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    MICROFLUIDIC LASER-ACTIVATED INTRACELLULAR DELIVERY SYSTEMS AND METHODS
    2.
    发明申请
    MICROFLUIDIC LASER-ACTIVATED INTRACELLULAR DELIVERY SYSTEMS AND METHODS 审中-公开

    公开(公告)号：US20190071695A1

    公开(公告)日：2019-03-07

    申请号：US16115140

    申请日：2018-08-28

    Applicant: CELLINO BIOTECH, INC.

    Inventor： Matthias Wagner ,  Marinna Madrid

    IPC: C12N15/87 ,  A61K9/00 ,  A61B18/20 ,  C12M1/42

    Abstract: An intracellular delivery system and method are provided. The intracellular delivery system comprises a laser-activated surface and cells positioned at a distance from the laser-activated surface. A laser provided a laser pulse that is used to porate membranes of the cells to deliver or extract cargo from the cells into a liquid surrounding the cells. The method of intracellular delivery comprises positioning a laser-activated surface at a distance from cells and applying a laser pulse from the laser to the surface to porate membranes of the cells to deliver or extract cargo from the cells into a liquid surrounding the cells.

    SYSTEM FOR IMAGE-DRIVEN CELL MANUFACTURING
    7.
    发明申请
    SYSTEM FOR IMAGE-DRIVEN CELL MANUFACTURING 有权

    公开(公告)号：US20210301247A1

    公开(公告)日：2021-09-30

    申请号：US17267383

    申请日：2019-08-09

    Applicant: CELLINO BIOTECH, INC.

    Inventor： Marinna Madrid ,  Matthias Wagner ,  Suvi Aivio ,  Stan Wang ,  Edroaldo Lummertz Da Rocha

    IPC: C12M1/34 ,  G06K9/00 ,  C12M1/00 ,  C12N5/0793 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  G01N15/14

    Abstract: Systems and methods for image-driven cell manufacturing are provided. Systems comprise a substrate for cells, imaging system for imaging cells on the substrate, computing system that computes cell characteristics from images, and a pulsed laser scanning system. The substrate is suitable for high-resolution cell imaging and is coated with a layer that partially absorbs laser pulses for the purpose of converting energy into microbubble formation. The computing system communicatively coupled to the pulsed laser scanning system and directs laser pulses to the substrate under targeted cells. Laser pulses are converted into mechanical energy via microbubbles that, depending on laser energy and pulse pattern, destroy selected cells, remove selected cells, or temporarily porate selected cells for the purpose of introducing biological cargos.

    SYSTEMS AND METHODS FOR CELL MANUFACTURING
    10.
    发明公开
    SYSTEMS AND METHODS FOR CELL MANUFACTURING 审中-公开

    公开(公告)号：US20240067914A1

    公开(公告)日：2024-02-29

    申请号：US18234342

    申请日：2023-08-15

    Applicant: Cellino Biotech, Inc.

    Inventor： Matthias Wagner ,  Suvi Aivio ,  Catherine Pilsmaker ,  Mariangela Amenduni ,  Amaldo Pereira ,  Sangkyun Lee ,  Scott Luro ,  Stefanie Morgan ,  Matthew Sullivan ,  Maya Berlin-Udi ,  Deniz Aksel ,  Jason Natale

    IPC: C12M1/34 ,  C12M1/42

    CPC classification number: C12M41/46 ,  C12M35/02

    Abstract: Systems and methods are disclosed for processes related to cell culture and generation. A method of manufacturing gene-edited cells comprises seeding a plurality of gene-edited cells into a cell culture container; culturing, by a cell culture system, the plurality of gene-edited cells into a plurality of gene-edited cell colonies; clonalizing, by the cell culture system, the plurality of gene-edited cell colonies by iterative spatially-selective removal of one or more portions from the plurality of gene-edited cell colonies as the colonies proliferate; tracking, by the cell culture system, one or more characteristics of the plurality of gene-edited clonal cell colonies; maintaining, by the cell culture system, a cell density of the plurality of gene-edited clonal cell colonies based on the tracked characteristics; selecting, by the cell culture system, a first gene-edited clonal cell colony from the plurality of gene-edited clonal cell colonies; removing, by the cell culture system, the plurality of gene-edited clonal cell colonies from the cell culture container except for the first gene-edited clonal cell colony; and expanding, by the cell culture system, the first gene-edited clonal cell colony.

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