Invention Grant
- Patent Title: Electrical enhancement of bilayer formation
-
Application No.: US15085713Application Date: 2016-03-30
-
Publication No.: US10465240B2Publication Date: 2019-11-05
- Inventor: Ashraf Wahba , William Nielsen , J. William Maney, Jr. , Kevin Aliado , Kyle Umeda , Roger J. A. Chen , Jing Luo
- Applicant: Roche Sequencing Solutions, Inc.
- Applicant Address: US CA Pleasanton
- Assignee: Roche Sequencing Solutions, Inc.
- Current Assignee: Roche Sequencing Solutions, Inc.
- Current Assignee Address: US CA Pleasanton
- Agency: Roche Sequencing Solutions, Inc.
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6869 ; G01N33/487

Abstract:
A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.
Public/Granted literature
- US20170283867A1 ELECTRICAL ENHANCEMENT OF BILAYER FORMATION Public/Granted day:2017-10-05
Information query