METHOD FOR REMOVING INTRA-MICROCHANNEL BUBBLES AND INTRA-MICROCHANNEL DISSOLVING AND DISPERSING METHOD
    1.
    发明申请
    METHOD FOR REMOVING INTRA-MICROCHANNEL BUBBLES AND INTRA-MICROCHANNEL DISSOLVING AND DISPERSING METHOD 审中-公开
    去除微通道泡沫和微通道溶解和分散方法的方法

    公开(公告)号:US20090126568A1

    公开(公告)日:2009-05-21

    申请号:US12271784

    申请日:2008-11-14

    Abstract: A method for removing intra-microchannel bubbles, which removes bubbles occurring in a microchannel, is provided; the method including: allowing a liquid that contains bubbles and is introduced into a microchannel to flow in a first direction at a first flow speed at which the bubbles float upward and can remain adhered on an inner wall of the microchannel or less; and then allowing the liquid to flow in a second direction that is opposite to the first direction to move a gas-liquid interface of the liquid, which is a rear end of the liquid in the second direction, in the second direction at a second flow speed at which the bubbles adhered on the inner wall of the microchannel can maintain an adhesion position so as to collect the bubbles on the gas-liquid interface and make the bubbles disappear by exposing the bubbles to a gas.

    Abstract translation: 提供了一种去除微通道气泡的方法,其去除了在微通道中发生的气泡; 所述方法包括:允许含有气泡并被引入微通道的液体以气泡向上浮动并保持粘附在微通道内壁上的第一流速沿第一方向流动; 然后允许液体沿与第一方向相反的第二方向流动,以第二方向在第二方向上移动作为第二方向的液体后端的液体的气液界面 粘附在微通道内壁上的气泡的速度可以保持粘合位置,以便在气 - 液界面上收集气泡,并通过将气泡暴露于气体而消失气泡。

    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof
    2.
    发明授权
    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其应用

    公开(公告)号:US07501267B2

    公开(公告)日:2009-03-10

    申请号:US11714887

    申请日:2007-03-07

    CPC classification number: C12N9/90 C12P19/02 C12P19/24 C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在采食稀少糖的策略(图1)中,旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系,因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质(a)或(b)的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO:2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法,其特征在于,在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞,从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖,其特征在于,在单糖的总体图中,目标稀有糖的位置被理解,因此设计了允许上述蛋白质起作用的最佳生产途径。

    Gene sequence of l-rhamnose isomerase having new catalytic function and use thereof
    3.
    发明申请
    Gene sequence of l-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其用途

    公开(公告)号:US20060148061A1

    公开(公告)日:2006-07-06

    申请号:US10541822

    申请日:2004-01-09

    CPC classification number: C12N9/90 C12P19/02 C12P19/24 C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在采食稀少糖的策略(图1)中,旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系,因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质(a)或(b)的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO:2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法,其特征在于,在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞,从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖,其特征在于,在单糖的总体图中,目标稀有糖的位置被理解,因此设计了允许上述蛋白质起作用的最佳生产途径。

    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof
    4.
    发明授权
    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其应用

    公开(公告)号:US07205141B2

    公开(公告)日:2007-04-17

    申请号:US10541822

    申请日:2004-01-09

    CPC classification number: C12N9/90 C12P19/02 C12P19/24 C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在“稀释糖消化策略”(

    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof
    5.
    发明申请
    Gene sequence of L-rhamnose isomerase having new catalytic function and use thereof 有权
    具有新催化功能的L-鼠李糖异构酶的基因序列及其应用

    公开(公告)号:US20080010026A1

    公开(公告)日:2008-01-10

    申请号:US11714887

    申请日:2007-03-07

    CPC classification number: C12N9/90 C12P19/02 C12P19/24 C12Y503/01014

    Abstract: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

    Abstract translation: 在采食稀少糖的策略(图1)中,旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系,因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质(a)或(b)的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO:2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法,其特征在于,在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞,从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖,其特征在于,在单糖的总体图中,目标稀有糖的位置被理解,因此设计了允许上述蛋白质起作用的最佳生产途径。

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