公开(公告)号：US20240355423A1

公开(公告)日：2024-10-24

申请号：US18684138

申请日：2021-08-25

申请人： Hewlett-Packard Development Company, L.P.

发明人： Yiming Zuo ,  Anton Wiranata ,  Amy Devitt ,  Yang Lei ,  Steven James Barcelo ,  Brian John Keefe

IPC分类号： G16B40/10 ,  C12Q1/6844 ,  G01N21/64 ,  G16B40/20

CPC分类号： G16B40/10 ,  C12Q1/6844 ,  G01N21/6408 ,  G16B40/20 ,  G01N2201/1296

摘要： Examples of methods are described herein. In some examples, a method includes determining signal variation data of a fluorescence signal measured from an amplification procedure of a nucleic acid sample. In some examples, the method includes detecting, using a machine learning model, a target nucleic acid strand in the nucleic acid sample based on the signal variation data.

公开(公告)号：US20240352545A1

公开(公告)日：2024-10-24

申请号：US18749700

申请日：2024-06-21

申请人： Gen-Probe Incorporated

发明人： Ankur Shah ,  Meghna Yadav

IPC分类号： C12Q1/70 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

CPC分类号： C12Q1/701 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

摘要： Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.

公开(公告)号：US20240336985A1

公开(公告)日：2024-10-10

申请号：US18749697

申请日：2024-06-21

申请人： Gen-Prob Incorporated

发明人： Ankur Shah ,  Meghna Yadav

IPC分类号： C12Q1/70 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

CPC分类号： C12Q1/701 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

摘要： Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.

公开(公告)号：US20240336970A1

公开(公告)日：2024-10-10

申请号：US18751153

申请日：2024-06-21

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  Matthew Micah Hill ,  Bernhard Zimmermann ,  Johan Baner ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Styrmir Sigurjonsson ,  Zachary Demko

IPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2600/156

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20240309456A1

公开(公告)日：2024-09-19

申请号：US18678417

申请日：2024-05-30

申请人： Natera, Inc.

发明人： MATTHEW RABINOWITZ ,  Matthew Micah HILL ,  Bernhard A. ZIMMERMANN ,  Johan BANER ,  George GEMELOS ,  Milena BANJEVIC ,  Allison RYAN ,  Styrmir SIGURJONSSON ,  Zachary DEMKO

IPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2600/156

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20240309436A1

公开(公告)日：2024-09-19

申请号：US18677287

申请日：2024-05-29

申请人： DnaNudge Limited

发明人： Christofer Toumazou ,  Rashmita Sahoo

IPC分类号： C12Q1/6844 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/6844 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/0803 ,  B01L2300/1805 ,  B01L2400/0487

摘要： A method of analysing a sample containing biological cells to detect the presence of a plurality of biomolecules, or a plurality of sequences within a biomolecule or biomolecules. The sample is lysed and introduced in the chamber of a cartridge comprising multiple chambers and an analysis unit. The sample is displaced to the analysis unit where isothermal or thermo-cycled pre-amplification is performed on the sample. Pre-amplification is followed by isothermal or thermo-cycled amplification to identify the presence of said plurality of biomolecules or sequences, or of a plurality of second biomolecules or sequences derived from the first mentioned plurality of biomolecules or sequences in a short time frame.

公开(公告)号：US20240309430A1

公开(公告)日：2024-09-19

申请号：US18608081

申请日：2024-03-18

申请人： The Hong Kong University of Science and Technology

发明人： I-Ming HSING ,  Xinyu ZHUANG

IPC分类号： C12Q1/6818 ,  C12Q1/6844

CPC分类号： C12Q1/6818 ,  C12Q1/6844

摘要： The subject invention pertains to the multiplex detection of nucleic acid molecules. This invention provides oligonucleotides labeled with a fluorophore and a quencher and oligonucleotides with target binding regions that are broadly compatible with the detection of multiple variant locus or multiple nucleic acid samples. Additionally, this disclosure also describes methods to label detection targets by asymmetric labeled amplification and differentiate the target sequences.

公开(公告)号：US20240301468A1

公开(公告)日：2024-09-12

申请号：US18652896

申请日：2024-05-02

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  Je-hyuk Lee ,  Richard C. Terry ,  Evan R. Daugharthy

IPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

摘要： Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US20240294983A1

公开(公告)日：2024-09-05

申请号：US18592402

申请日：2024-02-29

申请人： California Institute of Technology

发明人： Samuel J Schulte ,  Niles A Pierce

IPC分类号： C12Q1/6876 ,  C12Q1/6844

CPC分类号： C12Q1/6876 ,  C12Q1/6844 ,  C12Q2600/166

摘要： The present application relates to hybridization chain reaction (HCR). In particular, the sensitivity of hybridization chain reaction (HCR) signal amplification is combined with two or more fractional-initiator probes and one or more proximity probes able to colocalize a full HCR initiator that will trigger HCR when the targets are in proximity to one another.

公开(公告)号：US20240278249A1

公开(公告)日：2024-08-22

申请号：US18449422

申请日：2023-08-14

申请人： William Marsh Rice University

发明人： Dmitriy KHODAKOV ,  David ZHANG

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/6844

CPC分类号： B01L7/52 ,  B01L3/5027 ,  C12Q1/6844 ,  B01L2200/0663 ,  B01L2300/0819 ,  B01L2300/1805 ,  C12Q2565/513 ,  C12Q2565/629

摘要： The present disclosure provides methods, composition and devices for performing convection-based PCR and non-enzymatic amplification of nucleic acid sequences. Techniques and reagents employed in these methods include toehold probes, strand displacement reactions, Rayleigh-Benard convection, temperature gradients, multiplexed amplification, multiplexed detection, and DNA functionalization, in open and closed systems, for use in nucleic tests and assays.