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公开(公告)号：US11421215B2
公开(公告)日：2022-08-23
申请号：US17044583
申请日：2018-03-15
Applicant: PETIVA PRIVATE LIMITED
Abstract: The present invention provides for a nucleic acid encoding an isomerase and uses of the isomerase for bioconversion of sugar substrates. The invention represents an advancement in the field of enzyme engineering and discloses a modified nucleic acid for achieving optimum expression of a protein having isomerase activity in a heterologous host. The invention also discloses vectors carrying the modified nucleic acid and recombinant host cells carrying the vectors. The invention also discloses the process for producing a recombinant host cell, process for production of the recombinant enzyme and the process for bioconversion of sugars into their respective isomers using the recombinant protein.
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Patent Agency Ranking
公开(公告)号：US09752170B2
公开(公告)日：2017-09-05
申请号：US14655034
申请日：2013-04-17
Applicant: Petiva Private Limited
Inventor： Saravanakumar Iyappan , Samir Kumar Roy , Saju Varghese , Aparna Devi Addala , Venkata Narayanan Karthikeyan , Kanumuru Rahul Raju , Banibrata Pandey
CPC classification number: C12P19/24 , C12N9/90 , C12P19/02 , C12Y501/03 , C12Y503/01014
Abstract: The present invention is directed towards genetic modification of native gene encoding for D-tagatose 3-epimerase and rhamnose isomerase to substantially increase the expression level of these enzymes and use of the enzymes in a process to produce rare monosaccharides such as psicose and allose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.
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公开(公告)号：US20170267989A1
公开(公告)日：2017-09-21
申请号：US15605816
申请日：2017-05-25
Applicant: Petiva Private Limited
Inventor： Samir Kumar Roy , Saravanakumar Iyappan , Saju Varghese , Aparna Devi Addala , Milky Agarwal , Kanumuru Rahul Raju , Banibrata Pandey
CPC classification number: C12N9/90 , C12P19/12 , C12P19/24 , C12Y504/99011
Abstract: The present invention is directed towards genetic modification of native gene encoding for sucrose isomerase and isomaltulose synthase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare disaccharides such as isomaltulose and trehalulose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.
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公开(公告)号：US09695411B2
公开(公告)日：2017-07-04
申请号：US14655035
申请日：2013-04-17
Applicant: Petiva Private Limited
Inventor： Samir Kumar Roy , Saravanakumar Iyappan , Saju Varghese , Aparna Devi Addala , Milky Agarwal , Kanumuru Rahul Raju , Banibrata Pandey
CPC classification number: C12N9/90 , C12P19/12 , C12P19/24 , C12Y504/99011
Abstract: The present invention is directed towards genetic modification of native gene encoding for sucrose isomerase and isomaltulose synthase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare disaccharides such as isomaltulose and trehalulose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.
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公开(公告)号：US20190000116A1
公开(公告)日：2019-01-03
申请号：US16061969
申请日：2016-11-12
Applicant: Petiva Private Limited
Inventor： Banibrata Pandey , Rahul Raju Kanumuru , Saravanakumar Iyappan , Karthikeyan Venkata Narayanan , Humaira Parveen Sheikh
Abstract: The present invention provides a process for preparing non-cariogenic, sustained energy release juice. The process comprises contacting juice with an enzyme immobilized on Duolite at 30-50° C. for 1-5 h; wherein the enzyme is capable of converting cariogenic sugar to non-cariogenic sugar; and separating juice from the enzyme complex.
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公开(公告)号：US09885030B2
公开(公告)日：2018-02-06
申请号：US15605816
申请日：2017-05-25
Applicant: Petiva Private Limited
Inventor： Samir Kumar Roy , Saravanakumar Iyappan , Saju Varghese , Aparna Devi Addala , Milky Agarwal , Kanumuru Rahul Raju , Banibrata Pandey
CPC classification number: C12N9/90 , C12P19/12 , C12P19/24 , C12Y504/99011
Abstract: The present invention is directed towards genetic modification of native gene encoding for sucrose isomerase and isomaltulose synthase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare disaccharides such as isomaltulose and trehalulose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.
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