公开(公告)号：US10544424B2

公开(公告)日：2020-01-28

申请号：US15130866

申请日：2016-04-15

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Christen G. DiPetrillo ,  Jay McCarren ,  Leah Soriaga

IPC: C12N15/82 ,  C07K14/405 ,  C12N1/12 ,  C12P7/64

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated chloroplastic SRP54 genes and exhibit increased productivity with respect to wild type strains. Also provided are mutant algal strains having mutated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene. Also included are constructs and methods for attenuating or disrupting SRP54 genes.

公开(公告)号：US09765126B2

公开(公告)日：2017-09-19

申请号：US14506575

申请日：2014-10-03

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Moena Aqui ,  Leah Soriaga

IPC: C07K14/405 ,  C12N1/12 ,  C12N15/79 ,  C12N15/82 ,  C12P1/00 ,  C12P7/64 ,  C12P13/04 ,  C12P19/00 ,  C12P19/34 ,  C12P21/00

CPC classification number: C07K14/405 ,  C12N1/12 ,  C12N15/79 ,  C12N15/8261 ,  C12P1/00 ,  C12P7/64 ,  C12P7/649 ,  C12P13/04 ,  C12P19/00 ,  C12P19/34 ,  C12P21/00 ,  Y02A40/146 ,  Y02E50/13

Abstract: The disclosure generally relates to methods and materials for modulating cell productivity. In particular, the present disclosure provides polynucleotides encoding transcription factor proteins that when overexpressed in microorganisms result in increased in productivity, such as increased biomass productivity. Also disclosed are methods of using the genetically engineered host strains to modulate or increase productivity of host cells such as, for example, algal or heterokont cells. Genetically engineered host cells, such as algal and heterokont cells having increased biomass productivity and bioproducts derived from such host cells are also disclosed.

公开(公告)号：US10563232B2

公开(公告)日：2020-02-18

申请号：US16267940

申请日：2019-02-05

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Eric R. Moellering ,  Leah Soriaga ,  Moena Aqui

IPC: C12N1/12 ,  C12P7/64 ,  C07K14/405

Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

公开(公告)号：US20180155731A1

公开(公告)日：2018-06-07

申请号：US15878243

申请日：2018-01-23

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Rekha Seshadri ,  Ariel S. Schwartz ,  Leah Soriaga ,  Robert C. Brown

IPC: C12N15/79 ,  C12Q1/6876 ,  C12N15/67

CPC classification number: C12N15/79 ,  C12N15/67 ,  C12Q1/6876

Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.

公开(公告)号：US10227619B2

公开(公告)日：2019-03-12

申请号：US15210845

申请日：2016-07-14

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Eric R. Moellering ,  Leah Soriaga ,  Moena Aqui

IPC: C12N1/12 ,  C12P7/64

Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

公开(公告)号：US20170121742A1

公开(公告)日：2017-05-04

申请号：US15341998

申请日：2016-11-02

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Leah Soriaga ,  Moena Aqui ,  Eric R. Moellering

IPC: C12P7/64 ,  C12N15/11 ,  C12N15/113

Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while producing biomass at levels that are at least 45% of wild type biomass productivity under nitrogen replete conditions. Particular mutants produce at least 50% as much FAME lipid as wild type while producing at least the amount of biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

公开(公告)号：US20140220638A1

公开(公告)日：2014-08-07

申请号：US14099879

申请日：2013-12-06

Applicant: Synthetic Genomics, Inc.

Inventor： Shaun Bailey ,  Jay McCarren ,  Soyan Leung Lieberman ,  Jonathan E. Meuser ,  Anna E. Romano ,  Daniel Yee ,  Leah Soriaga ,  Robert C. Brown ,  Joseph C. Weissman ,  Roger C. Prince ,  Robert D. Nielsen ,  Ariel S. Schwartz

IPC: C12N15/82 ,  C12N15/01 ,  C12N1/12

CPC classification number: C12N15/825 ,  C07K14/405 ,  C07K14/415 ,  C12N1/12 ,  C12N1/36 ,  C12N15/01 ,  C12N15/8269

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher Pmax per unit chlorophyll with little to no reduction in Pmax per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.

Abstract translation: 提供了具有降低的叶绿素和增加的光合效率的突变的光合微生物。 突变体锁定在高光适应表型中，其中许多光适应参数特征的高光适应野生型细胞在LIHLA突变体中发现适应低光，如叶绿素降低，NPQ降低，qP升高， 更高的Ek，每单位叶绿素的Pmax更高，每个细胞的Pmax几乎不降低，并且在宽范围的光强度下具有较高的通过光系统II的电子传输速率。 本文提供了用于减弱或破坏基因的构建体用于产生具有LIHLA表型的突变体。 还提供了培养用于生产生物质或其他产品的LIHLA突变体的方法。

公开(公告)号：US11261452B2

公开(公告)日：2022-03-01

申请号：US15878243

申请日：2018-01-23

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Rekha Seshadri ,  Ariel S. Schwartz ,  Leah Soriaga ,  Robert C. Brown

IPC: C12N15/79 ,  C12N15/67 ,  C12Q1/6876

Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.

公开(公告)号：US20200172943A1

公开(公告)日：2020-06-04

申请号：US16790631

申请日：2020-02-13

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Eric R. Moellering ,  Leah Soriaga ,  Moena Aqui

IPC: C12P7/64 ,  C12N1/12 ,  C07K14/405

Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

公开(公告)号：US20200157558A1

公开(公告)日：2020-05-21

申请号：US16773233

申请日：2020-01-27

Applicant: Synthetic Genomics, Inc.

Inventor： Christen G. DiPetrillo ,  Jay McCarren ,  Leah Soriaga

IPC: C12N15/82 ,  C12P7/64 ,  C12N1/12 ,  C07K14/405

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated chloroplastic SRP54 genes and exhibit increased productivity with respect to wild type strains. Also provided are mutant algal strains having mutated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene. Also included are constructs and methods for attenuating or disrupting SRP54 genes.