公开(公告)号：US20210188924A1

公开(公告)日：2021-06-24

申请号：US17178179

申请日：2021-02-17

Applicant: Synthetic Genomics, Inc.

Inventor： Eric R. Moellering ,  Nicholas Bauman ,  Randor R. Radakovits ,  Roberto Spreafico ,  Fedor Kuzminov ,  Imad Ajjawi ,  Saheed Imam ,  Andrew Schultz ,  Kathleen Kwok ,  Moena Aqui ,  Jennifer Nominati ,  John Verruto ,  Shaun Bailey

IPC: C07K14/405 ,  C12N1/36 ,  C12N1/12 ,  C12N15/82 ,  C12P21/00 ,  C12N15/01 ,  C12P7/64 ,  A01H1/06 ,  C12R1/89

Abstract: Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

公开(公告)号：US10968259B2

公开(公告)日：2021-04-06

申请号：US15859094

申请日：2017-12-29

Applicant: Synthetic Genomics, Inc.

Inventor： Eric R. Moellering ,  Nicholas Bauman ,  Randor R. Radakovits ,  Roberto Spreafico ,  Fedor Kuzminov ,  Imad Ajjawi ,  Saheed Imam ,  Andrew Schultz ,  Kathleen Kwok ,  Moena Aqui ,  Jennifer Nominati ,  John Verruto ,  Shaun Bailey

IPC: C07K14/405 ,  C12N1/36 ,  C12N1/12 ,  C12N15/82 ,  C12P21/00 ,  C12N15/01 ,  C12P7/64 ,  A01H1/06 ,  C12R1/89

Abstract: Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

公开(公告)号：US10544424B2

公开(公告)日：2020-01-28

申请号：US15130866

申请日：2016-04-15

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Christen G. DiPetrillo ,  Jay McCarren ,  Leah Soriaga

IPC: C12N15/82 ,  C07K14/405 ,  C12N1/12 ,  C12P7/64

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated chloroplastic SRP54 genes and exhibit increased productivity with respect to wild type strains. Also provided are mutant algal strains having mutated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene. Also included are constructs and methods for attenuating or disrupting SRP54 genes.

公开(公告)号：US10266834B2

公开(公告)日：2019-04-23

申请号：US15236144

申请日：2016-08-12

Applicant: Synthetic Genomics, Inc.

Inventor： Robert C. Brown ,  Kurt I. Kamrud

IPC: C12N15/113 ,  C12N15/86 ,  C12N15/81 ,  C12N15/62 ,  C12N9/62 ,  A61K39/00 ,  A61K39/29 ,  C12N7/00 ,  C07K16/10 ,  C07K14/005 ,  C07K14/745 ,  C07K14/435 ,  C12N9/78

Abstract: The present invention provides compositions and methods for the production and delivery of recombinant double-stranded RNA molecules (dsRNA) encoding heterologous proteins, which can be useful for various therapeutic purposes as well as for the production of desired proteins. The compositions contain engineered double-stranded RNA particles (dsRPs) that can contain a double-stranded RNA molecule that can be a genome or portion of a genome, which can be enclosed in a capsid or coat protein. The dsRNA molecule also comprises an RNA sub-sequence that encodes a heterologous protein. The dsRPs can be derived from wild-type viral organisms. The delivery of the dsRPs (or DNA or RNA molecules) of the invention to an organism provides for therapeutic benefits as well as for the production of desired proteins.

公开(公告)号：US10174087B2

公开(公告)日：2019-01-08

申请号：US15299457

申请日：2016-10-20

Applicant: Synthetic Genomics, Inc.

Inventor： Jonathan E. Meuser ,  Christen G. DiPetrillo ,  Jay McCarren ,  Shaun Bailey

IPC: C12N1/12 ,  C12N15/113 ,  C12N15/11 ,  C12N15/90 ,  C07K14/405

Abstract: Mutant photosynthetic algae having increased biomass productivity are provided. The mutants have attenuated expression of violaxanthin chlorophyll a binding proteins (VCP) or fucoxanthin chlorophyll a/c binding proteins (FCP), reduced chlorophyll, higher apparent ETR(II), little to no reduction in Pmax per cell, and decreased NPQ over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting VCP or FCP genes. Also provided are methods of culturing VCP or FCP mutants for the production of biomass or other products.

公开(公告)号：US20180340165A1

公开(公告)日：2018-11-29

申请号：US16056343

申请日：2018-08-06

Applicant: Synthetic Genomics, Inc.

Inventor： J. Craig Venter ,  Hamilton O. Smith ,  Clyde A. Hutchison, III ,  Daniel G. Gibson

IPC: C12N15/10 ,  C12N15/66

Abstract: Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

公开(公告)号：US09765126B2

公开(公告)日：2017-09-19

申请号：US14506575

申请日：2014-10-03

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Moena Aqui ,  Leah Soriaga

IPC: C07K14/405 ,  C12N1/12 ,  C12N15/79 ,  C12N15/82 ,  C12P1/00 ,  C12P7/64 ,  C12P13/04 ,  C12P19/00 ,  C12P19/34 ,  C12P21/00

CPC classification number: C07K14/405 ,  C12N1/12 ,  C12N15/79 ,  C12N15/8261 ,  C12P1/00 ,  C12P7/64 ,  C12P7/649 ,  C12P13/04 ,  C12P19/00 ,  C12P19/34 ,  C12P21/00 ,  Y02A40/146 ,  Y02E50/13

Abstract: The disclosure generally relates to methods and materials for modulating cell productivity. In particular, the present disclosure provides polynucleotides encoding transcription factor proteins that when overexpressed in microorganisms result in increased in productivity, such as increased biomass productivity. Also disclosed are methods of using the genetically engineered host strains to modulate or increase productivity of host cells such as, for example, algal or heterokont cells. Genetically engineered host cells, such as algal and heterokont cells having increased biomass productivity and bioproducts derived from such host cells are also disclosed.

公开(公告)号：US09546372B2

公开(公告)日：2017-01-17

申请号：US13915522

申请日：2013-06-11

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Michele M. Champagne ,  Toby H. Richardson ,  Jun Urano

IPC: C12N15/82 ,  C12N15/79

CPC classification number: C12N15/8216 ,  C07K14/405 ,  C12N15/79

Abstract: The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.

Abstract translation: 本申请提供了新的调控元件，包括来自海洋微生物的启动子序列。 本申请进一步公开了含有这些新型调节元件的DNA构建体; 转基因细胞，转基因非人生物和含有这些新型调节元件的后代。 还公开了修饰，制备和使用调节元件的方法。 本文公开的调节元件特别适用于南非氯氰菊酯和其他微藻类。

公开(公告)号：US09528061B2

公开(公告)日：2016-12-27

申请号：US13229504

申请日：2011-09-09

Applicant: Brian G. Clement ,  Toby Richardson ,  Stuart A. Underwood

Inventor： Brian G. Clement ,  Toby Richardson ,  Stuart A. Underwood

IPC: C10L3/08 ,  C10G1/00 ,  C12P5/02 ,  C13K1/02

CPC classification number: C10L3/08 ,  C10G1/00 ,  C10G2300/1014 ,  C12P5/023 ,  C12P2201/00 ,  C13K1/02 ,  Y02E50/343 ,  Y02P30/20

Abstract: The present methods deal with the solubilization of coal and lignocellulose biomass using pyrophosphates, polyphosphates, or derivatives thereof.

Abstract translation: 本发明的方法涉及使用焦磷酸盐，多磷酸盐或其衍生物的煤和木质纤维素生物质的溶解。

公开(公告)号：US09506090B2

公开(公告)日：2016-11-29

申请号：US14033300

申请日：2013-09-20

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

IPC: C12P7/58 ,  C07D307/68 ,  C12P17/12

CPC classification number: C12P7/58 ,  C07D307/68 ,  C12P17/12

Abstract: The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

Abstract translation: 本发明提供了生产一种或多种酶途径的产物的方法。 用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中L-赖康酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。 在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。 所述方法包括作为步骤的酶和化学转化。 还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。 该方法还涉及使用以高特异性和高效率进行反应的工程化酶。 可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。 可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。