公开(公告)号：US11913029B2

公开(公告)日：2024-02-27

申请号：US18061811

申请日：2022-12-05

Applicant: Cellino Biotech, Inc.

Inventor： Matthias Wagner ,  Suvi Aivio ,  Mariangela Amenduni ,  Catherine Pilsmaker ,  Arnaldo Pereira ,  Ananya Zutshi ,  Anthia Toure ,  Steven Nagle ,  Ozge Whiting ,  George Harb ,  Matthew Sullivan ,  Maya Berlin-Udi ,  Stefanie Morgan ,  Nick Seay ,  Sang Lee ,  Scott Luro

IPC: G06T7/00 ,  B01L3/00 ,  B01L9/00 ,  C12M1/00 ,  C12M1/06 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  C12M1/36 ,  C12M3/00 ,  C12N5/00 ,  C12N5/074 ,  G01N15/10 ,  G01N15/14

CPC classification number: C12N5/0696 ,  B01L3/502715 ,  B01L3/502761 ,  B01L9/52 ,  C12M23/14 ,  C12M23/22 ,  C12M23/26 ,  C12M23/28 ,  C12M23/42 ,  C12M23/44 ,  C12M23/48 ,  C12M25/06 ,  C12M27/02 ,  C12M29/02 ,  C12M31/02 ,  C12M33/00 ,  C12M33/12 ,  C12M41/12 ,  C12M41/26 ,  C12M41/44 ,  C12M41/46 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0081 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1475 ,  G06T7/0012 ,  G06T7/0016 ,  B01L2200/18 ,  B01L2300/0663 ,  B01L2300/0877 ,  C12M41/36 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1454 ,  G01N2015/1486 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

公开(公告)号：US20240058817A1

公开(公告)日：2024-02-22

申请号：US18359810

申请日：2023-07-26

Applicant: The Regents of the University of Colorado, A Body Corporate

Inventor： Uday Kompella ,  Jonathan Taylor

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2400/0478 ,  B01L2300/023

Abstract: A system and method for nanoparticle synthesis employing an adhesiveless, deconstructable microfluidic mixing chip and an expandable wireless network of syringe pumps fluidly coupled to one or more microfluidic mixing chips. The wireless network of syringe pumps is controlled by a microprocessor with feedback from each of the syringe pumps in the network to allow for both individual, grouped and multiplexed control over the plurality of syringe pumps in the network.

公开(公告)号：US20240058813A1

公开(公告)日：2024-02-22

申请号：US17891636

申请日：2022-08-19

Applicant: Agilent Technologies, Inc.

Inventor： Jonathan Irribarren ,  Joel Myerson ,  Bo Curry

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655

Abstract: A fluid delivery device is configured to create a pressure differential across a compartment in which a liquid resides, causing the liquid to flow through the compartment. After a period of time, the fluid delivery device is configured to eliminate the pressure differential and thereby equilibrate the pressure across the compartment, with the use of a pressure equilibration channel that is separate from the compartment. The compartment may contain a packed bed of solid phase particles such as beads. In such case, the pressure differential causes the liquid to flow through the packed bed. The liquid may include chemical reagents or precursors that participate in chemical reactions on or at the solid phase particles. The reactions may relate to chemical synthesis, for example the synthesis of bio-chemicals such as nucleotides.

公开(公告)号：US11904314B2

公开(公告)日：2024-02-20

申请号：US17129783

申请日：2020-12-21

Applicant: WAINAMICS, INC.

Inventor： Arturo M. Escajeda ,  Huyen Tran ,  Ming X. Tan

IPC: C12Q1/6844 ,  B01L3/00

CPC classification number: B01L3/502715 ,  C12Q1/6844 ,  B01L2200/04 ,  B01L2200/0605 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/08

Abstract: The invention is directed to devices and methods for performing rapid low-cost bioassays in self-contained disposable cartridges that provide efficient mixing of sample and reactants under a layer of liquid wax. Some embodiments additionally use gravity assisted distribution of sample and assay reagents in conjunction with an appliance containing all necessary valves, pneumatic sources, heat sources and detection stations.

公开(公告)号：US11904312B2

公开(公告)日：2024-02-20

申请号：US16643264

申请日：2017-11-22

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Hilary Ely ,  Adam Higgins ,  Rachel M. White ,  Erik D. Torniainen ,  Tod Woodford ,  Michael W. Cumbie ,  Chien-Hua Chen

IPC: B01L3/00 ,  B81B1/00

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502723 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2400/0406 ,  B81B1/00

Abstract: The present disclosure is drawn to microfluidic devices. A microfluidic device can include a substrate, a lid mounted to the substrate, and a microchip mounted to the substrate. The lid mounted to the substrate can form a discrete microfluidic chamber between structures including an interior surface of the lid and a portion of the substrate. The lid can include an inlet and a vent positioned relative to one another to facilitate loading of fluid to the discrete microfluidic chamber via capillary action. A portion of the microchip can be positioned within the discrete microfluidic chamber.

公开(公告)号：US11904311B2

公开(公告)日：2024-02-20

申请号：US16596350

申请日：2019-10-08

Applicant: Remus Brix A. Haupt ,  John Harald Holm Arvidsen

Inventor： Remus Brix A. Haupt ,  John Harald Holm Arvidsen

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502707

Abstract: A microfluidic device with at least one integrated valve is provided for managing fluid flow in disposable assay devices, which provides constant flow even at very low flow rates. Pumps utilizing the microfluidic device, as well as methods for manufacture and performing a microfluidic process are also provided.

公开(公告)号：US11904310B2

公开(公告)日：2024-02-20

申请号：US15772035

申请日：2016-10-28

Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  DANA-FARBER CANCER INSTITUTE, INC.

Inventor： Anindita Basu ,  Christopher B. Ford ,  Aviv Regev ,  David A. Weitz ,  Asaf Rotem ,  Kevin Struhl

IPC: B01L3/00 ,  C12M3/06 ,  G01N33/50 ,  C12Q1/02 ,  C12M1/34 ,  C12Q1/18 ,  C12M1/32 ,  B01L3/02

CPC classification number: B01L3/502715 ,  B01L3/502746 ,  C12M23/12 ,  C12M23/16 ,  C12M41/46 ,  C12Q1/025 ,  C12Q1/18 ,  G01N33/502 ,  G01N33/5008 ,  G01N33/5011 ,  B01L3/0241 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/08 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/086

Abstract: The present invention generally relates to a controlled fluidic device to develop spatially complex environments to enhance the rate of evolution in cell populations. The method further provides an enhanced understanding in the emergence, for example, drug resistance during cancer chemotherapy.

公开(公告)号：US20240052433A1

公开(公告)日：2024-02-15

申请号：US18383270

申请日：2023-10-24

Applicant: Formulatrix International Holding Ltd.

Inventor： Kabir Yamana ,  Michael Nilsson ,  Sean Dineen ,  Rasmus Lindblom ,  Kyle Hallock

IPC: C12Q1/689 ,  C12Q1/6851 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6851 ,  B01L3/502715 ,  B01L7/52 ,  C12Q2600/106 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2200/04 ,  B01L2300/0883 ,  B01L2300/18

Abstract: In some aspects, a system is disclosed for detecting genetic targets for diagnosing tuberculosis and associated antibiotic resistance. The system includes a mechanical instrument and a cartridge assembly. The cartridge assembly comprises one or more reservoirs, with at least one reservoir containing a wet reagent. The cartridge also includes a chip with a fluidic channel holding a liquid slug. The chip has an optical detection region positioned between a heating region and a cool region. The mechanical instrument controls the back and forth motion of the liquid slug between the heating and cool regions. Additionally, the cartridge assembly includes one or more puncture elements configured to pierce the reservoirs and provide the wet reagent to the chip. The system further includes an optical detection unit with an optical light-emitting element, an optical detector, and a processing unit for analyzing the optical detection region of the chip.

公开(公告)号：US20240050948A1

公开(公告)日：2024-02-15

申请号：US17987978

申请日：2022-11-16

Applicant: CYTOAURORA BIOTECHNOLOGIES, INC.

Inventor： Chung-Er Huang ,  Sheng-Wen Chen ,  Hsin-Cheng Ho

IPC: B01L3/00 ,  B03C5/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B03C5/005 ,  B01L2300/0645 ,  B01L2400/0424 ,  B01L2200/0652

Abstract: A contactless selection device, a light sensing structure thereof, and a biological particle selection apparatus are provided. The light sensing structure includes a substrate, an insulating layer, an electrode layer, and a photoelectric layer, the latter two of which are respectively formed on two opposite sides of the substrate. The photoelectric layer includes a plurality of collector regions, a plurality of base regions respectively formed in the collector regions, and a plurality of emitter regions that are respectively formed in the base regions. Each of the emitter regions includes a plurality of emitter pads formed in the corresponding base region. Each of the base regions, the corresponding collector region, and the corresponding emitter region are jointly formed as a vertical transistor. The insulating layer covers and separates the vertical transistors and an end of each of the emitter pads is exposed from the insulating layer.

公开(公告)号：US20240044773A1

公开(公告)日：2024-02-08

申请号：US18491962

申请日：2023-10-23

Applicant: FUJIFILM Corporation

Inventor： Shota KAMEI ,  Takashi MORIMOTO ,  Tomoyuki SHIMODA

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1434 ,  B01L3/502715 ,  G01N15/1429 ,  B01L2300/0654 ,  B01L2300/027 ,  G01N2015/0065

Abstract: Provided are a cell life-and-death determination method, a cell life-and-death determination device, and a cell life-and-death determination system. The cell life-and-death determination method includes: acquiring images of a cell captured in a plurality of focal planes including an in-focus plane of the cell in a direction opposite to a side on which the cell is irradiated with light; acquiring an image piece including a central portion and an outer peripheral portion of the cell from each of the images; connecting the image pieces in an order of an imaging direction of the focal plane to create a connected image for analysis; extracting a feature amount from the connected image for analysis; and determining whether the cell is alive or dead on the basis of the feature amount of the connected image for analysis and a predetermined range of the feature amount.