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公开(公告)号:US20240002828A1
公开(公告)日:2024-01-04
申请号:US18039405
申请日:2021-11-30
Inventor: Kazuya AKIMITSU , Ken IZUMORI , Akihide YOSHIHARA , Shiro KATO , Susumu MOCHIZUKI , Hiromi YOSHIDA , Shigehiro KAMITORI
IPC: C12N9/90 , C07K14/265 , C12N15/74 , C12P19/02 , C12N11/08
CPC classification number: C12N9/90 , C07K14/265 , C12N15/74 , C12P19/02 , C12N11/08 , C12Y503/01014 , C12R2001/01
Abstract: A highly safe and highly heat-resistant L-rhamnose isomerase usable in the food industry and the production thereof are disclosed. The L-rhamnose isomerase is derived from a microorganism belonging to the genus Enterobacter, has a subunit molecular mass of 47 kDa as measured by SDS-PAGE, and has the following substrate specificities (A) isomerase activity that recognizes and reacts with a CHO group at C1 and an OH group at C2 of an aldose to convert the CHO group at C1 into an OH group and the OH group at C2 into a CO group, or recognizes and reacts with an OH group at C1 and a CO group at C2 of a ketose to convert the OH group at C1 into a CHO group and the CO group at C2 into an OH group; and (B) reactivity with L-rhamnose, L-lyxose, L-mannose, D-allose, D-ribose, and L-talose.