公开(公告)号：US11499190B2

公开(公告)日：2022-11-15

申请号：US16888434

申请日：2020-05-29

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Randall W. Davis ,  Roger J. A. Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

IPC: C12Q1/6869 ,  G01N33/487 ,  G01N27/447

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

公开(公告)号：US20190382833A1

公开(公告)日：2019-12-19

申请号：US15849581

申请日：2017-12-20

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Randall W. Davis ,  Roger J.A. Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N33/487

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

公开(公告)号：US10822650B2

公开(公告)日：2020-11-03

申请号：US15469799

申请日：2017-03-27

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N33/487

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

公开(公告)号：US11479584B2

公开(公告)日：2022-10-25

申请号：US16867891

申请日：2020-05-06

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US10683331B2

公开(公告)日：2020-06-16

申请号：US16375140

申请日：2019-04-04

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US20180030524A1

公开(公告)日：2018-02-01

申请号：US15469799

申请日：2017-03-27

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

IPC: C12Q1/68

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

公开(公告)号：US12043648B2

公开(公告)日：2024-07-23

申请号：US17934054

申请日：2022-09-21

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

CPC classification number: C07K14/31 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.