公开(公告)号：US20240360488A1

公开(公告)日：2024-10-31

申请号：US18429780

申请日：2024-02-01

Applicant: 4BASEBIO, S.L.U. ,  4BASEBIO UK LTD

Inventor： Heikki Lanckriet ,  Amy Walker ,  Ángel Picher

IPC: C12P19/34 ,  C12N9/22 ,  C12N15/10

CPC classification number: C12P19/34 ,  C12N9/22 ,  C12N15/10 ,  C12N2310/122 ,  C12N2310/315 ,  C12Y207/07 ,  C12Y301/11001 ,  C12Y301/11002

Abstract: Methods for producing a linear deoxyribonucleic acid (DNA) product with enhanced resistance to nuclease digestion are provided. The methods comprise, (a) digesting a double-stranded DNA molecule with an endonuclease that cleaves an endonuclease target sequence to generate a digested double-stranded DNA molecule, wherein the digested double-stranded DNA molecule comprises a linear double-stranded region, and a truncated protelomerase sequence at a first end, wherein the truncated protelomerase target sequence is non-functional; (b) appending a first adaptor molecule to the first end of the digested double-stranded DNA molecule and appending a second adaptor molecule to the second end of the digested double-stranded DNA molecule to generate a precursor double-stranded DNA molecule, wherein the first adaptor molecule comprises a truncated protelomerase target sequence that forms a first functional protelomerase target sequence with the truncated protelomerase sequence at the first end of the digested double-stranded DNA molecule; and (c) incubating the precursor double-stranded DNA molecule with a protelomerase to generate the linear DNA product, wherein the protelomerase closes the first end of the precursor double-stranded DNA molecule at the first functional protelomerase target sequence.

公开(公告)号：US20240336904A1

公开(公告)日：2024-10-10

申请号：US18258021

申请日：2021-12-16

Applicant: 2seventy bio, Inc.

Inventor： JORDAN JARJOUR ,  ANNE-RACHEL KROSTAG

IPC: C12N9/22 ,  A61K35/17 ,  C12N5/0783 ,  C12N15/10

CPC classification number: C12N9/22 ,  A61K35/17 ,  C12N5/0636 ,  C12N15/102 ,  C07K2319/81 ,  C12N2510/00 ,  C12Y301/11001 ,  C12Y301/11002

Abstract: The present disclosure provides improved genome editing compositions and methods for editing a double-strand DNA target site. The disclosure further provides genome edited cells produced by the compositions and methods described.

公开(公告)号：US20180363042A1

公开(公告)日：2018-12-20

申请号：US16114424

申请日：2018-08-28

Applicant: ArcticZymes AS ,  Universitetet I Tromsø - Norges Arktiske Universitet

Inventor： Terese Solstad ,  Elisabeth Lill Andreassen ,  Marit Sjo Lorentzen ,  Olav Lanes ,  Morten Elde ,  Atle Noralf Larsen ,  Yvonne Piotrowski ,  Nils Peder Willassen

IPC: C12Q1/6848 ,  C12Q1/34 ,  G01N1/30 ,  C12N9/22

CPC classification number: C12Q1/6848 ,  C12N9/22 ,  C12Q1/34 ,  C12Q2521/325 ,  C12Y301/11001 ,  G01N1/30 ,  G01N2333/922

Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.

公开(公告)号：US20240076656A1

公开(公告)日：2024-03-07

申请号：US18215296

申请日：2023-06-28

Applicant: 10x Genomics, Inc.

Inventor： David Michael PATTERSON ,  Steven William SHORT ,  Dieter WILK ,  Pingwei ZHUANG ,  Solongo Batjargal ZIRALDO

IPC: C12N15/10 ,  B01J19/00 ,  B41M5/24 ,  C12N9/12 ,  C12N9/22 ,  G03F7/11

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B41M5/24 ,  C12N9/1264 ,  C12N9/22 ,  C12Y207/07031 ,  C12Y301/11001 ,  G03F7/11 ,  B01J2219/00855 ,  B01J2219/00927 ,  B01J2219/00993 ,  B01J2219/0894 ,  B01J2540/68

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate comprising a step of blocking or ablating oligonucleotide molecules in a boundary region separating a plurality of spot regions, and attaching oligonucleotides to oligonucleotide molecules in a first one or more of the spot regions.

公开(公告)号：US20170233800A1

公开(公告)日：2017-08-17

申请号：US15504507

申请日：2015-08-19

Applicant: ArcticZymes AS ,  Universitetet I Tromsø - Norges Arktiske Universitet

Inventor： Terese SOLSTAD ,  Elisabeth Lill ANDREASSEN ,  Marit Sjo LORENTZEN ,  Olav LANES ,  Morten ELDE ,  Atle Noralf LARSEN ,  Yvonne PIOTROWSKI ,  Nils Peder WILLASSEN

IPC: C12Q1/68 ,  G01N1/30

CPC classification number: C12Q1/6848 ,  C12N9/22 ,  C12Q1/34 ,  C12Q2521/325 ,  C12Y301/11001 ,  G01N1/30 ,  G01N2333/922

Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.

公开(公告)号：US20160230156A1

公开(公告)日：2016-08-11

申请号：US14776856

申请日：2014-03-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen HENDRICKS

IPC: C12N9/22 ,  C12N9/16

CPC classification number: C12N9/22 ,  C07K2319/00 ,  C12N9/16 ,  C12Y301/03001 ,  C12Y301/11001

Abstract: Disclosed herein is a fusion protein possessing both nuclease and phosphatase activities. The described fusion protein simplifies the processing of amplified DNA to degrade residual primers and nucleotide triphosphates and thereby facilitates subsequent DNA analysis.

Abstract translation: 本文公开了具有核酸酶和磷酸酶活性的融合蛋白。 所述的融合蛋白简化了扩增DNA的加工以降解残留引物和核苷酸三磷酸，从而有助于随后的DNA分析。