公开(公告)号：US20250027167A1

公开(公告)日：2025-01-23

申请号：US18740012

申请日：2024-06-11

Applicant: The Johns Hopkins University

Inventor： Chetan Bettegowda ,  Kenneth W. Kinzler ,  Bert Vogelstein ,  Yuxuan Wang ,  Luis Diaz ,  Nickolas Papadopoulos

IPC: C12Q1/6886

Abstract: As cell-free DNA from brain and spinal cord tumors cannot usually be detected in the blood, we assessed the cerebrospinal fluid (CSF) that bathes the CNS for tumor DNA, here termed CSF-tDNA. The results suggest that CSF-tDNA could be useful for the management of patients with primary tumors of the brain or spinal cord.

公开(公告)号：US20250019755A1

公开(公告)日：2025-01-16

申请号：US18899799

申请日：2024-09-27

Applicant: The Johns Hopkins University

Inventor： Nickolas Papadopoulos ,  Kenneth W. Kinzler ,  Bert Vogelstein ,  Joshua David Cohen

IPC: C12Q1/6855 ,  C12Q1/6876

Abstract: Provided herein are systems, kits, compositions and methods for sequencing library preparation and sequencing workflow (e.g., for the identification of mutations). In certain embodiments, provides herein systems and methods to identically barcode both strands of templates, and PCR-based enrichment of each strand that does not require hybridization capture.

公开(公告)号：US20240344137A1

公开(公告)日：2024-10-17

申请号：US18518055

申请日：2023-11-22

Applicant: The Johns Hopkins University

Inventor： Tobias Sjoblom ,  Sian Jones ,  D. Williams Parsons ,  Laura D. Wood ,  Jimmy Cheng-Ho Lin ,  Thomas Barber ,  Diana Mandelker ,  Bert Vogelstein ,  Kenneth W. Kinzler ,  Victor E. Velculesu

IPC: C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156

Abstract: Analysis of 13,023 genes in 11 breast and 11 colorectal cancers revealed that individual tumors accumulate an average of ˜90 mutant genes but that only a subset of these contribute to the neoplastic process. Using stringent criteria to delineate this subset, we identified 189 genes (average of 11 per tumor) that were mutated at significant frequency. The vast majority of these genes were not known to be genetically altered in tumors and are predicted to affect a wide range of cellular functions, including transcription, adhesion, and invasion. These data define the genetic landscape of two human cancer types, provide new targets for diagnostic and therapeutic intervention and monitoring.

公开(公告)号：US12116628B2

公开(公告)日：2024-10-15

申请号：US17483537

申请日：2021-09-23

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Nickolas Papadopoulos ,  Isaac A. Kinde

IPC: C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6883

CPC classification number: C12Q1/6869 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q1/6827 ,  C12Q2535/122 ,  C12Q2537/16 ,  C12Q2537/165 ,  C12Q2545/114

Abstract: Massively parallel sequencing of cell-free, maternal plasma DNA was recently demonstrated to be a safe and effective screening method for fetal chromosomal aneuploidies. Here, we report an improved sequencing method achieving significantly increased throughput and decreased cost by replacing laborious sequencing library preparation steps with PCR employing a single primer pair. Using this approach, samples containing as little as 4% trisomy 21 DNA could be readily distinguished from euploid samples.

公开(公告)号：US20240002541A1

公开(公告)日：2024-01-04

申请号：US18039403

申请日：2021-12-01

Applicant: The Johns Hopkins University

Inventor： Sarah DiNapoli ,  Jacqueline Douglass ,  Emily Han-Chung Hsiue ,  Michael S. Hwang ,  Kenneth W. Kinzler ,  Maximilian Konig ,  Brian J. Mog ,  Nickolas Papadopoulos ,  Andrew M. Pardoll ,  Suman Paul ,  Alexander H. Pearlman ,  Bert Vogelstein ,  Shibin Zhou

IPC: C07K16/46 ,  A61P35/00

CPC classification number: C07K16/468 ,  A61P35/00 ,  C07K2317/565 ,  C07K2317/31

Abstract: This document relates to methods and materials for treating T cell cancers. For example, a composition containing one or more bispecific molecules can be administered to a mammal having a T cell cancer to treat the mammal. For example, methods and materials for using one or more bispecific molecules to treat a mammal having a T cell cancer are provided.

公开(公告)号：US20230338297A1

公开(公告)日：2023-10-26

申请号：US18024293

申请日：2021-08-31

Applicant: THE JOHNS HOPKINS UNIVERSITY

Inventor： Jordina RINCON-TORROELLA ,  Bert Vogelstein ,  Kenneth W. Kinzler ,  Shibin Zhou ,  Nickolas Papadopoulos ,  Marco Dal Molin ,  Surojit SUR

IPC: A61K9/50 ,  A61P35/00 ,  A61K45/06 ,  G01N33/50 ,  A61K31/19

CPC classification number: A61K9/5036 ,  A61P35/00 ,  A61K45/06 ,  G01N33/5017 ,  A61K31/19

Abstract: The present disclosure provides compositions comprising at least one cyclodextrin and at least one cytotoxic receptor binding small-molecule. Also disclosed are kits containing said compositions. The compositions of the present disclosure can be administered to a subject suffering from at least one type of cancer.

公开(公告)号：US11718668B2

公开(公告)日：2023-08-08

申请号：US17739274

申请日：2022-05-09

Applicant: The Johns Hopkins University

Inventor： Luis Diaz ,  Bert Vogelstein ,  Kenneth W. Kinzler ,  Nickolas Papadopoulos ,  Dung Le ,  Drew M. Pardoll ,  Suzanne L. Topalian

IPC: G01N31/00 ,  G01N33/53 ,  C07K16/28 ,  C12Q1/6886 ,  C07K16/30 ,  C07K16/40 ,  A61K39/00

CPC classification number: C07K16/2803 ,  C07K16/2818 ,  C07K16/2827 ,  C07K16/30 ,  C07K16/40 ,  C12Q1/6886 ,  C12Y113/11052 ,  A61K2039/505 ,  A61K2039/55 ,  C07K2317/00 ,  C07K2317/24 ,  C07K2317/76 ,  C12Q2600/106 ,  C12Q2600/156

Abstract: Blockade of immune checkpoints such as cytotoxic T-lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1) shows promise in patients with cancer. Inhibitory antibodies directed at these receptors have been shown to break immune tolerance and promote anti-tumor immunity. These agents work particularly well in patients with a certain category of tumor. Such tumors may be particularly susceptible to treatment because of the multitude of neoantigens which they produce.

公开(公告)号：US11628204B2

公开(公告)日：2023-04-18

申请号：US16957970

申请日：2018-12-11

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Nickolas Papadopoulos ,  Shibin Zhou ,  Verena Staedtke ,  Renyuan Bai ,  Gregory J. Riggins

IPC: A61K31/137 ,  A61K31/195 ,  A61K31/198 ,  A61K31/517 ,  A61K38/22 ,  A61P19/02 ,  A61P37/00 ,  A61P37/06

Abstract: This document provides methods and materials for preventing cytokine release syndrome (CRS). For example, methods and materials for using one or more catecholamine inhibitors to prevent a mammal from developing CRS are provided.

公开(公告)号：US20230051847A1

公开(公告)日：2023-02-16

申请号：US17783506

申请日：2020-12-17

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Emily Han-Chung Hsiue ,  Jacqueline Douglass ,  Michael S. Hwang ,  Alexander Pearlman ,  Nickolas Papadopoulos ,  Shibin Zhou ,  Brian Mog ,  Katharine M. Wright ,  Sandra B. Gabelli

IPC: C07K16/30 ,  C07K16/28 ,  A61P35/00

Abstract: This document provides methods and materials for assessing a mammal having or suspected of having cancer and/or for treating a mammal having cancer. For example, molecules including one or more antigen-binding domains (e.g., a single-chain variable fragment (scFv)) that can bind to a modified peptide (e.g., a tumor antigen), as well as method for using such molecules, are provided.

公开(公告)号：US11459611B2

公开(公告)日：2022-10-04

申请号：US16417817

申请日：2019-05-21

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Nickolas Papadopoulos ,  Isaac A. Kinde

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6806

Abstract: The identification of mutations that are present in a small fraction of DNA templates is essential for progress in several areas of biomedical research. Though massively parallel sequencing instruments are in principle well-suited to this task, the error rates in such instruments are generally too high to allow confident identification of rare variants. We here describe an approach that can substantially increase the sensitivity of massively parallel sequencing instruments for this purpose. One example of this approach, called “Safe-SeqS” for (Safe-Sequencing System) includes (i) assignment of a unique identifier (UID) to each template molecule; (ii) amplification of each uniquely tagged template molecule to create UID-families; and (iii) redundant sequencing of the amplification products. PCR fragments with the same UID are truly mutant (“super-mutants”) if ≥95% of them contain the identical mutation. We illustrate the utility of this approach for determining the fidelity of a polymerase, the accuracy of oligonucleotides synthesized in vitro, and the prevalence of mutations in the nuclear and mitochondrial genomes of normal cells.