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26 条记录 (耗时 0.018 秒)

    Adjustable bilayer capacitance structure for biomedical devices
    1.
    发明授权
    Adjustable bilayer capacitance structure for biomedical devices 有权

    公开(公告)号：US10976302B2

    公开(公告)日：2021-04-13

    申请号：US16868457

    申请日：2020-05-06

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Roger J. A. Chen ,  Jennifer Hovis ,  Hui Tian

    IPC分类号： G01N33/487

    摘要： A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

    Electrical enhancement of bilayer formation
    3.
    发明授权
    Electrical enhancement of bilayer formation 有权

    公开(公告)号：US10465240B2

    公开(公告)日：2019-11-05

    申请号：US15085713

    申请日：2016-03-30

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Ashraf Wahba ,  William Nielsen ,  J. William Maney, Jr. ,  Kevin Aliado ,  Kyle Umeda ,  Roger J. A. Chen ,  Jing Luo

    IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  G01N33/487

    摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

    Systems for forming a nanopore in a membrane
    4.
    发明授权
    Systems for forming a nanopore in a membrane 有权

    公开(公告)号：US11092589B2

    公开(公告)日：2021-08-17

    申请号：US16516166

    申请日：2019-07-18

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Roger J. A. Chen ,  Randall Davis

    IPC分类号： B81B1/00 ,  G01N33/483 ,  G01N33/487 ,  G01N15/12 ,  C12Q1/6869 ,  B82Y5/00

    摘要： A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

    Nanopore-based sequencing using voltage mode with hybrid mode stimuli
    5.
    发明授权
    Nanopore-based sequencing using voltage mode with hybrid mode stimuli 有权

    公开(公告)号：US11029306B2

    公开(公告)日：2021-06-08

    申请号：US16297428

    申请日：2019-03-08

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Roger J. A. Chen ,  Hui Tian ,  J. William Maney, Jr.

    IPC分类号： G01N33/487 ,  C12Q1/6869 ,  G01N27/447

    摘要： A liquid voltage is applied to a first side of a lipid bilayer. The liquid voltage comprises a tag-reading period with a tag-reading voltage that tends to capture a tag into a nanopore in the lipid bilayer and an open-channel period with an open-channel voltage that tends to repel the tag. A pre-charging voltage source is connected to an integrating capacitor and a working electrode on a second side of the lipid bilayer during a pre-charging time period, such that the integrating capacitor and the working electrode are charged to a pre-charging voltage. The pre-charging voltage source is disconnected from the integrating capacitor and the working electrode during an integrating time period, such that a voltage of the integrating capacitor and a voltage of the working electrode may vary as a current flows through the nanopore. The pre-charging time period overlaps with a beginning portion of the tag-reading period.

    Nucleic acid sequencing using tags
    8.
    发明授权
    Nucleic acid sequencing using tags 有权

    公开(公告)号：US11499190B2

    公开(公告)日：2022-11-15

    申请号：US16888434

    申请日：2020-05-29

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Randall W. Davis ,  Roger J. A. Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

    IPC分类号： C12Q1/6869 ,  G01N33/487 ,  G01N27/447

    摘要： This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

    Exporting measurements of nanopore arrays
    10.
    发明授权
    Exporting measurements of nanopore arrays 有权

    公开(公告)号：US10429375B2

    公开(公告)日：2019-10-01

    申请号：US16172633

    申请日：2018-10-26

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Roger J. A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

    IPC分类号： G01N27/447 ,  G01N27/22 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

    摘要： A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.