-
公开(公告)号：US20240177309A1
公开(公告)日：2024-05-30
申请号：US18519226
申请日：2023-11-27
发明人： Manuel AMTHOR , Daniel HAASE , Ralf WOLLESCHENSKY
IPC分类号： G06T7/00
CPC分类号： G06T7/0012 , G06T2207/10056 , G06T2207/10064 , G06T2207/20081
摘要： A method for preparing data for identifying analytes by coloring one or more analytes with markers in multiple coloring rounds, the markers in each case being specific for a certain set of analytes, detecting multiple markers using a camera, which for each coloring round generates at least one image that contains multiple pixels and includes colored signals and uncolored signals, a colored signal being a pixel containing color information of a marker, and an uncolored signal being a pixel containing color information that is not based on a marker, and storing the images of the particular coloring rounds for evaluating the color information, a data point in each case including one or more contiguous pixels in the images of the multiple coloring rounds, which are assigned to the same location in a sample, wherein
each of the data points is assessed, based on the color information of at least the present image, for whether it may be a candidate data point, i.e., that it may contain colored signals and may thus encode an analyte, and
when the color information is stored, the color information of the data points of the images which are reliably not a candidate data point, based on the assessment, is eliminated.-
公开(公告)号：US11988824B2
公开(公告)日：2024-05-21
申请号：US17696744
申请日：2022-03-16
发明人： Alexander Gaiduk , Ilja Karanin , Pavlos Iliopoulos , Jin Young Sohn , Gyoungil Cho , Cheong Soo Seo
IPC分类号： G02B21/36 , H04N25/531 , H04N25/705
CPC分类号： G02B21/361 , H04N25/531 , H04N25/705
摘要： The present invention concerns a method for producing a microscopic image with an extended depth of field by means of a microscope. The microscope comprises an images sensor that comprises pixels that are arranged as a matrix that is formed by lines. In a step of the method, a plurality of microscopic frames of a specimen is acquired while a focus position (z) is changed. The microscopic frames are acquired line by line. The focus position (z) is changed over a course of acquiring individuals of the microscopic frames. In a further step, parts of individuals of the acquired lines are identified. These parts sharply image the specimen. The identified parts of the lines are composed in order to form a microscopic image of the specimen with an extended depth of field. Furthermore, the present invention concerns a microscope.
-
公开(公告)号：US11977216B2
公开(公告)日：2024-05-07
申请号：US17766098
申请日：2020-10-12
发明人： Daniel Schwedt , Tiemo Anhut
CPC分类号： G02B21/367 , G01J1/32 , G01J1/44 , G01J2001/442
摘要： A light microscope and a method for capturing images with a light microscope includes guiding illumination light to a sample; guiding detection light from the sample to a plurality of photon-counting sensor elements, which each successively capture a plurality of photon counts; forming a plurality of photon count distributions to be analyzed and at least one reference photon count distribution from the photon counts; calculating a similarity between each photon count distribution to be analyzed and the reference photon count distribution; and identifying sensor elements as overdriven as a function of the calculated similarity of the corresponding photon count distribution(s) to be analyzed.
-
公开(公告)号：US11966036B2
公开(公告)日：2024-04-23
申请号：US17296623
申请日：2019-11-07
发明人： Tiemo Anhut , Daniel Schwedt
CPC分类号： G02B21/0032 , G01N21/6458 , G02B21/0076 , G02B21/008 , G02B21/04
摘要： A light microscope having a scanner for scanning a sample with illuminating light and a light detector for measuring sample light. A microlens array having a plurality of microlenses is arranged in front of the light detector in the region of a pupil plane. The light detector respectively has a plurality of detector elements behind each microlens and has a complete readout frequency of at least 100 kHz. By means of the detector elements arranged behind the respective microlens, a wavefront datum regarding the sample light is determined. Moreover, a sample point signal is computed from signals of the detector elements. Illuminating light is successively deflected onto different sample points with the scanner and corresponding sample point signals are captured, wherein, for at least some of the different sample points, a corresponding wavefront datum is also determined. The determined wavefront data can be taken into account in a calculation of a sample image from the plurality of sample point signals.
-
公开(公告)号：US11933956B2
公开(公告)日：2024-03-19
申请号：US17247210
申请日：2020-12-03
发明人： Jörg Siebenmorgen , Ralf Netz
CPC分类号： G02B21/08 , G02B21/16 , G02B27/425 , G02B27/4294
摘要： An illumination apparatus for illuminating a sample plane and a sample that is optionally arranged therein along an illumination beam path includes: a first light source (for outputting light of at least one first wavelength (λphoto), a second light source for outputting light of at least one second wavelength (λexc), and a diffraction grating in the illumination beam path between the first and second light sources and the sample plane. Light of the first wavelength (λphoto) is not diffracted by the diffraction grating, and light of the second wavelength (λexc) is diffracted due to the effect of the diffraction grating. The illumination apparatus can be used in a microscope.
-
公开(公告)号：US20240053594A1
公开(公告)日：2024-02-15
申请号：US18231988
申请日：2023-08-09
发明人： Joerg Steinert , Tiemo Anhut , Daniel Schwedt , Wiebke Hilbert , Gerd Heisse
CPC分类号： G02B21/008 , G02B21/361 , G02B21/0032
摘要： Microscopy and a device for microscopy where with a light field arrangement comprising a multi-lens array and a camera having at least one camera sensor, image data sets which each contain at least one partial image of a sample are successively recorded. To increase a number of the image data sets recordable per unit time, a) measurement data only from a first number of pixels less than a total number of pixels of the at least one camera sensor are read out; b) measurement data only from a second number of pixels less than the total number of pixels or the first number of pixels read are processed further; and/or c) for some or all of the total number of pixels or the first number of pixels read, measurement data are processed further with a bit depth which is less than a maximum possible bit depth.
-
17.发明授权公开(公告)号：US11900573B2
公开(公告)日：2024-02-13
申请号：US17385880
申请日：2021-07-26
发明人： Manuel Amthor , Daniel Haase
CPC分类号： G06T5/009 , G06T5/40 , G06T5/50 , G06T2207/10056 , G06T2207/10152
摘要： The invention relates to a method and device for generating an overview contrast image of a sample carrier in a microscope, which images a sample arranged on the sample carrier. The sample carrier on which the sample is located is illuminated in transmitted light from a two-dimensional illumination array, which has individually switchable single light sources and illuminates the sample volume in transmitted light, and a processing unit, which activates and reads out the camera in order to record multiple different raw overview images of the sample carrier.
-
公开(公告)号：US20230420224A1
公开(公告)日：2023-12-28
申请号：US18230722
申请日：2023-08-07
发明人： Andreas Schmaunz
IPC分类号： H01J37/32 , H01J37/145 , H01J37/244 , H01J37/28 , H01J37/304
CPC分类号： H01J37/32449 , H01J37/145 , H01J37/244 , H01J37/28 , H01J37/304 , H01J2237/006 , H01J2237/2065
摘要： Operating a gas feed device for a particle beam apparatus includes predetermining a flow rate of a precursor through an outlet of a precursor reservoir containing the precursor to be fed onto an object, loading a temperature of the precursor reservoir, the temperature being associated with the predetermined flow rate, from a database into a control unit, setting a temperature of the precursor reservoir to the temperature loaded from the database using a temperature setting unit, and determining at least one functional parameter of the precursor reservoir depending on the flow rate and the temperature, loaded from the database, using the control unit and informing a user of the gas feed device about the determined functional parameter. Informing the user of the gas feed device about the functional parameter may include displaying the functional parameter on a display unit, outputting an optical signal, or outputting an acoustic signal.
-
公开(公告)号：US20230418042A1
公开(公告)日：2023-12-28
申请号：US18213374
申请日：2023-06-23
发明人： Manuel Amthor , Daniel Haase , Thomas Ohrt
CPC分类号： G02B21/367 , G06T7/11 , G06T7/90 , G06T2207/10056
摘要： For the color correction of microscope images, an object that corresponds to a predetermined object type of a known color is localized in a microscope image using an image processing program. Based on an image region of the localized object, a color correction is determined with which a color of the localized object in the image region is brought into accordance with the known color. The color correction is applied to at least one section of the microscope image or of another microscope image or is used in a capture of a further microscope image.
-
20.发明公开公开(公告)号：US20230384575A1
公开(公告)日：2023-11-30
申请号：US18203208
申请日：2023-05-30
摘要： A distance measuring device in microscopy contains a sample stage for arranging a sample carrier in a sample plane, and at least one, preferably at least two illumination sources for providing measurement radiation, which is reflected at least proportionally at a surface of the sample carrier. The device also contains a detection optical unit for capturing an overview image and occurring reflections at the sample carrier present in the sample plane; a detector disposed downstream of the detection optical unit and serving for the spatially resolved capture of image data of the sample carrier and of occurring reflections; and an evaluation device for ascertaining at least a distance of the surface at at least one location of the sample carrier. The illumination sources emit the measurement radiation in each case at a divergent emission angle. A corresponding method can be used for ascertaining a spatial orientation of a sample carrier.
-
-
-
-
-
-
-
-
-
搜索结果
988 条记录 (耗时 0.032 秒)
