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    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof
    1.
    发明申请
    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其应用

    公开(公告)号：US20080010026A1

    公开(公告)日：2008-01-10

    申请号：US11714887

    申请日：2007-03-07

    Applicant: Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    Inventor： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    IPC: G06F19/00

    CPC classification number: C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在采食稀少糖的策略（图1）中，旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系，因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质（a）或（b）的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法，其特征在于，在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞，从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖，其特征在于，在单糖的总体图中，目标稀有糖的位置被理解，因此设计了允许上述蛋白质起作用的最佳生产途径。

    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof
    4.
    发明授权
    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其应用

    公开(公告)号：US07501267B2

    公开(公告)日：2009-03-10

    申请号：US11714887

    申请日：2007-03-07

    Applicant: Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    Inventor： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    IPC: C12P21/06 ,  C07H21/04 ,  C12N1/21 ,  C12N9/90

    CPC classification number: C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在采食稀少糖的策略（图1）中，旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系，因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质（a）或（b）的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法，其特征在于，在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞，从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖，其特征在于，在单糖的总体图中，目标稀有糖的位置被理解，因此设计了允许上述蛋白质起作用的最佳生产途径。

    Gene sequence of l-rhamnose isomerase having new catalytic function and use thereof
    5.
    发明申请
    Gene sequence of l-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其用途

    公开(公告)号：US20060148061A1

    公开(公告)日：2006-07-06

    申请号：US10541822

    申请日：2004-01-09

    Applicant: Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    Inventor： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    IPC: C07H21/04 ,  C12P21/06 ,  C12P19/02 ,  C12N9/90 ,  C12N15/74 ,  C12N1/21

    CPC classification number: C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在采食稀少糖的策略（图1）中，旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系，因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质（a）或（b）的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法，其特征在于，在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞，从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖，其特征在于，在单糖的总体图中，目标稀有糖的位置被理解，因此设计了允许上述蛋白质起作用的最佳生产途径。

    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof
    7.
    发明授权
    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其应用

    公开(公告)号：US07205141B2

    公开(公告)日：2007-04-17

    申请号：US10541822

    申请日：2004-01-09

    Applicant: Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    Inventor： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

    IPC: C07H21/04 ,  C12P21/06 ,  C12N9/90 ,  C12N1/21

    CPC classification number: C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在“稀释糖消化策略”（

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