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公开(公告)号：US11560582B2
公开(公告)日：2023-01-24
申请号：US17251899
申请日：2019-06-19
Inventor： Peik Haugen , Miriam Grgic , Jenny Johansson Söderberg
Abstract: The present invention relates to fusion proteins comprising (i) a fusion moiety based on SEQ ID NO:1 and (ii) a protein. Also provided are nucleic acids encoding such fusion proteins and compositions comprising such fusion proteins. The invention also provides a method for increasing the expression level of a protein in a host cell or increasing the level of secretion of a protein from a host cell, said methods employing a fusion moiety in accordance with the invention. The invention further provides a method of producing a protein, said method comprising culturing an Aliivibrio wodanis host cell comprising a heterologous nucleic acid molecule encoding a protein under conditions suitable for the expression of the encoded protein. Certain deposited strains of Aliivibrio wodanis are also provided.
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公开(公告)号：US10787702B2
公开(公告)日：2020-09-29
申请号：US16539258
申请日：2019-08-13
Inventor： Terese Solstad , Elisabeth Lill Andreassen , Marit Sjo Lorentzen , Olav Lanes , Morten Elde , Atle Noralf Larsen , Yvonne Piotrowski , Nils Peder Willassen
IPC: C12Q1/6869 , C12Q1/6844 , C12N9/22 , C12Q1/6848 , C12Q1/34 , G01N1/30
Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.
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公开(公告)号：US20200071753A1
公开(公告)日：2020-03-05
申请号：US16539258
申请日：2019-08-13
Inventor： Terese SOLSTAD , Elisabeth Lill ANDREASSEN , Marit Sjo LORENTZEN , Olav LANES , Morten ELDE , Atle Noralf LARSEN , Yvonne PIOTROWSKI , Nils Peder WILLASSEN
IPC: C12Q1/6848 , C12Q1/34 , C12N9/22 , G01N1/30
Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.
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公开(公告)号：US20230092081A1
公开(公告)日：2023-03-23
申请号：US17823805
申请日：2022-08-31
Inventor： Marcin PIERECHOD , Nils Peder WILLASSEN , Ulli ROTHWEILER
IPC: C12Q1/6806 , C07K14/195
Abstract: The present invention relates to the use of a single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions, to dehybridize a DNA molecule or to prevent hybridisation of a complementary ssDNA, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the DNA molecule or ssDNA is present in or exposed to a solution containing one or more of the following (i) at least 350 mM of sodium ions; (ii) at least 50 mM of potassium ions; (iii) at least 150 mM of magnesium ions; or (iv) at least 200 mM of calcium ions.
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公开(公告)号：US11450800B2
公开(公告)日：2022-09-20
申请号：US16805301
申请日：2020-02-28
Inventor： Frank Melandsø , Sanat Wagle , Anowarul Habib
IPC: H01L41/083 , B32B27/06 , C08L27/12 , H01L41/053 , H01L41/187 , H01L41/193 , H01L41/29 , H04R17/00 , H01L41/23 , H01L41/45 , H01L41/37 , H01L41/00 , H01L41/08 , H01L41/16 , H01L41/27 , B32B15/04
Abstract: A film comprising a piezoelectric polymer has an upper surface and a lower surface. The film has an active region comprising the piezoelectric polymer, which extends from the upper surface of the film to the lower surface of the film. The film also comprises an adhesive sheet, which defines part of the upper or lower surface of the film. Circuit sheets may be bonded to the upper and lower surfaces in a lamination process to produce a laminated piezoelectric device.
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Patent Agency Ranking
公开(公告)号：US20210109334A1
公开(公告)日：2021-04-15
申请号：US16603105
申请日：2018-04-05
Inventor： Balpreet Singh AHLUWALIA , Thomas R. HUSER , Olav Gaute HELLESØ
Abstract: An optical component for illuminating a sample region with a periodic light pattern comprises: a first waveguide, a further waveguide and an optical splitter. The optical splitter has an input for receiving light, a first output and a second output. The first waveguide is optically coupled to the first output, to direct the first input light into the sample region in a first direction. The second output is optically coupled to the sample region to direct second input light into the sample region in a second direction. The further waveguide is arranged to receive third input light which is directed into the sample region in a third direction. The first direction, second direction and third direction are different from one another. The first and second input light interferes to form a periodic pattern in the sample region. The optical component may be used for structured illumination microscopy.
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公开(公告)号：US10787653B2
公开(公告)日：2020-09-29
申请号：US16087242
申请日：2017-03-22
Inventor： Atle Noralf Larsen , Yvonne Piotrowski , Netsanet Gizaw Assefa , Olav Lanes
IPC: C12N9/12 , C12Q1/6844
Abstract: The present invention relates to DNA polymerases. In particular, the present invention relates to DNA polymerases based on a DNA polymerase from a Psychrobacillus sp. The present invention provides an isolated DNA polymerase or an enzymatically active fragment thereof, said DNA polymerase comprising the amino acid sequence of SEQ ID NO:1 or comprising an amino acid sequence which is at least 70% identical to SEQ ID NO:1. The invention also provides nucleic acid molecules comprising a nucleotide sequence that encodes the DNA polymerase. The invention also provides a method of nucleotide polymerisation and a method of amplifying a nucleic acid in which the DNA polymerase or an enzymatically active fragment thereof is used.
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公开(公告)号：US20200024240A1
公开(公告)日：2020-01-23
申请号：US16495452
申请日：2018-03-28
Inventor： Morten Bøhmer Strøm , Annette Bayer , Stig Olov Magnus Engqvist , Marianne Hagensen Paulsen , Dominik Ausbacher
IPC: C07D239/62 , A61P31/04
Abstract: The present invention relates to bioactive cyclic compounds and their use as antimicrobial agents. In particular, the present invention relates to barbiturate mimics of Eusynstyelamides or small antimicrobial peptides. The compounds of the invention are represented by Formula (I). (I)
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公开(公告)号：US20170233800A1
公开(公告)日：2017-08-17
申请号：US15504507
申请日：2015-08-19
Inventor： Terese SOLSTAD , Elisabeth Lill ANDREASSEN , Marit Sjo LORENTZEN , Olav LANES , Morten ELDE , Atle Noralf LARSEN , Yvonne PIOTROWSKI , Nils Peder WILLASSEN
CPC classification number: C12Q1/6848 , C12N9/22 , C12Q1/34 , C12Q2521/325 , C12Y301/11001 , G01N1/30 , G01N2333/922
Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.
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公开(公告)号：US11454791B2
公开(公告)日：2022-09-27
申请号：US16603105
申请日：2018-04-05
Inventor： Balpreet Singh Ahluwalia , Thomas R. Huser , Olav Gaute Hellesø
Abstract: An optical component for illuminating a sample region with a periodic light pattern comprises: a first waveguide, a further waveguide and an optical splitter. The optical splitter has an input for receiving light, a first output and a second output. The first waveguide is optically coupled to the first output, to direct the first input light into the sample region in a first direction. The second output is optically coupled to the sample region to direct second input light into the sample region in a second direction. The further waveguide is arranged to receive third input light which is directed into the sample region in a third direction. The first direction, second direction and third direction are different from one another. The first and second input light interferes to form a periodic pattern in the sample region. The optical component may be used for structured illumination microscopy.
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