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    METHODS AND DEVICES FOR NUCLEIC ACID EXTRACTION USING EPITACHOPHORESIS
    3.
    发明公开
    METHODS AND DEVICES FOR NUCLEIC ACID EXTRACTION USING EPITACHOPHORESIS 审中-公开

    公开(公告)号：US20240318164A1

    公开(公告)日：2024-09-26

    申请号：US18577938

    申请日：2022-07-21

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Pantea GHEIBI ,  Keynttisha JEFFERSON ,  Vladimira DATINSKA

    IPC分类号： C12N15/10 ,  C12N9/64

    CPC分类号： C12N15/101 ,  C12N9/6424 ,  C12Y304/21064

    摘要： Epitachophoresis (ETP) methods and systems described herein allow for efficient and improved extraction of DNA and RNA molecules from a biological sample. The extraction may involve fragmenting nucleic acid molecules to smaller sizes and then running the fragmented sample through an ETP device. The fragmentation improves the extraction of nucleic acid molecules when using a gel with ETP. Fragmentation may also reduce extraction of undesired ribosomal RNA with gel ETP. Nucleic acid molecules are fragmented for preparing a library, and therefore the fragmentation of nucleic acid molecules before extraction rather than after extraction does not negatively impact library prep. In order to facilitate fragmentation, nucleic acid molecules may be treated so that the nucleic acid molecules are not protected from fragmentation.

    Long lifetime alpha-hemolysin nanopores
    4.
    发明授权
    Long lifetime alpha-hemolysin nanopores 有权

    公开(公告)号：US12091714B2

    公开(公告)日：2024-09-17

    申请号：US18171969

    申请日：2023-02-21

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Mark Ambroso ,  Timothy Craig ,  Matthew DiPietro ,  Corissa Harris ,  Marshall Porter

    IPC分类号： C07K14/00 ,  A61K38/02 ,  C07K14/31 ,  C12N15/01 ,  C12Q1/6869

    CPC分类号： C12Q1/6869 ,  A61K38/02 ,  C07K14/31 ,  C12N15/01 ,  C07K14/00

    摘要： Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A, and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L135I in the mature alpha-hemolysin amino acid sequence. The α-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having an increased nanopore lifetime. Further, provided are variants that, in addition to providing increased lifetime, provide a decreased time-to-thread. Hence, the variants provided herein both increase nanopore lifetime and improve efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants.

    Nanopore arrays
    9.
    发明授权
    Nanopore arrays 有权

    公开(公告)号：US11988659B2

    公开(公告)日：2024-05-21

    申请号：US16948372

    申请日：2020-09-15

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Roger J. A. Chen ,  David J. Fullagar

    IPC分类号： G01N33/487 ,  G11C7/00 ,  G11C16/34

    CPC分类号： G01N33/48721 ,  G11C7/00 ,  G11C16/34

    摘要： A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.