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    Modified photosynthetic microorganisms for producing lipids
    92.
    发明授权
    Modified photosynthetic microorganisms for producing lipids 有权
    用于生产脂质的改良光合微生物

    公开(公告)号：US09523096B2

    公开(公告)日：2016-12-20

    申请号：US13995925

    申请日：2011-12-19

    申请人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    发明人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    IPC分类号： C12N1/00 ,  C12N1/21 ,  C12N15/82 ,  C07K14/195 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/00 ,  C12P7/64

    CPC分类号： C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

    摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

    摘要翻译： 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。

    Method for producing phenol from renewable resources by fermentation
    94.
    发明授权
    Method for producing phenol from renewable resources by fermentation 有权
    通过发酵从可再生资源生产苯酚的方法

    公开(公告)号：US09512449B2

    公开(公告)日：2016-12-06

    申请号：US14442468

    申请日：2013-11-13

    申请人： BAYER TECHNOLOGY SERVICES GMBH ,  BAYER MATERIALSCIENCE AG

    发明人： Jorgen Magnus

    IPC分类号： C12N9/88 ,  C12P7/22 ,  C07K14/245 ,  C12N9/90 ,  C12N15/52

    CPC分类号： C12P7/22 ,  C07K14/245 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y401/01061 ,  C12Y401/0304 ,  C12Y402/01051 ,  C12Y504/99005

    摘要： The invention relates to a method of generating a recombinant host strain for producing phenol, comprising the steps of a) providing a host comprising chorismate, b) transforming the host with a first nucleic acid sequence comprising ubiC (SEQ ID NO: 1) encoding chorismate lyase that converts chorismate to 4-hydroxybenzoate, and c) transforming the host with a second nucleic acid sequence encoding an oxygen-tolerant 4-hydroxybenzoate decarboxylase that converts 4-hydroxybenzoate to phenol, thereby generating a recombinant host that is capable of producing phenol under aerobic conditions, wherein step b) and step c) are carried out simultaneously or sequentially. The invention also provides the recombinant host strain for producing phenol obtainable by the aforementioned method, as well as a method of producing phenol in the recombinant host strain.

    摘要翻译： 本发明涉及产生用于产生苯酚的重组宿主菌株的方法，其包括以下步骤：a）提供包含顺阳离子的宿主，b）用包含编号绒螯蟹的ubiC（SEQ ID NO：1）的第一核酸序列转化宿主 将佐剂酸转化为4-羟基苯甲酸酯的裂解酶，以及c）用编码耐受4-羟基苯甲酸甲酯至苯酚的耐氧性4-羟基苯甲酸脱羧酶的第二核酸序列转化宿主，从而产生能够产生苯酚的重组宿主 其中步骤b）和步骤c）同时或依次进行。 本发明还提供了通过上述方法获得的用于生产苯酚的重组宿主菌株，以及在重组宿主菌株中生产苯酚的方法。