公开(公告)号：US09714915B2

公开(公告)日：2017-07-25

申请号：US14724354

申请日：2015-05-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

IPC: C12N9/12 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

公开(公告)号：US10059987B2

公开(公告)日：2018-08-28

申请号：US15625800

申请日：2017-06-16

Applicant: Life Technologies Corporation

Inventor： John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

IPC: C12N9/12 ,  C12Q1/6869 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.