公开(公告)号：US20150361473A1

公开(公告)日：2015-12-17

申请号：US14655034

申请日：2013-04-17

Applicant: AGTIVE BIO-SCIENCES PRIBATE LIMITED

Inventor： Saravanakumar Iyappan ,  Samir Kumar Roy ,  Saju Varghese ,  Aparna Devi Addala ,  Venkata Narayanan Karthikeyan ,  Kanumuru Rahul Raju ,  Banibrata Pandey

IPC: C12P19/24 ,  C12P19/02 ,  C12N9/90

CPC classification number: C12P19/24 ,  C12N9/90 ,  C12P19/02 ,  C12Y501/03 ,  C12Y503/01014

Abstract: The present invention is directed towards genetic modification of native gene encoding for D-tagatose 3-epimerase and rhamnose isomerase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare monosaccharides such as psicose and allose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.

Abstract translation: 本发明涉及编码D-塔格糖3-差向异构酶和鼠李糖异构酶的天然基因的遗传修饰，以显着增加这些酶的表达水平，并且在生产稀有单糖如精巧糖和阿洛糖的方法中使用所述酶。 本发明还公开了包含修饰基因的表达构建体和表达该基因的宿主细胞。