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公开(公告)号：US09309282B2
公开(公告)日：2016-04-12
申请号：US13654574
申请日：2012-10-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
CPC classification number: B01J39/26 , C07K1/165 , C07K1/22 , C07K16/065
Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.
Abstract translation: 蛋白质通过将配体与阳离子交换和疏水功能连接到大孔载体基质形成的混合模式色谱系统来纯化，配体和载体基质之间的唯一连接是具有不超过三个 疏水基团和载体基质之间的原子。
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公开(公告)号：US10682640B2
公开(公告)日：2020-06-16
申请号：US15581569
申请日：2017-04-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Russell Frost
IPC: B01J41/20 , C07K1/16 , B01J20/22 , B01D15/30 , B01J20/281 , B01D15/26 , C07K1/14 , B01D15/32 , B01D15/36 , B01D15/38 , B01J20/286 , B01J20/289 , B01J20/32 , B01J41/09 , B01J20/26 , B01J20/28 , B01J20/288 , B01J41/04 , C07K16/00
Abstract: Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)-R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.
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Patent Agency Ranking
公开(公告)号：US20150258539A1
公开(公告)日：2015-09-17
申请号：US14657701
申请日：2015-03-13
Inventor： Steven Cramer , James Woo , Hong Chen , Jiali Liao , Russell Frost
Abstract: Mixed mode ligands, as well their use for purifying proteins, are provided.
Abstract translation: 提供了混合模式配体，以及它们用于纯化蛋白质的用途。
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公开(公告)号：US11590486B2
公开(公告)日：2023-02-28
申请号：US17008212
申请日：2020-08-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
Abstract: A method for manufacturing a mixed-mode chromatography medium is provided. The method can include, for example, oxidizing diol groups on diol-functionalized solid particles having pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter and having a diol density of from about 200 to about 300 μmol/mL to aldehyde groups, thereby converting said diol-functionalized solid particles to aldehyde-functionalized solid particles; and coupling amine-functionalized ligands to said aldehyde-functionalized solid particles, said amine-functionalized ligands comprising an amine-substituted hydrophobic group joined to an acid moiety selected from the group consisting of a carboxyl group and a sulfo group.
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公开(公告)号：US10583429B2
公开(公告)日：2020-03-10
申请号：US14657701
申请日：2015-03-13
Inventor： Steven Cramer , James Woo , Hong Chen , Jiali Liao , Russell Frost
Abstract: The present disclosure is directed to mixed mode chromatography media comprising a ligand directly attached to a solid support. In some aspects, the ligand has a chemical formula of The mixed mode chromatography media is useful for binding and purifying proteins from a solution.
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公开(公告)号：US10792654B2
公开(公告)日：2020-10-06
申请号：US15063235
申请日：2016-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand comprising benzamidoacetic acid to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between a phenyl ring and the support matrix.
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公开(公告)号：US20130102761A1
公开(公告)日：2013-04-25
申请号：US13654574
申请日：2012-10-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Jiali Liao , Lee Olech , Hong Chen , Xuemei He , Russell Frost
IPC: C07K1/22 , C08F271/02 , C07K16/00
CPC classification number: B01J39/26 , C07K1/165 , C07K1/22 , C07K16/065
Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.
Abstract translation: 蛋白质通过将配体与阳离子交换和疏水功能连接到大孔载体基质形成的混合模式色谱系统来纯化，配体和载体基质之间的唯一连接是具有不超过三个 疏水基团和载体基质之间的原子。
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