公开(公告)号：US20030202546A1

公开(公告)日：2003-10-30

申请号：US10137262

申请日：2002-04-30

申请人： The Regents of the University of California

发明人： Frederic V. Hartemann ,  Hector A. Baldis ,  Chris P.J. Barty ,  David J. Gibson ,  Bernhard Rupp

IPC分类号： H01S003/30

CPC分类号： G03F7/70008 ,  H05G2/00

摘要： A femtosecond laser-electron X-ray source. A high-brightness relativistic electron injector produces an electron beam pulse train. A system accelerates the electron beam pulse train. The femtosecond laser-electron X-ray source includes a high intra-cavity power, mode-locked laser and an x-ray optics system.

摘要翻译： 飞秒激光电子X射线源。 高亮度相对论电子注入器产生电子束脉冲串。 系统加速电子束脉冲串。 飞秒激光电子X射线源包括高腔体功率，锁模激光器和x射线光学系统。

公开(公告)号：US20030150375A1

公开(公告)日：2003-08-14

申请号：US10253130

申请日：2002-09-23

申请人： The Regents of the University of California

发明人： Brent W. Segelke ,  Bernhard Rupp ,  Heike I. Krupka

IPC分类号： C30B007/00 ,  C30B021/02 ,  C30B028/06

CPC分类号： B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00369 ,  B01J2219/00542 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00756 ,  C30B7/00 ,  C30B29/58 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  C40B70/00 ,  Y10S117/90 ,  Y10T117/1068 ,  Y10T117/1072 ,  Y10T117/1088

摘要： An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.

摘要翻译： 一种自动化的大分子结晶筛选系统，其中产生多种试剂混合物。 使用与样品组合的试剂混合物产生多个分析板。 孵育分析板以促进晶体的生长。 制作晶体的图像。 关于通过X射线晶体学分析晶体的适用性来分析图像。 基于通过X射线晶体学分析晶体的预期适用性产生试剂混合物的设计。 使用该设计产生第二多种试剂组分的混合物，并且将第二多种试剂混合物用于第二轮自动化大分子结晶筛选。 在一个实施方案中，多种试剂混合物通过试剂组分的随机选择产生。

公开(公告)号：US20040033181A1

公开(公告)日：2004-02-19

申请号：US10218764

申请日：2002-08-14

申请人： The Regents of the University of California

发明人： Brent W. Segelke ,  Heike I. Krupka ,  Bernhard Rupp

IPC分类号： B01D009/00

CPC分类号： C30B7/00 ,  B01L9/54 ,  C30B29/58 ,  G01N2001/4027 ,  Y10S117/901 ,  Y10T117/1024

摘要： A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

摘要翻译： 用于结晶蛋白浓度预筛选的试剂盒包括多个小瓶，多个预先选择的试剂和多个样品板。 不同浓度的溶液中的试剂和相应的蛋白质样品数量放在样品板上。 将含有试剂和样品的样品板孵育。 孵育后，检查样品板以确定哪个样品浓度太低，样品浓度太高。 选择和使用最适合蛋白质结晶的样品浓度。