公开(公告)号：US20020094562A1

公开(公告)日：2002-07-18

申请号：US09771383

申请日：2001-01-25

发明人： Kevin Delos Parris ,  William Stuart Somers ,  Amy Szepui Tam ,  Laura Long Lin ,  Mark Lloyd Stahl

IPC分类号： C12N009/16 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC分类号： C12N9/1288

摘要： This invention is directed to Acyl Carrier Protein Synthase (ACPS) crystals and crystals of Acyl Carrier Protein Synthase-Coenzyme A (ACPS-CoA) complex, and to the use of these crystals to determine the three dimensional structure of ACPS. This invention is further directed to the use of rational drug design methods to identify agents that may interact with active sites of ACPS and ACPS-CoA complex, and to the testing of these agents to identify agents that may inhibit ACPS and/or ACPS-CoA complex activity.

摘要翻译： 本发明涉及酰基载体蛋白合成酶（ACPS）晶体和酰基载体蛋白合成酶辅酶A（ACPS-CoA）复合物的晶体，以及这些晶体的用途来确定ACPS的三维结构。 本发明进一步涉及使用合理的药物设计方法来鉴定可与ACPS和ACPS-CoA复合物的活性位点相互作用的试剂，以及这些试剂的测试以鉴定可抑制ACPS和/或ACPS-CoA的药剂 复杂的活动。

公开(公告)号：US06258566B1

公开(公告)日：2001-07-10

申请号：US09422073

申请日：1999-10-21

申请人： Philip J. Barr ,  Daniel V. Santi ,  Gary W. Ashley ,  Rainer Ziermann

发明人： Philip J. Barr ,  Daniel V. Santi ,  Gary W. Ashley ,  Rainer Ziermann

IPC分类号： C12P1962

CPC分类号： C12N9/1288 ,  A01K2217/05 ,  C07K2319/00 ,  C12N9/00 ,  C12N9/1025 ,  C12N15/52 ,  C12N15/70 ,  C12N15/76 ,  C12N15/81 ,  C12P7/26 ,  C12P7/42 ,  C12P17/162 ,  C12P19/62

摘要： Hybrid and novel polyketide synthases (PKSs) and polyketides are produced by use of a multiple vector system. The combinatorial possibilities offered by placing the various catalytic activities of PKS systems on separate vectors permits the construction of improved libraries of PKS and polyketides. In addition, polyketides can be produced in hosts that ordinarily do not produce polyketides by supplying, along with an expression system for the desired PKS, an expression system for holo acyl carrier protein (ACP) synthase.

摘要翻译： 通过使用多重载体系统产生杂合和新型聚酮化合物合酶（PKS）和聚酮化合物。 通过将PKS系统的各种催化活性放置在单独的载体上提供的组合可能性允许构建改进的PKS和聚酮化合物库。 此外，聚酮化合物可以在通常不通过供应所需的PKS的表达系统，全酰酰基载体蛋白（ACP）合酶的表达系统）的情况下产生聚酮化合物的宿主中生产。

公开(公告)号：US20180135066A9

公开(公告)日：2018-05-17

申请号：US14235435

申请日：2012-07-26

申请人： Terence A. Walsh ,  Daniel Gachotte ,  Ann Owens Merlo ,  Dayakar Reddy Pareddy ,  James Metz ,  Scott Bevan ,  Jerry Kuner

发明人： Terence A. Walsh ,  Daniel Gachotte ,  Ann Owens Merlo ,  Dayakar Reddy Pareddy ,  James Metz ,  Scott Bevan ,  Jerry Kuner

IPC分类号： C12N15/82 ,  C12P7/64

CPC分类号： C12N15/8247 ,  A01H5/10 ,  A23L11/07 ,  A23V2002/00 ,  A61K31/202 ,  C12N9/1029 ,  C12N9/1288 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y203/01085

摘要： The invention provides recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US20180016585A1

公开(公告)日：2018-01-18

申请号：US15523829

申请日：2015-05-18

申请人： Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

发明人： Yong Wang ,  Zhiqiang Xiong ,  Shujie Song ,  Qiaoxia Liu ,  Jianfeng Wang

IPC分类号： C12N15/70 ,  C12Q1/18 ,  C12N15/09 ,  C12R1/465 ,  C07H17/08

CPC分类号： C12N15/70 ,  C07H17/08 ,  C12N9/1288 ,  C12N15/09 ,  C12N15/113 ,  C12N2320/12 ,  C12N2330/31 ,  C12N2330/50 ,  C12P17/08 ,  C12P19/62 ,  C12Q1/18 ,  C12R1/465

摘要： The present invention relates to a method for improving the heterologous synthesis of a polyketide by E. coli and use thereof. The yield of the polyketide heterologously synthesized by E. coli is significantly increased by attenuating the expression of seventy-two genes, such as sucC and talB, in a host strain, wherein the highest yield increase rate can reach 60% or more. Currently, erythromycin is the most clear model compound in the study on the biosynthesis of polyketids. The production strain of the present invention enables massive accumulation of 6-deoxyerythronolide (6-dEB), an erythromycin precursor, in the fermentation process, laying the foundation for the industrial production of the heterologous synthesis of erythromycin by E. coli.

公开(公告)号：US20180010137A1

公开(公告)日：2018-01-11

申请号：US15650285

申请日：2017-07-14

申请人： REG Life Sciences, LLC

发明人： Derek L. GREENFIELD ,  Andreas W. Schirmer ,  Elizabeth J. CLARKE ,  Eli S. GROBAN ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC分类号： C12N15/70 ,  C12P7/64 ,  C12P7/04 ,  C12N9/02 ,  C12N9/90 ,  C12N9/88 ,  C12N9/12 ,  C12N9/10 ,  C12N9/04 ,  C12N9/00

CPC分类号： C12N15/70 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y101/011 ,  C12Y103/01009 ,  C12Y103/0101 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y207/08007 ,  C12Y402/01059 ,  C12Y503/03014 ,  C12Y604/01002

摘要： The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.

公开(公告)号：US20170233750A1

公开(公告)日：2017-08-17

申请号：US15391059

申请日：2016-12-27

申请人： Gene Bridges GmbH

发明人： Youming Zhang ,  Jun Fu ,  Xiaoying Bian ,  Adrian Francis Stewart ,  Rolf Muller

IPC分类号： C12N15/70 ,  A61K35/74 ,  C12N15/52 ,  C07D498/22 ,  C07K7/64 ,  C07G99/00 ,  C07K5/078 ,  C12N9/12 ,  C07D493/04

CPC分类号： C12N15/70 ,  A61K35/74 ,  A61K2035/11 ,  C07D245/02 ,  C07D417/06 ,  C07D493/04 ,  C07D498/22 ,  C07G17/00 ,  C07K5/06139 ,  C07K7/64 ,  C12N9/1288 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12N15/74 ,  C12P17/10 ,  C12P17/181 ,  C12Y207/08007 ,  Y02P20/52

摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.

公开(公告)号：US20170218401A1

公开(公告)日：2017-08-03

申请号：US15421861

申请日：2017-02-01

申请人： The University of Manchester

发明人： Nigel Scrutton ,  Patrik Jones ,  Navya Menon

IPC分类号： C12P5/02 ,  C12N9/12 ,  C12N15/52 ,  C12N9/16 ,  C12N9/88 ,  C12N9/04 ,  C12N9/10 ,  C12N9/02

CPC分类号： C12P5/02 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/16 ,  C12Y101/01001 ,  C12Y101/01021 ,  C12Y101/01157 ,  C12Y102/99006 ,  C12Y103/01038 ,  C12Y203/01009 ,  C12Y203/01194 ,  C12Y207/08007 ,  C12Y301/0202 ,  C12Y401/99005 ,  C12Y402/01055

摘要： Provided are genetically engineered microorganism that catalyze the synthesis of propane and/or butanol from a suitable substrate such as glucose. Also provided are methods of engineering said genetically engineered microorganism and methods of producing propane and/or butanol using the genetically engineered microorganism.

公开(公告)号：US09719117B2

公开(公告)日：2017-08-01

申请号：US14132473

申请日：2013-12-18

申请人： Steffen Schaffer ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

发明人： Steffen Schaffer ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

IPC分类号： C12P13/00 ,  C12N9/12 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10

CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/1288 ,  C12P13/005 ,  Y02P20/582

摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

公开(公告)号：US09677054B2

公开(公告)日：2017-06-13

申请号：US14977349

申请日：2015-12-21

申请人： BATTELLE ENERGY ALLIANCE, LLC.

发明人： David N Thompson ,  William A Apel ,  Vicki S Thompson ,  David W Reed ,  Jeffrey A Lacey

IPC分类号： C12P21/00 ,  C12N15/11 ,  C12N15/00 ,  C12N9/10 ,  C12N9/12

CPC分类号： C12N9/1051 ,  C12N9/1048 ,  C12N9/1288 ,  C12P21/00 ,  C12P21/005 ,  C12Y204/00 ,  C12Y204/01083 ,  C12Y207/08

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for glycosylating and/or post-translationally modifying proteins using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

公开(公告)号：US20160362696A1

公开(公告)日：2016-12-15

申请号：US15116600

申请日：2015-02-06

申请人： BASF SE

发明人： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC分类号： C12N15/52 ,  C12N15/74 ,  C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C12N9/12

CPC分类号： C12N15/52 ,  C12N1/02 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01 ,  C12Y203/01054 ,  C12Y207/08031

摘要： The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

摘要翻译： 本发明涉及与其野生型相比具有由wcaJ基因编码的酶的活性降低的修饰微生物。 本发明还涉及有机化合物的制造方法和改性微生物的用途。