公开(公告)号：US20210310064A1

公开(公告)日：2021-10-07

申请号：US17304455

申请日：2021-06-21

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  J. William Maney, JR. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US11891661B2

公开(公告)日：2024-02-06

申请号：US18068620

申请日：2022-12-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N27/327 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721 ,  C12Q1/6869 ,  C12Q2565/607

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US11530443B2

公开(公告)日：2022-12-20

申请号：US16892212

申请日：2020-06-03

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N27/327 ,  C12Q1/6869 ,  G01N33/487

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20200032333A1

公开(公告)日：2020-01-30

申请号：US16578189

申请日：2019-09-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  J. William Maney, JR. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US20190153506A1

公开(公告)日：2019-05-23

申请号：US16260387

申请日：2019-01-29

申请人： Roche Sequencing Solutions, Inc.

发明人： Ashraf Wahba

IPC分类号： C12Q1/68 ,  G01N33/487 ,  G01R27/26 ,  C12Q1/6869

摘要： A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.

公开(公告)号：US20240229127A1

公开(公告)日：2024-07-11

申请号：US18433151

申请日：2024-02-05

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20230120047A1

公开(公告)日：2023-04-20

申请号：US18068620

申请日：2022-12-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20200291468A1

公开(公告)日：2020-09-17

申请号：US16892212

申请日：2020-06-03

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US10465240B2

公开(公告)日：2019-11-05

申请号：US15085713

申请日：2016-03-30

申请人： Roche Sequencing Solutions, Inc.

发明人： Ashraf Wahba ,  William Nielsen ,  J. William Maney, Jr. ,  Kevin Aliado ,  Kyle Umeda ,  Roger J. A. Chen ,  Jing Luo

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  G01N33/487

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US11814676B2

公开(公告)日：2023-11-14

申请号：US17304455

申请日：2021-06-21

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  J. William Maney, Jr. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N33/48 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.