A method of analysing a sample containing biological cells to detect the presence of a plurality of biomolecules, or a plurality of sequences within a biomolecule or biomolecules. The sample is lysed and introduced in the chamber of a cartridge comprising multiple chambers and an analysis unit. The sample is displaced to the analysis unit where isothermal or thermo-cycled pre-amplification is performed on the sample. Pre-amplification is followed by isothermal or thermo-cycled amplification to identify the presence of said plurality of biomolecules or sequences, or of a plurality of second biomolecules or sequences derived from the first mentioned plurality of biomolecules or sequences in a short time frame.