33/68
CPC classification number: G01N33/6839 , G01N33/68 , Y10T436/10 , Y10T436/107497
Abstract: Colloidal formulation for staining proteins and methods of their use are provided.
Abstract translation: 提供用于染色蛋白质的胶体制剂及其使用方法。
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Patent Agency Ranking
公开(公告)号：US10792654B2
公开(公告)日：2020-10-06
申请号：US15063235
申请日：2016-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand comprising benzamidoacetic acid to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between a phenyl ring and the support matrix.
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公开(公告)号：US20150044705A1
公开(公告)日：2015-02-12
申请号：US14455609
申请日：2014-08-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Clayton T. McKee , William Strong , Lee Olech , Thomas Berkelman , Christopher Belisle
IPC: G01N33/53 , G01N27/447
CPC classification number: G01N33/5306 , G01N27/44747 , G01N33/582 , G01N33/6803 , G01N2400/18
Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.
Abstract translation: 提供了一种使用共价连接至少一个标签的环糊精来检测蛋白质的方法。 环糊精可以通过在其疏水腔中螯合蛋白质的芳香族氨基酸侧链而与蛋白质结合。 将蛋白质与环糊精接触后，可以直接检测标记物，或者可以与试剂进行化学相互作用以形成可检测的产物。 标记可以包括吲哚部分，其可以在暴露于紫外光时与卤素取代的有机化合物反应，从而变成荧光。 或者，标记可以包括生物素部分，其可以结合结合配偶体，例如抗生物素蛋白或其变体，以形成可检测的分子复合物。 标记的环糊精可用于本方法中以检测电泳凝胶或印迹膜上的目的蛋白质。 蛋白质，特别是色氨酸的芳香族氨基酸残基由于环糊精的螯合而保护免受化学修饰，使得这些方法与需要完整蛋白质的下游应用相容。 本文还提供了用于检测蛋白质的组合物，试剂盒和电泳凝胶。
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公开(公告)号：US20140273251A1
公开(公告)日：2014-09-18
申请号：US14205182
申请日：2014-03-11
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Christopher Belisle , Lee Olech , Thomas Berkelman , Praveena Garimella
IPC: G01N33/68
CPC classification number: G01N33/6839 , G01N33/68 , Y10T436/10 , Y10T436/107497
Abstract: Colloidal formulation for staining proteins and methods of their use are provided.
Abstract translation: 提供用于染色蛋白质的胶体制剂及其使用方法。
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公开(公告)号：US20130102761A1
公开(公告)日：2013-04-25
申请号：US13654574
申请日：2012-10-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
IPC: C07K1/22 , C08F271/02 , C07K16/00
CPC classification number: B01J39/26 , C07K1/165 , C07K1/22 , C07K16/065
Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.
Abstract translation: 蛋白质通过将配体与阳离子交换和疏水功能连接到大孔载体基质形成的混合模式色谱系统来纯化，配体和载体基质之间的唯一连接是具有不超过三个 疏水基团和载体基质之间的原子。
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公开(公告)号：US09486741B2
公开(公告)日：2016-11-08
申请号：US13934936
申请日：2013-07-03
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: G01N27/447 , B01D57/02 , C08F220/56
CPC classification number: G01N27/44747 , B01D57/02 , C08F220/56 , C08F222/385
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
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