公开(公告)号：US12098407B2

公开(公告)日：2024-09-24

申请号：US17616843

申请日：2020-06-05

申请人： AMYRIS, INC.

发明人： Penelope R. Chua ,  Joshua Adam Lerman ,  Thomas Jon Scherbart ,  Chandresh Thakker ,  Annie Ening Tsong ,  Hanxiao Jiang

IPC分类号： C12N1/14 ,  C12N1/18 ,  C12N9/02 ,  C12N9/14 ,  C12N15/81 ,  C12P5/00

CPC分类号： C12P5/007 ,  C12N1/18 ,  C12N9/0036 ,  C12N9/14 ,  C12N15/81

摘要： Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.

公开(公告)号：US20240287560A1

公开(公告)日：2024-08-29

申请号：US17757752

申请日：2020-12-22

申请人： FIRMENICH SA

发明人： Laurent DAVIET ,  Yang HUANG ,  Vincent HARRACA ,  Michel SCHALK ,  Monica BANDERA

IPC分类号： C12P15/00 ,  A01N65/08 ,  A01P7/04 ,  A61K31/045 ,  A61K31/11 ,  A61K36/185 ,  C11B9/00 ,  C11B9/02 ,  C12N9/02 ,  C12N9/88 ,  C12P5/00 ,  C12P7/02

CPC分类号： C12P15/00 ,  A01N65/08 ,  A01P7/04 ,  A61K31/045 ,  A61K31/11 ,  A61K36/185 ,  C11B9/0003 ,  C11B9/02 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/88 ,  C12P5/007 ,  C12P7/02 ,  C12Y106/02004 ,  C12Y402/03082

摘要： Described herein is a biochemically produced sandalwood oil including at least 85% santalol and bergamotol and 1% or less cis-lanceol, where the oil has one or more of the following features: i) 5% or less alpha-santalal; ii) 5% or less farnesol; and iii) 0.5% or less spirosantalol. Also described herein are a perfuming composition including the sandalwood oil and a perfuming consumer product including the sandalwood oil. Also described herein are a method of using the biochemically produced sandalwood oil as an anti-microbial and anti-inflammatory agent, and an arthropod control composition including the sandalwood oil.

公开(公告)号：US20240150661A1

公开(公告)日：2024-05-09

申请号：US18248304

申请日：2021-10-06

申请人： Naoya YOSHIKAWA ,  Hiroyasu NAKAMURA

发明人： Naoya YOSHIKAWA ,  Hiroyasu NAKAMURA

IPC分类号： C10G32/00 ,  C10G31/08 ,  C10G53/02 ,  C12N9/20 ,  C12N9/50 ,  C12P5/00

CPC分类号： C10G32/00 ,  C10G31/08 ,  C10G53/02 ,  C12N9/20 ,  C12N9/63 ,  C12P5/00 ,  C10G2300/202 ,  C10G2300/302 ,  C10G2300/304 ,  C10G2300/307 ,  C10G2300/308 ,  C10G2400/04

摘要： [Issues] To provide a method to produce diesel fuel that meets the standards of almost all countries in a short time.
[Solution] A method for producing diesel fuel having improved properties by mixing an aqueous enzyme solution containing 0.01 to 0.1% by weight of a lipase enzyme as a first enzyme with a water-soluble alcohol so as to be an aqueous alcohol solution having an alcohol concentration of from 20 to 35%, comprises adding a second enzyme solution in light oil having 0.001 to 0.01% by weight of pineapple-derived enzyme containing bromelain, dissolved in light oil to said base oil.

公开(公告)号：US20240141392A1

公开(公告)日：2024-05-02

申请号：US18373659

申请日：2023-09-27

申请人： Givaudan SA ,  MANUS BIO INC.

发明人： Ajikumar Parayil Kumaran ,  Chin Giaw Lim ,  Liwei Li ,  Souvik Ghosh ,  Christopher Pirie ,  Anthony Qualley ,  Geoff Marshall-Hill ,  Martin Preininger

IPC分类号： C12P7/26 ,  A01N27/00 ,  A23L27/12 ,  C12N9/02 ,  C12P5/00

CPC分类号： C12P7/26 ,  A01N27/00 ,  A23L27/13 ,  C12N9/0073 ,  C12P5/002 ,  C12Y114/13078 ,  A23V2002/00 ,  C07K2319/03

摘要： The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.

公开(公告)号：US20240084343A1

公开(公告)日：2024-03-14

申请号：US18464101

申请日：2023-09-08

申请人： Kiverdi, Inc.

发明人： John S. Reed ,  Itzhak Kurek ,  Henrik Fyrst ,  Lisa Dyson

IPC分类号： C12P13/22 ,  C12P5/00 ,  C12P7/18 ,  C12P7/625 ,  C12P7/6409 ,  C12P13/00 ,  C12P13/08 ,  C12P17/10

CPC分类号： C12P13/225 ,  C12P5/005 ,  C12P7/18 ,  C12P7/625 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/08 ,  C12P13/222 ,  C12P17/10

摘要： Aspects of the invention relate to methods of producing small molecules for industrial application using natural organisms and engineered organisms.

公开(公告)号：US20240060075A1

公开(公告)日：2024-02-22

申请号：US18454635

申请日：2023-08-23

申请人： AMYRIS, INC.

发明人： Andrew MAIN ,  Grzegorz WOJCIECHOWSKI ,  Yue YANG ,  Lishan ZHAO

IPC分类号： C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/00

CPC分类号： C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/007 ,  C12Y205/01029 ,  C12Y402/03015

摘要： Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US11873220B2

公开(公告)日：2024-01-16

申请号：US17222217

申请日：2021-04-05

申请人： Iogen Corporation

发明人： Brian Foody

IPC分类号： C01B3/34 ,  C10G65/02 ,  C10G45/02 ,  C10G47/00 ,  C10G65/12 ,  C12P5/02 ,  C10L3/08 ,  C10L3/10 ,  C07C5/02 ,  C12P5/00 ,  G06Q30/018 ,  C01B3/36 ,  C10G45/44 ,  C10L1/04 ,  G06Q50/06

CPC分类号： C01B3/34 ,  C01B3/36 ,  C07C5/02 ,  C10G45/02 ,  C10G45/44 ,  C10G47/00 ,  C10G65/02 ,  C10G65/12 ,  C10L1/04 ,  C10L3/08 ,  C10L3/10 ,  C10L3/101 ,  C12P5/00 ,  C12P5/02 ,  C12P5/023 ,  G06Q30/018 ,  G06Q50/06 ,  C01B2203/0211 ,  C01B2203/0216 ,  C01B2203/0222 ,  C01B2203/065 ,  C01B2203/1241 ,  C10G2400/02 ,  C10G2400/04 ,  C10G2400/08 ,  C10L2200/0469 ,  C10L2290/26 ,  Y02E50/10 ,  Y02E50/30 ,  Y02P20/133 ,  Y02P30/00 ,  Y02P30/20 ,  Y02P80/20

摘要： According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the liquid hydrocarbon with the renewable hydrogen or alternatively, the renewable hydrogen can be added to a reactor operated so as to simultaneously desulfurize and hydrogenate the hydrocarbons. The present invention enables a party to receive a renewable fuel credit for the transportation or heating fuel.

公开(公告)号：US11845940B2

公开(公告)日：2023-12-19

申请号：US17187697

申请日：2021-02-26

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  NATIONAL TECHNOLOGY AND ENGINEERING SOLUTIONS OF SANDIA, LLC

发明人： Gina Marie Geiselman ,  James Kirby ,  Taek Soon Lee ,  John M. Gladden ,  Blake A. Simmons

IPC分类号： C12N15/81 ,  C12N9/88 ,  C12P5/00 ,  C12N1/16 ,  C10L1/04

CPC分类号： C12N15/815 ,  C10L1/04 ,  C12N1/16 ,  C12N9/88 ,  C12P5/007 ,  C12Y402/03037 ,  C10L2200/043 ,  C10L2270/04 ,  C10L2290/26

摘要： The present invention provides for a genetically modified fungal host cell capable of producing prespatane and/or epi-isozizaene comprising prespatane synthase (PPS) and/or epi-isozizaene synthase (EIZS).

公开(公告)号：US20230323314A1

公开(公告)日：2023-10-12

申请号：US18161938

申请日：2023-01-31

申请人： MANUS BIO INC.

发明人： Jason DONALD ,  Christopher PIRIE ,  Liwei LI ,  Huey-Ming MAK ,  Srishti TIBREWALA ,  Ajikumar PARAYIL KUMARAN

IPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/02 ,  C12P19/56 ,  C12P5/00 ,  C12N15/70

CPC分类号： C12N9/0079 ,  C12N9/00 ,  C12N15/52 ,  C12N15/70 ,  C12P5/007 ,  C12P19/56 ,  C07K2319/03

摘要： The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.

公开(公告)号：US20230167457A1

公开(公告)日：2023-06-01

申请号：US17787493

申请日：2020-12-20

申请人： ENGENES BIOTECH GMBH

发明人： Juergen Mairhofer

IPC分类号： C12N15/70 ,  C12M1/06 ,  C12M1/00 ,  C12P5/00

CPC分类号： C12N15/70 ,  C12M27/02 ,  C12M23/58 ,  C12P5/007

摘要： The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression and nucleotide production. The invention generally relates to systems and processes that are suitable or comprise a two stage production process, in which the growth of the bacterial host cell is spatiotemporally separated from the production of the protein or nucleic acid of interest. Accordingly, the present invention relates to a system and a process for use in continuous production of a protein of interest or a nucleotide of interest by a bacterial host cell.