公开(公告)号：US20240309322A1

公开(公告)日：2024-09-19

申请号：US18575385

申请日：2022-07-06

申请人： KEIO UNIVERSITY

发明人： Iki SONN ,  Hideyuki OKANO ,  Hirotaka WATANABE ,  Satoru MORIMOTO

IPC分类号： C12N5/079 ,  C12N15/85

CPC分类号： C12N5/0622 ,  C12N15/85 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/415 ,  C12N2506/45 ,  C12N2800/107

摘要： Provided is a method for producing microglia, including: Step (S1) of inducing differentiation of hemangioblasts to obtain microglial progenitor cells; and Step (S2) of inducing differentiation of the microglial progenitor cells to obtain microglia, in which, in the step of obtaining microglial progenitor cells, expression of PU.1 transcription factor encoded by an exogenous gene is induced, and culture is carried out in the presence of FGF2, SCF, IL-3, IL-6, VEGF, and Wnt inhibitor.

公开(公告)号：US20240151712A1

公开(公告)日：2024-05-09

申请号：US18550496

申请日：2022-03-18

申请人： KEIO UNIVERSITY

发明人： Hiroko ISHII ,  Hideyuki OKANO ,  Takahiro KONDO ,  Yuta SATO

IPC分类号： G01N33/50 ,  C12N5/071 ,  C12N15/86

CPC分类号： G01N33/5082 ,  C12N5/0697 ,  C12N15/86 ,  G01N33/5038 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2501/727 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2750/14143

摘要： A method for producing a brain organoid having an aggregated tau protein including (a) culturing pluripotent stem cells in the presence of a SMAD inhibitor to form an embryoid body, (b) embedding the embryoid body in an extracellular matrix and three-dimensionally culturing the embryoid body in the presence of a SMAD inhibitor and a GSK3β inhibitor to form an organoid that includes neural precursor cells, (c) extracting the organoid from the extracellular matrix and suspension-culturing the organoid in the presence of LIF to form a brain organoid, (d) forcing the brain organoid to express a mutant MAPT gene, and (e) further suspension-culturing the brain organoid after (d) to obtain a brain organoid having an aggregated tau protein.

公开(公告)号：US20240124836A1

公开(公告)日：2024-04-18

申请号：US18391763

申请日：2023-12-21

申请人： KEIO UNIVERSITY

发明人： Minoru Ko

IPC分类号： C12N5/0793 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/0797 ,  C12N5/16

CPC分类号： C12N5/0619 ,  C12N5/0606 ,  C12N5/0623 ,  C12N5/067 ,  C12N5/0672 ,  C12N5/16 ,  C12N2501/60 ,  C12N2501/65 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00

摘要： Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.

公开(公告)号：US11957475B2

公开(公告)日：2024-04-16

申请号：US16300631

申请日：2017-05-15

申请人： Keio University

发明人： Junichi Ushiba ,  Fumio Liu ,  Kenichi Takasaki ,  Atsuko Nishimoto ,  Miho Hiramoto ,  Katsuhiro Mizuno ,  Meigen Liu ,  Toshiyuki Fujiwara

IPC分类号： A61B5/372 ,  A61B5/00 ,  A61B5/316 ,  A61B5/375 ,  A61B5/377 ,  A61F2/72 ,  G06F3/01

CPC分类号： A61B5/375 ,  A61B5/316 ,  A61B5/377 ,  A61B5/4848 ,  A61F2/72 ,  G06F3/015 ,  A61B2505/09

摘要： A biometric information processing device (1) includes a brain wave detecting unit (10), a control unit (20), and a movement assisting unit (30). The brain wave detecting unit (10) detects biometric information in at least one brain region from among a plurality of brain regions that are selectable in accordance with a body part that is a target of function recovery or function improvement. The control unit (20) determines, based on the detected biometric information, at least one activity state in the brain including the location of the brain region(s) that is (are) activated in a subject while attempting to move the body part, and an activation level of such activated brain region(s). When the control unit (20) determines that the at least one activated state of the brain satisfies a predetermined condition, the movement assisting unit (30) executes a predetermined motion to assist movement of the body part.

公开(公告)号：US20240011973A1

公开(公告)日：2024-01-11

申请号：US18039534

申请日：2021-12-03

申请人： KEIO UNIVERSITY ,  JSR CORPORATION

发明人： Toshiro SATO ,  Shinya SUGIMOTO ,  Kazuya ARAI

IPC分类号： G01N33/50 ,  C12N5/071

CPC分类号： G01N33/5082 ,  C12N5/0697 ,  G01N33/502 ,  C12N2533/90 ,  C12N2500/38 ,  C12N2501/415 ,  C12N2501/115 ,  C12N2501/105

摘要： A method for producing a two-dimensional small intestinal organoid having a villus structure, the method including a step 1 of culturing a cell derived from a small intestinal epithelium in an extracellular matrix to obtain a three-dimensional small intestinal organoid, a step 2 of dispersing the three-dimensional small intestinal organoid and monolayer culturing on the extracellular matrix to obtain a two-dimensional small intestinal organoid, and a step 3 of further culturing the two-dimensional small intestinal organoid while letting a culture medium of the two-dimensional small intestinal organoid to flow so that the two-dimensional small intestinal organoid forms a villus structure.

公开(公告)号：US20230390459A1

公开(公告)日：2023-12-07

申请号：US18034645

申请日：2021-10-28

申请人： KEIO UNIVERSITY ,  Jiksak Bioengineering Inc.

发明人： Shinsuke SHIBATA ,  Hiroo KIMURA ,  Takayuki NISHIJIMA ,  Tomoko SHINDO ,  Hideyuki OKANO ,  Jiro KAWADA ,  Sayaka TOITA

IPC分类号： A61L27/38

CPC分类号： A61L27/3878 ,  A61L27/3895 ,  A61L27/383

摘要： The present invention relates to an axon bundle obtained by a method of culturing a nerve cell aggregation having one spheroid and an axon bundle extending from the spheroid



wherein the nerve cell aggregation consisting of a plurality of neurons having a cell body and an axon obtained by a method comprising the following step:
supplying a culture medium to one first chamber, one second chamber and one channel having the length of at least 1 mm, the width of 100 to 150 μm and the height of 100 to 200 μm, which connecting said first chamber and second chamber, wherein said first chamber, second chamber, and channel are contained in one of the modules disposed in a culture plate,
seeding the first chamber with a nerve cell derived from a stem cell or a spheroid of primary cultured nerve cells; and
culturing said nerve cell, thereby growing an axon bundle from said spheroid and extending them into said channel,
wherein the length of the axon bundle obtained is 1 mm or more, and the diameter of the axon bundles is 50 μm or more.

公开(公告)号：US11834681B2

公开(公告)日：2023-12-05

申请号：US16824839

申请日：2020-03-20

申请人： KEIO UNIVERSITY

发明人： Toshiro Sato ,  Mami Matano

IPC分类号： C12N5/09

CPC分类号： C12N5/0693 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/415 ,  C12N2501/998

摘要： A cell culture medium for culturing organoid containing at least two types of components selected from the group consisting of insulin-like growth factor 1 (IGH1), fibroblast growth factor 2 (FGF2) and epiregulin (EREG), and at least one type of component among the following components i (to III); i) Wnt agonist, ii) bone morphogenetic protein (BMP) inhibitor, and iii) transforming growth factor-β (TGIF-β) inhibitor.

公开(公告)号：US20230310554A1

公开(公告)日：2023-10-05

申请号：US18023202

申请日：2021-08-27

申请人： KEIO UNIVERSITY ,  PUBLIC UNIVERSITY CORPORATION NARA MEDICAL UNIVERSITY ,  KIMIGAFUCHI GAKUEN

发明人： Kazuaki TAGUCHI ,  Yuto SUZUKI ,  Kazuaki MATSUMOTO ,  Hiromi SAKAI ,  Masaki OTAGIRI

IPC分类号： A61K38/42 ,  A61P39/02 ,  A61K9/127

CPC分类号： A61K38/42 ,  A61P39/02 ,  A61K9/127

摘要： The present invention is intended to provide a medicament for cyanide poisoning, hydrogen sulfide poisoning, and azide poisoning, wherein the medicament does not cause side effects, is excellent in immediate effectiveness, and has a high antidotal effect. Specifically, the present invention relates to a medicament for cyanide poisoning, hydrogen sulfide poisoning, and azide poisoning, wherein the medicament comprises, as an active ingredient(s), one or more selected from the group consisting of a heme protein capable of binding to cyanide ions (CN−), hydrogen sulfide ions (HS−) and azide ions (N3−), a substance containing a heme protein capable of binding to CN−, HS− and N3−, and a heme derivative capable of binding to CN−, HS− and N3−. More specifically, the present invention relates to a medicament or a pharmaceutical composition for the treatment or prophylaxis of cyanide poisoning, hydrogen sulfide poisoning, and azide poisoning.

公开(公告)号：US20230281475A1

公开(公告)日：2023-09-07

申请号：US18019291

申请日：2020-08-03

申请人： ANA HOLDINGS INC. ,  KEIO UNIVERSITY

发明人： Ayako Matsumoto ,  Yoshiaki Miyamoto

IPC分类号： G06N5/022

CPC分类号： G06N5/022

摘要： There is provided the turbulence prediction system or the turbulence prediction method that predicts a zone in a prediction area where the possibility of the occurrence of a turbulence is high at a determination time point. The problem is solved by the turbulence prediction system or the turbulence prediction method that makes a plurality of pieces of turbulence prediction pattern data regarding an arbitrary meteorological parameter that is made based on meteorological data regarding the arbitrary meteorological parameter at a zone where a turbulence occurred in the past, makes determination-purposed meteorological data based on meteorological data regarding the arbitrary meteorological parameter for the prediction area at the determination time point, calculates a portion having a high degree of similarity between the determination-purposed meteorological data and the plurality of pieces of turbulence prediction pattern data, and determines the location having the high degree of similarity as a zone having a high possibility of the occurrence of a turbulence in the prediction area.

公开(公告)号：US11712451B2

公开(公告)日：2023-08-01

申请号：US16162546

申请日：2018-10-17

申请人： KEIO UNIVERSITY

发明人： Yumiko Matsubara ,  Yasuo Ikeda ,  Keiichi Tozawa ,  Yukako Uruga ,  Masaki Yazawa ,  Taisuke Mori ,  Kazuo Kishi

IPC分类号： A61K35/19 ,  A61P17/02

CPC分类号： A61K35/19 ,  A61P17/02

摘要： An object of the present invention is to provide, for example, a more practical wound healing accelerator that more effectively accelerates wound healing. More specifically, a feature of the present invention is to provide, for example, a more practical wound healing accelerator that is easily obtained in a larger amount than that of peripheral blood platelet and has a better wound healing effect than that of peripheral blood platelet. The present invention employs a platelet-like cell population coexpressing one or more platelet surface markers and one or more mesenchymal cell surface markers. A wound healing accelerator containing the platelet-like cell population is a more practical wound healing accelerator that more effectively accelerates wound healing. The platelet-like cell population is easily obtained in a larger amount than that of peripheral blood platelet and has a better wound healing effect than that of peripheral blood platelet.