公开(公告)号：US20200157558A1

公开(公告)日：2020-05-21

申请号：US16773233

申请日：2020-01-27

Applicant: Synthetic Genomics, Inc.

Inventor： Christen G. DiPetrillo ,  Jay McCarren ,  Leah Soriaga

IPC: C12N15/82 ,  C12P7/64 ,  C12N1/12 ,  C07K14/405

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated chloroplastic SRP54 genes and exhibit increased productivity with respect to wild type strains. Also provided are mutant algal strains having mutated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene. Also included are constructs and methods for attenuating or disrupting SRP54 genes.

公开(公告)号：US20160304896A1

公开(公告)日：2016-10-20

申请号：US15130866

申请日：2016-04-15

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Christen G. DiPetrillo ,  Jay McCarren ,  Leah Soriaga

IPC: C12N15/82 ,  C07K14/405 ,  C12P7/64 ,  C12N1/12

CPC classification number: C12N15/8269 ,  C07K14/405 ,  C12N1/12 ,  C12N15/8218 ,  C12N15/8247 ,  C12P7/64 ,  C12P7/649 ,  Y02E50/13

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated chloroplastic SRP54 genes and exhibit increased productivity with respect to wild type strains. Also provided are mutant algal strains having mutated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene. Also included are constructs and methods for attenuating or disrupting SRP54 genes.

Abstract translation: 提供了具有降低的叶绿素和增加的光合效率的突变的光合微生物。 突变株具有突变的叶绿体SRP54基因，并且相对于野生型菌株表现出增加的生产力。 还提供具有突变的胞质SRP54基因的突变型藻类菌株。 本文提供使用在SRP54基因中具有突变的菌株来生产生物质和其它产物如脂质的方法。 还包括用于减弱或破坏SRP54基因的构建体和方法。

公开(公告)号：US10544424B2

公开(公告)日：2020-01-28

申请号：US15130866

申请日：2016-04-15

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Christen G. DiPetrillo ,  Jay McCarren ,  Leah Soriaga

IPC: C12N15/82 ,  C07K14/405 ,  C12N1/12 ,  C12P7/64

Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated chloroplastic SRP54 genes and exhibit increased productivity with respect to wild type strains. Also provided are mutant algal strains having mutated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene. Also included are constructs and methods for attenuating or disrupting SRP54 genes.

公开(公告)号：US10174087B2

公开(公告)日：2019-01-08

申请号：US15299457

申请日：2016-10-20

Applicant: Synthetic Genomics, Inc.

Inventor： Jonathan E. Meuser ,  Christen G. DiPetrillo ,  Jay McCarren ,  Shaun Bailey

IPC: C12N1/12 ,  C12N15/113 ,  C12N15/11 ,  C12N15/90 ,  C07K14/405

Abstract: Mutant photosynthetic algae having increased biomass productivity are provided. The mutants have attenuated expression of violaxanthin chlorophyll a binding proteins (VCP) or fucoxanthin chlorophyll a/c binding proteins (FCP), reduced chlorophyll, higher apparent ETR(II), little to no reduction in Pmax per cell, and decreased NPQ over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting VCP or FCP genes. Also provided are methods of culturing VCP or FCP mutants for the production of biomass or other products.

公开(公告)号：US20170114107A1

公开(公告)日：2017-04-27

申请号：US15299457

申请日：2016-10-20

Applicant: Synthetic Genomics, Inc.

Inventor： Jonathan E. Meuser ,  Christen G. DiPetrillo ,  Jay McCarren ,  Shaun Bailey

IPC: C07K14/405 ,  C12N15/90 ,  C12N15/113 ,  C12N1/12

CPC classification number: C07K14/405 ,  C12N1/12 ,  C12N15/113 ,  C12N15/902 ,  C12N2800/80

Abstract: Mutant photosynthetic algae having increased biomass productivity are provided. The mutants have attenuated expression of violaxanthin chlorophyll a binding proteins (VCP) or fucoxanthin chlorophyll a/c binding proteins (FCP), reduced chlorophyll, higher apparent ETR(II), little to no reduction in Pmax per cell, and decreased NPQ over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting VCP or FCP genes. Also provided are methods of culturing VCP or FCP mutants for the production of biomass or other products.

公开(公告)号：US20160186147A1

公开(公告)日：2016-06-30

申请号：US14970458

申请日：2015-12-15

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： Kyle C. Cady ,  E. Magda Barbu ,  Christen G. DiPetrillo

IPC: C12N7/00 ,  C12N9/22 ,  C12P19/34 ,  C12N15/70

CPC classification number: C12N7/00 ,  C12N9/22 ,  C12N2710/16121 ,  C12N2795/10221 ,  C12N2795/10222 ,  C12N2795/10321 ,  C12N2795/10322 ,  C12N2795/14121 ,  C12N2795/14122 ,  C12P19/34 ,  C12Y301/00

Abstract: The present disclosure relates to a method of in vitro engineering of nucleic acids. This disclosure further relates to in vitro engineering of viral genomes and to the improvement of viral properties by in vitro genomic engineering of viral genomes. Specifically, the disclosure relates to in vitro viral genomic digestion using RNA-guided Cas9, the assembly of a recombinant genome by the insertion of a DNA or RNA fragment into the digested viral genome and transformation of a host cell with the recombinant genome. This method also related to in vitro engineering for error correction of nucleic acids.

Abstract translation: 本公开涉及核酸的体外工程方法。 本公开还涉及病毒基因组的体外工程和通过病毒基因组的体外基因组工程来改善病毒性质。 具体地，本公开涉及使用RNA引导的Cas9的体外病毒基因组消化，通过将DNA或RNA片段插入消化的病毒基因组并用重组基因组转化宿主细胞来重组基因组的装配。 该方法也涉及核酸纠错的体外工程。