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    Method of large scale growth of toxoplasmic microorganisms
    1.
    发明授权
    Method of large scale growth of toxoplasmic microorganisms 失效
    大量生长弓形虫微生物的方法

    公开(公告)号：US4824666A

    公开(公告)日：1989-04-25

    申请号：US120041

    申请日：1987-11-13

    申请人： D. Craig Wright

    发明人： D. Craig Wright

    IPC分类号： A61K39/002 ,  C12N1/10 ,  G01N33/569 ,  G01N33/54

    CPC分类号： A61K39/002 ,  C12N1/10 ,  G01N33/56905

    摘要： A method of producing large scale growth of parasites of the genus Toxoplasma is disclosed which comprises inoculating the microorganisms into a non-adherent human cell line, such as a monocytoid lymphoma cell line, and maintaining the cell line in tissue culture media capable of supporting the growth of the microorgranism while the cell line is being maintained. This large scale production allows for the development of vaccines for Toxoplasma microorganisms which previously have been hard to grow in the numbers needed to readily prepare vaccines, and allows for more rapid antibody detection procedures with regard to these microorganisms. There are also disclosed methods for preparing such vaccines, and methods for carrying out antibody detection assays.

    摘要翻译： 公开了一种产生弓形虫属寄生虫大规模生长的方法，其包括将微生物接种到非贴壁人细胞系如单核细胞淋巴瘤细胞系中，并将细胞系保持在能够支持 在保持细胞系的同时微生物的生长。 这种大规模生产允许开发以前难以在容易制备疫苗所需的数量生长的弓形虫微生物的疫苗，并且允许针对这些微生物的更快速的抗体检测程序。 还公开了制备这种疫苗的方法和用于进行抗体检测测定的方法。

    Apparatus for testing reactions
    4.
    发明授权
    Apparatus for testing reactions 失效
    用于测试反应的装置

    公开(公告)号：US4657868A

    公开(公告)日：1987-04-14

    申请号：US428172

    申请日：1982-09-29

    申请人： Rolf Saxholm

    发明人： Rolf Saxholm

    IPC分类号： C12M1/20 ,  C12M1/22 ,  C12M1/00 ,  C12M1/28 ,  C12Q1/22 ,  G01N33/54

    CPC分类号： C12M35/06 ,  C12M23/10 ,  C12M41/46 ,  G01N2800/52 ,  Y10S436/806

    摘要： A biologically active substance is incorporated in a unitary body with a magnetically responsive material for carrying out diffusion testing. These may be, microbiological, immunological, serological and other biochemical examinations. The body is applied against a substrate or medium by application of an external magnetic field and a reaction region is produced at the site of the body and is measured by means of a reader. In order to insure deposit of the body on the substrate a predetermined location and corresponding reading of the reaction region at such location, the support for the substrate and the dispenser and rear are provided with suitable releasable coupling and orienting devices such that the dispenser and reader can be respectively engaged and oriented on the support in predetermined secured positions.

    摘要翻译： 将生物活性物质并入具有用于进行扩散测试的磁响应材料的整体中。 这些可能是微生物，免疫学，血清学和其他生物化学检查。 通过施加外部磁场将身体施加到衬底或介质上，并且在身体部位产生反应区域，并通过读取器测量。 为了确保本体在基底上的沉积物在该位置处的预定位置和反应区域的对应读取，对基底和分配器和后部的支撑件设置有合适的可释放的联接和定向装置，使得分配器和读取器 可以分别在预定的固定位置上在支撑件上接合和取向。

    Monoclonal antibodies to skin cells
    6.
    发明授权
    Monoclonal antibodies to skin cells 失效
    皮肤细胞单克隆抗体

    公开(公告)号：US4649115A

    公开(公告)日：1987-03-10

    申请号：US480717

    申请日：1983-03-31

    申请人： Bijan Safai ,  Edward A. Boyse ,  Fung-Win Shen

    发明人： Bijan Safai ,  Edward A. Boyse ,  Fung-Win Shen

    IPC分类号： C07K16/28 ,  G01N33/54 ,  C12N5/00 ,  C12N15/00 ,  C12R1/91

    CPC分类号： C07K16/28 ,  Y10S435/948

    摘要： Successive layers of guinea pig epidermis display discrete antigenic markers that are recognized by a selected panel of five monoclonal antibodies produced by hybridomas resulting from immunization of mice with suspensions of dissociated viable guinea pig epidermal cells. Antigen to Gpsk-1 marks the basement membrane, which membrane is probably produced by basal cells. Antigen to Gpsk-2 is expressed by basal and suprabasal cells. Antigens to both Gpsk-3 and Gpsk-4 occur on the spinous and overlying layers but are distinguished by differences in their representation on certain non-epidermal cell types and on other epithelia. Antigen to Gpsk-5 is found on basement membrane and on spinous and overlying granular and horny cells. Antigens to Gpsk-2 through 5 are situated at the cell surface and may recognize integral plasma membrane molecules expressed in differentiative sequence. The five antibodies differ also in their antigenic marker distribution among epithelial as well as other selected tissue types. These findings provide a background to potential dermatological applications of monoclonal antibodies to human epidermis.

    摘要翻译： 豚鼠表皮的连续层显示离散的抗原标记，其由选择的由5只单克隆抗体产生的单克隆抗体识别，所述抗体由通过解离的活的豚鼠表皮细胞的悬浮液免疫小鼠而产生的杂交瘤产生。 Gpsk-1的抗原标记基底膜，该膜可能是由基底细胞产生的。 Gpsk-2的抗原由基底细胞和上基底细胞表达。 Gpsk-3和Gpsk-4的抗原发生在棘突和上覆层上，但是由于它们在某些非表皮细胞类型和其它上皮细胞上的表达差异， Gpsk-5的抗原在基底膜和棘突和上覆的颗粒状和角质细胞上发现。 Gpsk-2至5的抗原位于细胞表面，并且可以识别以差异序列表达的整合质膜分子。 五种抗体在上皮以及其他选择的组织类型中的抗原性标记分布方面也存在差异。 这些发现为单克隆抗体对人类表皮的潜在皮肤病学应用提供了背景。

    Latex agglutination determination of IgG in neonatals and in colostrum
    7.
    发明授权
    Latex agglutination determination of IgG in neonatals and in colostrum 失效
    新生儿和初乳中IgG的乳胶凝集测定

    公开(公告)号：US4542103A

    公开(公告)日：1985-09-17

    申请号：US552482

    申请日：1983-11-16

    申请人： Ernest C. Adams

    发明人： Ernest C. Adams

    IPC分类号： G01N33/543 ,  G01N33/54

    CPC分类号： G01N33/54313

    摘要： A method for determining the amount of IgG present in a neonatal foal or calf or in the colostrum of a dam or cow. The method involves contacting a body fluid sample with biologically inert latex particles, measuring the amount of agglutination which occurs, and determining the amount of IgG present. The latex particles are diluted to a final concentration of from about 0.25 to 2.0 percent (w/v) at a pH of from about 7.5 to 9.0 with a buffer. The body fluid is diluted to a range of from 0.01:5 parts to 0.01:2160 parts, v/v basis, at a pH of from about 7.5 to 9.0.

    摘要翻译： 用于确定存在于新生儿驹或小腿中或存在于大坝或母牛初乳中的IgG的量的方法。 该方法包括使体液样品与生物惰性胶乳颗粒接触，测量发生的凝集的量，并确定存在的IgG的量。 用缓冲液将胶乳颗粒在约7.5至9.0的pH下稀释至约0.25至2.0％（w / v）的终浓度。 将体液以约7.5至9.0的pH稀释至0.01:5份至0.01:2160份，v / v的范围。

    Soluble insoluble polymers in enzymeimmunoassay
    8.
    发明授权
    Soluble insoluble polymers in enzymeimmunoassay 失效
    可溶性不溶性聚合物在酶免疫测定中

    公开(公告)号：US4530900A

    公开(公告)日：1985-07-23

    申请号：US417281

    申请日：1982-09-13

    申请人： David L. Marshall

    发明人： David L. Marshall

    IPC分类号： G01N33/539 ,  G01N33/548 ,  G01N33/54 ,  C12N9/96

    CPC分类号： G01N33/539 ,  G01N33/548 ,  Y10S435/969 ,  Y10S435/971 ,  Y10S436/815 ,  Y10S436/824 ,  Y10S436/828

    摘要： The invention pertains to an improved heterogeneous enzymeimmunoassay involving the use of a reversibly soluble polymeric substance acting as the support for the antibody. In the direct method the antigen to be detected and an enzyme labeled antigen are bound by antibody which is chemically linked to the soluble polymeric substance. The polymer is rendered insoluble and removed from the test solution. After resolubilization into a solution containing substrate for the enzyme label, the assay for antigen is completed by determination of enzymatic activity. In the indirect method, the antigen to be detected and an enzyme labeled antigen are incubated with a primary antibody unattached to the polymeric substance. After addition of a second antibody which is chemically linked to the polymeric substance, the polymer is rendered insoluble and the assay is performed as in the direct method described above.

    摘要翻译： 本发明涉及一种改进的异源酶免疫测定法，其涉及使用作为抗体载体的可逆溶解聚合物质。 在直接法中，待检测的抗原和酶标记的抗原通过与可溶性聚合物质化学连接的抗体结合。 使聚合物变得不溶，并从测试溶液中除去。 在重新溶解成含有酶标记底物的溶液后，通过测定酶活性来完成抗原测定。 在间接方法中，将待检测的抗原和酶标记的抗原与未附着于聚合物质的一抗孵育。 加入与聚合物质化学连接的第二抗体后，使聚合物变得不溶，并按照上述直接方法进行测定。

    Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations
    10.
    发明授权
    Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations 失效
    用于过氧化物酶测定的催化比色和荧光底物

    公开(公告)号：US4521511A

    公开(公告)日：1985-06-04

    申请号：US421263

    申请日：1982-09-22

    申请人： Robert L. Stout

    发明人： Robert L. Stout

    IPC分类号： G01N21/76 ,  C12Q1/28 ,  G01N33/52 ,  G01N33/54

    CPC分类号： C12Q1/28 ,  C12Q2326/30 ,  Y10S435/805 ,  Y10S436/826 ,  Y10S436/904 ,  Y10T436/206664

    摘要： Improved catalyzed substrates for use in developing characteristic colors or fluorescence in the presence of peroxidase enzymes (e.g., horseradish peroxidase) are disclosed which include as a rate accelerator a substituted phenol such as a p-halogenated phenol. The complete system typically includes a peroxide type oxidizing agent (e.g., hydrogen peroxide), a chromogenic or flurogenic compound (e.g., ABTS), a buffer and the accelerator compound. Advantageously, the accelerator should provide at least about 50 percent rate enhancement for the substrate, as compared with an otherwise identical, accelerator-free substrate reacted under the same conditions; however, the most preferred accelerator, p-iodophenol, gives enhancements on the order to 1,000 percent. The invention is particularly useful in so-called ELISA determinations which involve an enzyme-linked moiety, and permit detection at very low concentration levels unobtainable with conventional colorimetric substrates.

    摘要翻译： 公开了用于在过氧化物酶（例如辣根过氧化物酶）存在下开发特征颜色或荧光的改进的催化底物，其包括作为速率促进剂的取代的苯酚，例如对卤代苯酚。 完整的系统通常包括过氧化物型氧化剂（例如过氧化氢），显色或致毛化合物（例如ABTS），缓冲剂和促进剂化合物。 有利的是，与在相同条件下反应的另外相同的无促进剂的底物相比，促进剂应为基材提供至少约50％的速率增强; 然而，最优选的加速剂对 - 碘苯酚使订单增加到1,000％。 本发明特别可用于涉及酶联系部分的所谓ELISA测定，并允许以非常低的浓度水平进行检测，而常规比色底物无法获得。