公开(公告)号：US20240035083A1

公开(公告)日：2024-02-01

申请号：US18482807

申请日：2023-10-06

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  J. William Maney, JR. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US20220291192A1

公开(公告)日：2022-09-15

申请号：US17754138

申请日：2020-09-22

申请人： Roche Sequencing Solutions, Inc.

发明人： Matthew DiPietro ,  Damion Engelbart ,  Andrew Liu ,  William Nielsen ,  Janusz B. Wojtowicz ,  Robert A. Yuan

IPC分类号： G01N33/487

摘要： A consumable device used in a nanopore based sequencing system can include a nanopore chip, a flow cell with one or more flow channels, and a flow cell cover. A fluidic interface can be used to deliver fluid to the flow cell. The fluid interface can include a flow cell boss and the flow cell cover can include a receptacle for receiving the flow cell boss. A dispense tip can be used to introduce fluid into the flow cell through the flow cell boss.

公开(公告)号：US20210310064A1

公开(公告)日：2021-10-07

申请号：US17304455

申请日：2021-06-21

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  J. William Maney, JR. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US11814676B2

公开(公告)日：2023-11-14

申请号：US17304455

申请日：2021-06-21

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  J. William Maney, Jr. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N33/48 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US11041198B2

公开(公告)日：2021-06-22

申请号：US16578189

申请日：2019-09-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  J. William Maney, Jr. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N27/32 ,  C12Q1/6869 ,  G01N33/487

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US11891661B2

公开(公告)日：2024-02-06

申请号：US18068620

申请日：2022-12-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N27/327 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721 ,  C12Q1/6869 ,  C12Q2565/607

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US11530443B2

公开(公告)日：2022-12-20

申请号：US16892212

申请日：2020-06-03

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J. A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： G01N27/327 ,  C12Q1/6869 ,  G01N33/487

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20200032333A1

公开(公告)日：2020-01-30

申请号：US16578189

申请日：2019-09-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  J. William Maney, JR. ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US20240229127A1

公开(公告)日：2024-07-11

申请号：US18433151

申请日：2024-02-05

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  G01N33/48721

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20230120047A1

公开(公告)日：2023-04-20

申请号：US18068620

申请日：2022-12-20

申请人： Roche Sequencing Solutions, Inc.

发明人： Kevin Aliado ,  Roger J.A. Chen ,  Jing Luo ,  William Nielsen ,  Kyle Umeda ,  Ashraf Wahba

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.