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    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS
    1.
    发明申请
    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 审中-公开

    公开(公告)号：US20200024659A1

    公开(公告)日：2020-01-23

    申请号：US16535565

    申请日：2019-08-08

    Applicant: Life Technologies Corporation

    Inventor： Jonathan Schultz ,  John Davidson

    IPC: C12Q1/6869 ,  C12Q1/6874

    Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

    Linking methods, compositions, systems, kits and apparatuses
    3.
    发明授权
    Linking methods, compositions, systems, kits and apparatuses 有权

    公开(公告)号：US10385329B2

    公开(公告)日：2019-08-20

    申请号：US15786223

    申请日：2017-10-17

    Applicant: Life Technologies Corporation

    Inventor： John Davidson ,  Theo Nikiforov ,  Guobin Luo

    IPC: C12N9/12 ,  C12Q1/68 ,  C12N11/10

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

    Alternative nucleotide flows in sequencing-by-synthesis methods
    6.
    发明授权
    Alternative nucleotide flows in sequencing-by-synthesis methods 有权

    公开(公告)号：US10392660B2

    公开(公告)日：2019-08-27

    申请号：US15404735

    申请日：2017-01-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  John Davidson

    IPC: B01L3/00 ,  C12Q1/6869 ,  C12Q1/6874

    Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

    Alternative nucleotide flows in sequencing-by-synthesis methods
    9.
    发明授权
    Alternative nucleotide flows in sequencing-by-synthesis methods 有权
    按顺序排序的方法中的替代核苷酸流

    公开(公告)号：US09416413B2

    公开(公告)日：2016-08-16

    申请号：US13859673

    申请日：2013-04-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  John Davidson

    IPC: C12Q1/68 ,  B01L3/00

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

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