公开(公告)号：US20240352518A1

公开(公告)日：2024-10-24

申请号：US18682716

申请日：2022-08-12

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Nickolas Papadopoulos ,  Austin Mattox ,  Joshua David Cohen ,  Yuxuan Wang

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6844

CPC classification number: C12Q1/6874 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12Q2600/154

Abstract: Provided herein are methods for identifying a genetic characteristic, a fragment characteristic and an epigenetic characteristic of a double-stranded DNA molecule in a population of double-stranded DNA molecules by assaying both strands of the double-stranded DNA molecule.

公开(公告)号：US20250019755A1

公开(公告)日：2025-01-16

申请号：US18899799

申请日：2024-09-27

Applicant: The Johns Hopkins University

Inventor： Nickolas Papadopoulos ,  Kenneth W. Kinzler ,  Bert Vogelstein ,  Joshua David Cohen

IPC: C12Q1/6855 ,  C12Q1/6876

Abstract: Provided herein are systems, kits, compositions and methods for sequencing library preparation and sequencing workflow (e.g., for the identification of mutations). In certain embodiments, provides herein systems and methods to identically barcode both strands of templates, and PCR-based enrichment of each strand that does not require hybridization capture.

公开(公告)号：US20220073977A1

公开(公告)日：2022-03-10

申请号：US17174838

申请日：2021-02-12

Applicant: The Johns Hopkins University

Inventor： Nickolas Papadopoulos ,  Kenneth W. Kinzler ,  Bert Vogelstein ,  Joshua David Cohen

IPC: C12Q1/6855 ,  C12Q1/6876

Abstract: Provided herein are systems, kits, compositions and methods for sequencing library preparation and sequencing workflow (e.g., for the identification of mutations). In certain embodiments, provides herein systems and methods to identically barcode both strands of templates, and PCR-based enrichment of each strand that does not require hybridization capture.