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    Methods for Assaying Gene Imprinting and Methylated CpG Islands
    6.
    发明申请
    Methods for Assaying Gene Imprinting and Methylated CpG Islands 失效
    测定基因印迹和甲基化CpG群岛的方法

    公开(公告)号：US20100273658A1

    公开(公告)日：2010-10-28

    申请号：US12350159

    申请日：2009-01-07

    Applicant: ANDREW P. FEINBERG ,  LIORA STRICHMAN-ALMASHANU ,  SHAN JIANG

    Inventor： ANDREW P. FEINBERG ,  LIORA STRICHMAN-ALMASHANU ,  SHAN JIANG

    IPC: C40B20/00 ,  C12N5/073 ,  C12Q1/68 ,  C40B50/00

    CPC classification number: C12N5/0611 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/235 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2503/00 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2517/00 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/154

    Abstract: Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.

    Abstract translation: 基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。 印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。 我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。 来自种间交叉的小鼠胚胎胚芽（EG）细胞系。 我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。 然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。 我们还生成了一个包含大多数甲基化CpG岛的文库。 分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。 该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

    Methods for assaying gene imprinting and methylated CpG islands
    7.
    发明申请
    Methods for assaying gene imprinting and methylated CpG islands 失效
    测定基因印迹和甲基化CpG岛的方法

    公开(公告)号：US20050282147A1

    公开(公告)日：2005-12-22

    申请号：US11084085

    申请日：2005-03-17

    Applicant: Andrew Feinberg ,  Liora Strichman-Almashanu ,  Shan Jiang

    Inventor： Andrew Feinberg ,  Liora Strichman-Almashanu ,  Shan Jiang

    IPC: A01K67/027 ,  A61P35/00 ,  C12N5/074 ,  C12N5/10 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/00 ,  C12N5/08

    CPC classification number: C12N5/0611 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/235 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2503/00 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2517/00 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/154

    Abstract: Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.

    Abstract translation: 基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。 印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。 我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。 来自种间交叉的小鼠胚胎胚芽（EG）细胞系。 我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。 然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。 我们还生成了一个包含大多数甲基化CpG岛的文库。 分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。 该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

    Methods for assaying gene imprinting and methylated CpG islands
    8.
    发明申请
    Methods for assaying gene imprinting and methylated CpG islands 失效
    测定基因印记和甲基化CpG岛的方法

    公开(公告)号：US20020045257A1

    公开(公告)日：2002-04-18

    申请号：US09861893

    申请日：2001-05-22

    Inventor： Andrew P. Feinberg ,  Liora Strichman-almashanu

    IPC: C12N005/06

    CPC classification number: C12N5/0611 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/235 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2503/00 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2517/00 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/154

    Abstract: Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.

    Abstract translation: 基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。 印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。 我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。 来自种间交叉的小鼠胚胎胚芽（EG）细胞系。 我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。 然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。 我们还生成了一个包含大多数甲基化CpG岛的文库。 分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。 该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

    SOLID CHIMERIC ORGANS, ANIMAL MODELS HAVING SAME, PROCESS FOR PREPARING SAME, NON-TUMORIGENIC IMMORTALIZED HUMAN CELL LINES, SUSCEPTIBLE CELLS AND CYTOPATHIC MAMMALIAN VIRUSES
    9.
    发明申请
    SOLID CHIMERIC ORGANS, ANIMAL MODELS HAVING SAME, PROCESS FOR PREPARING SAME, NON-TUMORIGENIC IMMORTALIZED HUMAN CELL LINES, SUSCEPTIBLE CELLS AND CYTOPATHIC MAMMALIAN VIRUSES 无效
    固体动物器官，具有其的动物模型，制备它们的方法，非重组人免疫细胞系，可疑细胞和CYPOPATHIC MAMMALIAN病毒

    公开(公告)号：US20010007153A1

    公开(公告)日：2001-07-05

    申请号：US08876635

    申请日：1997-06-16

    Inventor： JENNIFER JUNE BROWN ,  ELAZAR RABBANI ,  JAMES J. DONEGAN ,  JAYANTA ROY-CHOWDHURY

    IPC: A01K067/00 ,  A01K067/033 ,  A01K067/027

    CPC classification number: A01K67/0271 ,  A61K2035/122 ,  C12N2503/04 ,  C12N2510/04 ,  C12N2517/00 ,  C12N2517/02

    Abstract: This invention provides useful solid chimeric organs as well as animal models having such solid chimeric organs and processes for their preparation. Such organs find significant value and use in developing new models for disease, drug and therapeutic investigations and monitoring, and in studying storage functions and processes. The solid chimeric organs are comprised of recipient cells and donor cells, the latter themselves comprising allogeneic or xenogeneic cells which are unmodified or modified to contain one or more nucleic acid segments capable of exhibiting at least one biological property, e.g. DNA synthesis, replication, promoter function, transcription, translation, reverse transcription, and the like, non-native to the donor cell. The solid chimeric organs can be prepared from recipient organs using recipient cells and implanted allogeneic or xenogeneic donor cells from non-homologous organs or tissues. Also provided by this invention are non-tumorigenic immortalized human cell lines modified by having sequences derived from hepatotrophic viruses introduced into the cells. Susceptible cells, cytopathic mammalian viruses, target cells rendered susceptible to cytopathic events, and human cells infected or transfected by noncytopathic viruses are also provided.

    Abstract translation: 本发明提供了有用的固体嵌合器官以及具有这种固体嵌合器官的动物模型及其制备方法。 这些器官在开发疾病，药物和治疗调查和监测的新模型以及研究储存功能和过程方面发现了重要的价值和用途。 固体嵌合器官由受体细胞和供体细胞组成，后者本身包含未修饰或修饰以含有一个或多个能够表现出至少一种生物学特性的核酸区段的同种异体或异种细胞。 DNA合成，复制，启动子功能，转录，翻译，逆转录等，对供体细胞是非天然的。 固体嵌合器官可以使用受体细胞和来自非同源器官或组织的植入的同种异体或异种供体细胞从受体器官制备。 本发明还提供非致瘤性永生化人细胞系，其通过将源自引入细胞的肝细胞病毒的序列修饰。 还提供易感细胞，致细胞病毒性哺乳动物病毒，对细胞病变事件变得敏感的靶细胞，以及非细胞病变病毒感染或转染的人类细胞。

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