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    Methods and apparatus for measuring analytes using large scale FET arrays
    8.
    发明授权
    Methods and apparatus for measuring analytes using large scale FET arrays 有权

    公开(公告)号：US12140560B2

    公开(公告)日：2024-11-12

    申请号：US17820202

    申请日：2022-08-16

    Applicant: Life Technologies Corporation

    Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

    IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/423 ,  H01L29/78

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    Nucleic acid amplification
    9.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US11001815B2

    公开(公告)日：2021-05-11

    申请号：US16442341

    申请日：2019-06-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    Nucleic acid amplification
    10.
    发明授权
    Nucleic acid amplification 有权
    核酸扩增

    公开(公告)号：US09371557B2

    公开(公告)日：2016-06-21

    申请号：US13923232

    申请日：2013-06-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本教导提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。 聚合物可以包括筛分剂和/或扩散还原剂。

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