Abstract:
The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.
Abstract:
A nerve growth promoter and an antioxidant containing a degradation product obtained by degrading a composition containing a hyaluronic acid and a protein with a protease. A would treatment agent containing an ethyl acetate extract of a degradation product obtained by degrading a composition containing a hyaluronic acid and a protein with a protease.
Abstract:
The present disclosure relates to an antimicrobial peptide analogue derived from abalone and an antimicrobial pharmaceutical composition containing the same. The antimicrobial peptide analogue according to the present disclosure is designed based on hdMolluscidin which is a peptide derived from the gill of abalone and has been designed to be commercially viable by reducing the number of amino acids. Despite the reduced number of amino acids, the designed peptide analogue exhibits very superior antimicrobial activity as well as high membrane permeability and low hemolytic activity.
Abstract:
Compositions comprising chelating agents, metal ion salts, gelling agents or a buffer, antimicrobials, antibiofilm agents and a pH adjuster or a buffer for the prevention and treatment of wound infections and food-borne diseases involving bacterial biofilms are disclosed. The anti-infective properties of a composition include reduction or killing of anaerobic/aerobic/facultative gram-negative and gram-positive wound infection associated bacteria occurring in polymicrobial biofilms. The composition may be in the form of lotion, cream, ointment, dressing, bandage, rinse, soak, gel, spray, or other suitable forms, including certain devices. Additionally, the invention offers an efficient method of delivering the formulated composition containing one or two chelating agents or chelating agents alone or in combination with a metal ion salt using either a nanoparticle or other efficient delivery systems.
Abstract:
A method of preserving a harvested cultivated food can include applying a preservative composition onto a cultivated food. The preservative composition can include an amino acid, peptide having the amino acid or protein having the amino acid. The amino acid or peptide or protein having the amino acid can be associated with a water soluble substance. The water soluble substance may facilitate application of the amino acid to the cultivated food and may contribute to the improvement of preservation of the cultivated food post-harvest. A container can be configured for protecting harvested cultivated food by having a chamber with a preservative composition located the container. A substrate can include a preservative composition located on the substrate, which can be applied to a cultivated food to increase preservation.
Abstract:
Prophylactic and/or therapeutic antipathogen agents are provided that disrupt or prevent the formation of at least one homotypic and/or heterotypic protein-protein interaction that has at least one CEA-family protein and that is involved in the establishment and colonization of a pathogen in a suitable host.
Abstract:
One aspect of the present invention is concerned with a composition containing: (A) at least 10 μg of particular substituted pyrroles per kg of dry matter; and (B) at least 100 mg per kg of dry matter of a pyranone selected from the group consisting of maltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one and combinations thereof; which composition, when dissolved in water at a dry solids content of 0.1 wt. %, exhibits: i. an absorption at 280 nm (A280) that exceeds 0.01, preferably exceeds 0.05; and ii. an absorption ratio A280/560 of at least 100, preferably of at least 200. The present composition can advantageously be used as an additive in beverages or foodstuffs to prevent or reduce light induced flavor changes. The invention also encompasses a process for the manufacture of the aforementioned composition.
Abstract:
Disclosed are methods of producing an antimicrobial solution, involving dissolving nisin in hydrochloric acid and adding an ionic chelator solution to form a base solution, heating the base solution, adding sodium lactate to the base solution, followed by adding sorbic acid to the base solution, heating the base solution, followed by adding hydrogen peroxide to the base solution, followed by adding citric acid to the base solution, followed by heating the base solution, followed by adjusting the pH of the base solution to about 5.0±0.2 with a base. Also disclosed are antimicrobial solutions and/or anti-browning solutions produced by the methods described herein. Furthermore, there are disclosed methods for reducing bacteria on produce and/or minimizing enzymatic browning of produce, involving contacting the produce with an effective bacterial reducing amount and/or an effective enzymatic browning minimizing amount of the antimicrobial solution described herein.
Abstract:
A tetrapeptide having antioxidant activity is provided. The tetrapeptide has a structure comprising, in amino acid sequence from N-terminus to C-terminus: tryptophan-X-tyrosine-X; wherein X is arginine, lysine, histidine or any positively-charged amino acid derivative such as 5-hydroxylysine, ornithine, 2,4-diamino-butyrate and 2,3-diamino-propionate. Each amino acid of the sequence or its Homo-amino acid derivative is independently of the D configuration (D-stereoisomer) or of the L configuration (L-stereoisomer), and the C-terminal comprises one selected from the group consisting of carboxyl (—COOH), and carboxamide (—CONH2). A composition stabilized to oxidation or having antioxidant activity and a method for attenuating effects of free radicals on a keratinous material are also provided.
Abstract:
Improved synthetic copolypeptide antimicrobials contain cationic amino acid residues and may be based on a blocky sequence. These antimicrobials show low mammalian toxicity and may undergo directed self-assembly. The inventive synthetic copolypeptides are useful in treatment of wounds and other infections.